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Brian William Wilson Grout

Brian William Wilson Grout

Professor emeritus

Uddannelse

BSc i Botany & Cell Biology, Kings College, Univ. of London, 1969. Ph.d. i Plant Biotechnology at University of Nottingham, 1973

Litteraturliste

Scientific & Technical Publications Professor Brian W.W. Grout As of 10.06.2009: 93 peer-reviewed publications 42 books and contributions to edited volumes 29 published conference abstracts and/or proceedings Peer reviewed publications 1. Willison, J.H.M., Grout, B.W.W. and Cocking, E.C. A mechanism for the pinocytosis of latex spheres by isolated tomato fruit protoplasts. Bioenergetics 2, 371-382,1971 2. Grout, B.W.W., Willison, J.H.M. and Cocking, E.C. Interactions at the surface of plant cell protoplasts: an electrophoretic and freeze-etch study. Bioenergetics 4, 585-602,1972 3. Grout, B.W.W. and Coutts, R.H.A. Additives for the enhancement of fusion and endocytosis in higher plant protoplasts: an electrophoretic study. Plant Science Letters 2, 397-403, 1974 4. Coutts, R.H.A. and Grout, B.W.W. Early stages in the cell wall regeneration of tobacco mesophyll protoplasts: an electrophoretic study. Plant Science Letters 4, 103-107, 1975 5. Grout, B.W.W. Cellulose microfibril deposition at the plasmalemma surface of regenerating tobacco mesophyll protoplasts: a deep-etch study. Planta 123, 275-282, 1975 6. Grout, B.W.W. Wax development on the leaf surface of Brassica oleracea regenerated from meristem culture. Plant Science Letters 5, 401-405 1975 7. Grout, B.W.W., Chan, K. W. and Simpkins, I. Aspects of growth and metabolism in a suspension culture of Acer pseudoplatanus L. grown on a glycerol carbon source. J. Exp. Bot. 27, 77-86,1976 8. Grout, B.W.W. and Aston, M.J. Transplanting of cauliflower plants regenerated from meristem culture 1. Water loss and water transfer related to changes in leaf wax and xylem regeneration. Hort. Res. 17, 1-7, 1977 9. Grout, B.W.W. and Aston, M.J. Transplanting of cauliflower plants regenerated from meristem culture 2. Carbon dioxide fixation and the development of photosynthetic ability. Hort.Res.17, 65-71,1977 10. Grout, B.W.W., Westcott, R.J. and Henshaw, G.G. Scanning electron microscope studies of multiple shoot production by meristem tip cultures of Solanum x curtilobum. Ann. Bot. 41, 1113-1116, 1977 11. Grout, B.W.W. and Crisp, P .C. Practical aspects of the propagation of cauliflower by meristem culture. Acta Hort. 78, 289-296, 1977 12. Westcott, R.J., Henshaw, G.G, Grout, B.W.W. and Roca, W. Tissue culture methods and germplasm storage in potato. Acta Hort. 78, 45-49, 1977 13. Mumford, P.M. and Grout, B.W.W. Germination and liquid nitrogen storage of cassava seed. Ann. Bot. 42, 255-257, 1978 14. Grout, B.W.W., Westcott, R.J. and Henshaw, G.G. Survival of shoot meristems of tomato seedlings frozen in liquid nitrogen. Cryobiology 15, 478-483, 1978 15. Willison, J.H.M. and Grout, B.W.W. Further observations on cell wall formation around isolated plant protoplasts. Planta 140, 53-58, 1978 16. Grout, B.W.W. and Aston, M.J. Modified leaf anatomy of cauliflower plantlets regenerated from meristem culture. Ann. Bot. 42, 993-995, 1978 17. Grout, B.W.W. and Henshaw, G.G. Freeze preservation of potato shoot- tip cultures. Ann. Bot. 42, 1227-1229, 1978 18. Weatherhead, M.A., Grout, B.W.W. and Henshaw, G.G. Advantages of the storage of potato pollen in liquid nitrogen. Potato Research 21, 97 -100, 1978 19. Grout, B.W.W. and Mumford, P .M. Desiccation and low temperature tolerance of the recalcitrant seed Citrus limon L. Seed Science and Technology 7, 407-410, 1979 20. Weatherhead, M.A. and Grout, B.W.W. A scanning electron microscope study of haploid plant development in anther cultures of N. tabacum. Ann. Bot. 44, 631-634, 1979 21. Grout, B.W.W. Low temperature storage of imbibed tomato seeds: a model for recalcitrant seed storage. Cryoletters 1, 71-76, 1979 22. Grout, B.W.W. and Henshaw, G.G. Structural observations on the growth of potato shoot-tip cultures after thawing from liquid nitrogen. Ann. Bot. 46, 243-248, 1980 23. Grout, B.W.W. and Williams, A.C. Extra-f1oral nectaries in the genus Dioscorea. Ann. Bot. 46, 255-258 24. Grout, B.W.W. and Crisp, P.C. The origin and nature of shoots derived from caulif1ower roots. J. Hort. Sci. 55, 65-70, 1980 25. Grout, B.W.W., Morris, J.G. and Clarke, A. Changes in the plasmalemma of Chlamydomonas following freezing and thawing. Cryoletters 1, 251-256, 1980 26. Morris, G.J., Clarke, A.C. and Grout, B.W.W. The cryopreservation of Chlamydomonas reinhardtii and studies on the biochemistry of freezing injury. Cryobiology 17, 624, 1980 27. Holmstrom, W. F., Grout, B.W.W. and Morgan, E. Preliminary observations on the low temperature tolerance of the estuarine amphipod Corophium volutator (Pallas). Cryoletters 2, 129-134, 1981 28. Castledine, P., Grout, B.W.W. and Roberts, A. V. Cuticular resistance to Diplocarpon rosae. Trans. Brit. Mycol. Soc. 77, 29-39, 1981 29. Pritchard, H. W., Grout, B.W.W, Reid, D.S. and Short, K. C. An investigation into the effects of growth under water stress on the structure, metabolism and cryopreservation of cultured sycamore cells. Hoppe-Seylers Zeitschrift fur Physiol. Chem. 362, 1190-1191, 1981 30. Grout, B.W.W., Shelton, K., Cou1son, G. and Morris, G.J. Changes in the metabolism and structure of Chlamydomonas reinhardtii (CW15+) following freezing and thawing. Cryoletters 2, 393 -400, 1981 31. Grout, B.W.W. and Morris, G.J. Aspects of the involvement of membranes in cryo-injury. Zeitschrift Probl. Haematol. Transfusion and Transplantation 8, 81-87, 1981 32. Fuller, B.J. and Grout, B.W.W. Cryopreservation of organ-derived cells for transplantation. Zeitschrift Probl. Haematol. Transfusion and Transplantation 8, 871-87, 1981 33. Grout, B.W.W. and Fuller, B.J. Hepatocyte plasma membrane response to hypertonic stress and related cell viability. Cryoletters 3, 51-56.1982 34. Weatherhead, M.A., Grout, B.W.W. and Short, K.C. Increased haploid production in Saintpaulia ionantha by anther culture. Scientia Hort. 17, 137-144. 1982 35. Fuller, B.J. Grout, B.W.W. and Woods. R.J. Biochemical and ultrastructural examination of cryopreserved hepatocytes in rat. Cryobiology 19, 493-502, 1982 36. Fuller, B.J., Morris, G.J., Grout, B.W.W., Bernard, A., Farrant. J., Pritchard., H. W. and McLellan, M. Cold shock -a common cellular injury? Cryobiology 19, 665-666, 1982 37. Grout, B.W.W., Crisp. P.C., and Wardle, K. Frost resistance in cauliflowers. Crop Research 22, 63-65, 1982 38. Castledine, P., Grout, B.W.W. and Roberts, A.V. Potential for long-term storage of Diplocarpon rosae conidia in liquid nitrogen. Trans. Brit. Mycol. Soc. 79, 556-557, 1982 39. Khokhar, J., Humphreys, J.M., Short, K.C. and Grout, B.W.W. Anthocyanins in African Violet. Hortscience 17, 810-812, 1982 40. Grout, B.W.W., Shelton, K. and Pritchard, H. W. Orthodox behaviour of oil palm seed and cryopreservation of the excised embryo for genetic conservation. Ann. Bot. 52, 381-384,1983 41. Morris, G.J., Coulson, G., Meyer, M.A., McLellan. M., Fuller, B.J. Grout, B.W.W., Pritchard, H. W. and Knight, S.C. Cold shock -a widespread cellular reaction. Cryoletters 4, 179-192, 1983 42. James, E., Fuller, B.J., Grout, B.W.W. and Innes, G. Investigation into the causes of lethal damage during attempted cryopreservation of isolated hepatocytes. Cryobiology 20, 718-719, 1983 43. Morris, G.J. and Grout, B.W.W. Membrane vesiculation -a general cellular response to stress. Cryoletters 5, 162-164, 1984 44. Fuller, B.J., Grout, B.W.W. and James, E. Cell membrane surface area changes related to metabolic and osmotic behaviour in isolated rat hepatocytes after freezing and hypertonic exposure. Cryoletters 5, 239-254, 1984 45. Crisp, P.C. and Grout, B.W.W. Storage of broccoli pollen in liquid nitrogen. Euphytica 33, 819-823, 1984 46. Grout, B.W.W. and Millam, S. Photosynthetic development of micropropagated strawberry plantlets following transplanting. Ann. Bot. 55, 129- 131,1985 47. Grout, B.W.W. and Crisp, P.C. Germination as an unreliable indicator of the effectiveness of cryopreservative procedures for imbibed seeds. Ann. Bot. 55, 289-292,1985 48. Pritchard, H.W., Grout, B.W.W. & Short, K.C. Osmotic stress as a pregrowth procedure for cryopreservation 1. Growth and ultrastructure of sycamore and soybean cell suspensions. Ann. Bot. 57, 41-48, 1986 49. Pritchard, H.W., Grout, B.W.W. & Short, K.C. Osmotic stress as a pregrowth procedure for cryopreservation 2. Water relations & metabolic status of sycamore and soybean cell suspensions. Ann. Bot. 57, 371-378, 1986 50. Pritchard, H.W., Grout, B.W.W. & K.C. Short. Osmotic stress as a pregrowth procedure for cryopreservation 3. Cryobiology of sycamore and soybean cell suspensions. Ann. Bot. 57, 379-388, 1986 51. Roberts, S, Grout, B.W.W. and Morris, G.J. Consecutive observations of a frozen cell sample by cryogenic light microscopy and cryogenic scanning electron microscopy. Cryoletters 8, 122-129, 1987 52. Grout, B.W.W and Donkin, M. Photosynthetic activity of cauliflower meristem cultures in vitro and at transplanting into soil. Acta Hort. 212, 323-327, 1987 53. Ryan, K.P., Bateson, J.M., Grout, B.W.W., Purse, D.H. and Wood, J.W. Ice crystal artefacts in frozen specimens as revealed by cryo-scanning electron microscopy. Cryoletters 9, 418-425,1988 54. Grout, B.W.W. Photosynthesis of regenerated plantlets in vitro and the stresses of transplanting. Acta Hort. 230, 129-135, 1988 55. Roberts, S., Morris, G.J., Coulson, G and Grout, B.W.W. A consecutive cryoLM/cryoSEM technique and its use in the study of freezing injury in Chlamydomonas reinhardtii (CWI5+), Cryobiology 25, 534-535, 1988 56. White, G.G., Grout, B.W.W. and Fuller, M.P. Patterns of ice nucleation and lethal injury in floral organs of overwintered cauliflower. Cryobiology 25, 535-536, 1989 57. Fuller, M.P., Grout, B.W.W. and Tapsell, C.R. The patterns of frost- hardening of leaves of winter cauliflower ( Brassica oleracea var. botrytis cv. Clause 30) Ann. Appl. Biol. 115, 161-170, 1989 58. Fuller, M.P., Turton, S. & Grout, B.W.W. Anther culture of winter- heading cauliflower. Acta Hort. 280, 329-332. 1990 59. Grout, B.W.W., Morris, G.J. and McLellan, M.R. Cryopreservation and the maintenance of cell lines. Trends in Biotechnology 8, 293-297, 1990 60. Tapsell, C.R., Grout, B.W.W. & Fuller, M.P. Thermal insulation of the winter cauliflower by curd wrapper leaves. Ann. Appl. Biol. 117. 421-430, 1990 61. Grout, B.W.W. Conservation in vitro. Acta Horticulturae 289, 171-178. 1991 62. Debergh, P., Aitken-Christie, J., Cohen, D., Grout, B.W.W., von Arnold, S., Zimmerman, R. & Ziv, M. Reconsideration of the term 'vitrification' as used in micropropagation. Plant Cell, Tissue and Organ Culture .30, 135-140, 1992 63. Grout, B. W. W. Genetic Preservation of Plant Cells in Vitro. Acta Physiol. Plantarum 18(1): 97-98. 1996. 64. Bishop, C.F.H., Grout, B.W.W., Akrivousi, A. and Makwabara, S.C. Interactions between knife sharpness and storage temperature on browning at the cut surface of hand-trimmed bobby beans ( Phaseolus vulgaris). Acta Hort. (ISHS) 599:129-132. 2003. 65. Zhao, Y., Grout, B.W.W. and Crisp, P.C. Inadvertent selection for unwanted morphological forms during micropropagation adversely affects field performance of European rhubarb ( Rheum rhaponticum). Acta Hort. (ISHS) 616:301-308. 2003 66. Johnson, T.P.C., Grout, B.W.W., Bishop, C.F.H. and Perera, A.. The use of radiant heat to reduce the inoculum level of silver scurf ( Helminthosporium solani) on potato tubers ( Solanum tuberosum) before storage. Acta Hort. (ISHS) 619:207-211. 2003 67. Grout, B.W.W. and Beale, C.V. The positive influence of year-round reflective mulch on apple yield and quality in commercial orchards. Acta Hort. (ISHS) 636: 513-519. 2004 68. Zhao, Y., Grout, B.W.W. and Crisp, P.C. Unexpected susceptibility of novel breeding lines of European rhubarb [ Rheum rhaponticum] to leaf and petiole spot diseases., Acta Hort. (ISHS) 637: 139-144. 2004 69. Taylor, R.D., Grout, B.W.W. and Hill, J. Use of a tensiometer-based control system to reduce irrigation of cut flower Dianthus caryophyllus 'Santorini' whilst maintaining flower yield and quality. Acta Hort. (ISHS) 664: 647-652. 2004 70. Zhao, Y., Green, J., Yu, M. and Grout, B.W.W. In vitro plant regeneration of ornamental cabbage ( Brassica oleracea var. longata). Propagation of Ornamental Plants 5(2): 3-6. 2005 71. Zhao, Y., Grout, B.W.W and Crisp, P.C. Variations in morphology and disease susceptibility of micropropagated rhubarb ( Rheum rhaponticum) PC49, compared to conventional plants. Plant Cell, Tissue and Organ Culture 82: 357-361. 2005 72. Zhao, Y., Grout, B.W.W and Roberts A.V. Abnormal chromosomes and DNA content in micropropagated rhubarb ( Rheum rhaponticumv L .) PC49. Plant Cell, Tissue and Organ Culture 83: 335-338. 2005 73. Zhao, Y., Grout B.W.W. and P.C. Crisp. Unexpected high susceptibility of micropropagated rhubarb ( Rheum rhaponticum L.) to spot disease caused by Ramularia rhei. Communications in Agricultural and Applied Biological Sciences 70(1): 75-79. 2005 74. Johnston, J.W., Harding, K., Bremner, D.H., Souch, G., Green, J., Lynch, P.T., Grout, B.W.W. and Benson, E.E. HPLC Analysis of DNA Methylation in Plants. Plant Physiology & Biochemistry 43(9): 844-853. 2005 75. Zhao, Y., Grout, B.W., Xu, X. Effects of temperature on germination and hyphal growth from conidia of Ramularia rhei and Ascochyta rhei, causing spot diseases of rhubarb ( Rheum rhaponticum) Plant Pathology 55 (5), 664-670. 2006 76. Zhao, Y., Zhou, Y., Grout, B.W.W. Variation in leaf structures of micropropagated rhubarb (Rheum rhaponticum L.) PC49 Plant Cell, Tissue and Organ Culture 85 (1), 115-121. 2006 77. Faszer, K., Draper, D., Green, J.E., Morris G.J. and Grout B.W.W. Cryopreservation of horse semen under laboratory and field conditions using a Stirling Cycle cryocooler. CryoLetters 27(3): 179-184. 2006 78. Zhao, Y., Green, J., Yu, M. and Grout, B.W.W. Histology of adventitious shoot production in micropropagated walking stick cabbage ( Brassica oleracea var.longata). Acta Horticulturae 725, 231-234. 2006 79. Exadaktylou, E., Grout, B., Thomidis, T and Tsipouridis, C. Factors influencing the rooting of Gisela 5 ( Prunus cerasus × Prunus canescens) cherry rootstock hardwood cuttings. Australian Journal of Experimental Agriculture 46 (12), 1633 - 1637. 2006 80. Msogoya, T. J., Maerere, A.P. and Grout, B.W. Field performance of micropropagated East African banana ( Musa AAA East Africa) in the N E zone of Tanzania. Biotechnology 5 (4), 471-474. 2006 81. Exadaktylou, E., Thomidis, T., Grout, B. and Tsipouridis, C. Methods to propagate the cherry rootstock Gisela 5 by using root cuttings and application of micrografting. Advances in Horticultural Science 21(1), 51-54. 2007 82. Morris, G.J., Faszer, K., Green, J., Draper, D., Grout, B.W.W. and Fonseca, F. Rapidly cooled horse spermatozoa: loss of viability is due to osmotic imbalance during thawing, not intracellular ice formation. Theriogenology 68, 804-812. 2007 83. Nyalala, S. and Grout, B.W.W. African spider flower ( Cleome gynandra L.) as a spider mite ( Tetranychus urticae Koch) repellent in cultivated roses ( Rosa hybrida L.). Scientia Horticulturae 114, 194-198. 2007 84. Zhao, Y., Zhou, Y. and Grout, B.W.W.. Crop failure of micropropagated rhubarb ( Rheum rhaponticum l.) PC49 caused by somaclonal variation. Acta Hort. 764:13-20. 2007 85. Zhao,Y., Zhou, Y. and Grout, B.W.W. Alterations in flower and seed morphologies and meiotic chromosome behaviors of micropropagated rhubarb (Rheum rhaponticum L.) 'PC49'.Scientia Horticulturae 116, 162-168. 2008 86. Taylor, R.D., Grout, B.W.W. and Hill, J. Does Irrigation Regime Affect the Post Harvest Quality and Vase Llife of Cut Flower Carnations (cv. Santorini). Acta Hort. 792: 663-668. 2008 87. Msogoya, T., Grout, B. and Roberts, A. Karyotypic and 2c nuclear DNA size instability in in vitro induced off-types of East African highland bananas (Musa AAA East Africa). Biotechnology 7(3): 578-581. 2008 88. Msogoya, T. and Grout, B. Altered response to biotic and abiotic stress in tissue culture-induced off-type plants of East African highland bananas. J. Applied Sciences 8(15):2703-2710. 2008 89. Exadaktylou E, Thomidis T, Grout B. Methods to improve the rooting of Gisela 5 hardwood cuttings. HorTechnology 19(2): 254-259. 2009 90. Grout, B.W.W. and Morris, G.J. contaminated liquid nitrogen vapour as a risk factor in pathogen transfer. Theriogenology 71: 1079-1082. 2009 In Press 91. Zhao, Y., Grout, B.W.W. and Crisp, P.C. Screening Morphological Variation in Micropropagated Plants of Rhubarb ( Rheum rhaponticum) Derived from Uniform, Acclimated Plantlets. Acta Horticulturae 92. Taylor, R.D., Grout, B.W.W. and Hill, J. Assessment of petal colour change in cut-flower carnation (cv. Santorini) during vase life. Acta Hort. 93. Taylor, R.D., Grout, B.W.W., Hill, J. and Brough, W. The Use of Cold Storage as a Scheduling Aid in Bedding Plant Production. Acta Hort Under review Green, J., Grout, B.W.W. and Benson , E. Low-technology cryopreservation of overwintering Ribes nigrum L. buds. - Cryoletters. Books & contributions to edited volumes 1. Grout, B.W.W. and Short K.C. Fundamentals of PIant Tissue Culture, pp 48 , Grower Books, London, 1979 2. Jackson, M.B., Grout, B.W.W. and Mackenzie, I.A. [Editors] Growth Regulators in Plant Senescence -Monograph 8 , pp 132, British Plant Growth Regulator Group, Wantage UK, 1982 3. Grout, B.W.W. & Morris G.J. [Editors] The Effects of Low Temperatures on Biological Systems, pp 487, Edward Arnold, London, 1987 4. Fuller, B.J. & Grout, B.W.W. (Editors] Clinical Applications of Cryobiology, pp 246, CRC Press, Boca Raton USA, 1991 5. Grout, B.W.W. [Editor] Genetic Preservation of Plant Cells in vitro, pp 169, Springer Verlag, Berlin, 1995 6. Henshaw, G.G. and Grout, B.W.W. The long term storage of plant tissues by meristem culture and other in vitro techniques. In "Conservation of plant genetic resources" edited by J.G. Hughes, pp 36-41, British Association for the Advancement of Science, 1978 7. Westcott, R.J., Grout, B.W.W. and Henshaw, G.G. Rapid clonal propagation of Solanum curtilobum var. Malku. by aseptic shoot meristem culture. in "The Biology and Taxonomy of the Solanaceae" edited by G.J. Hawkes, R.N. Lester and A.D. Skelding, pp 377-382, Academic Press, 1979 8. Grout, B.W.W., Willison, J.H.M. and Cocking, E.C. Interactions at the surface of plant protoplasts. In "Membrane Structure and Mechanisms of Biological Energy Transduction", edited by I. Avery, pp 595-612, Plenum Press, 1974 9. Grout, B.W.W. and Weatherhead, M.A. A strategy for the production of disease-resistant mutants. In "Tissue Culture Methods for Plant Pathologists' edited by D.S. Ingram and J.P. Hegelson, pp249-254, Blackwell, 1980 10. Grout, B.W.W. and Short, K.C. Cryobiology and the storage of mammalian embryos. In "Integrated Medicine" edited by B. Stacey, pp 605-610, Van Nostrand Reinhold, 1981 11. Roberts, A. V., Castledine, P. and Grout, B.W.W. Resistance and susceptibility to Blackspot. in "The Rose Annual 1981 ", edited by J. Harkness, pp I17-121, Royal National Rose Society, 1981 12. Morris, G.J., Coulson, G.E., Clarke, K.J., Grout, B.W.W. and Clarke A.C. Freezing injury in Chlamydomonas; a synoptic approach. In "Effects of Low Temperatures on Biological Membranes" edited by G.J. Morris and A.C. Clarke, pp 285-306, Academic Press, 1981 13. Pritchard. H.W., Grout, B.W.W., Short, K.C. and Reid. D.S. The effects of growth under water stress on the structure. metabolism and cryopreservation of cultured sycamore cells. In "Biophysics of Water" edited by F. Franks and S. Mathias, 319-322, Wiley, 1982 14. Grout, B.W.W. Experience with disposable cuvettes. Laboratory Practice 33, 24-27, 1984 15. Grout, B.W.W. Embryo culture and cryopreservation for the conservation of genetic resources of species with recalcitrant seed. In "Plant Tissue Culture and its Agricultural Applications" edited by L.A. Withers & P.G. Alderson, pp 303-309, Butterworths, 1986 16. Grout, B.W.W. Freezing and higher plants. Endeavour 10, 203-207, 1987 17. Grout, B.W.W. Vortschade aan planten. Natuur & Techniek 55, 248- 255,1987 18. Grout, B.W.W. Higher plants at freezing temperatures. In "The Effect of Low Temperatures on Biological Systems" edited by B.W.W. Grout & G.J. Morris, pp 293-314, Edward Arnold, London, 1987 19. Grout, B.W.W. & Morris, G.J. Freezing and cellular organisation. In "The Effects of Low Temperatures on Biological Systems" edited by B.W.W.Grout and G.J. Morris, pp 147-174, Edward Arnold. London, 1987 20. Bajaj, Y.P.S., Davey, M. and Grout, B.W.W. Pollen tetrad protoplasts as a model system for the ontogeny of pollen. In "Gamete Competition in Plants and Animals" edited by D.L. Mulcahy, pp 7-18, North Holland, 1975 21. Grout, B.W.W. Cauliflower ( Brassica oleracea var. botrytis) In "Biotechnology in Agriculture and Forestry 6, Crops II" edited by Y.P.S. Bajaj, pp 209-225, Springer Verlag, Heidelberg, 1988 22. Grout, B.W.W. Le piante e il gelo. Scienza & Technica 87/88, 181-187, 1988 23. Grout, B.W.W. Vortschade aan planten. Landbouwleven 38,10-11, 1988 24. Grout, B.W.W. African Violet. In "Handbook of Plant Cell Culture 5.-- Ornamental Species" Edited by P. Ammirato, D. Evans, W.I. Sharp and Y.P.S. Bajaj, ppI81-20S, McGraw Hill, USA, 1989 25. Grout, B.W.W. Meristem-tip culture. In" Plant Cell and Tissue Culture. Methods in Molecular Biology 6". edited by J. W .Pollard and J.M. Walker, pp 81-92, Humana Press USA, 1990 26. Grout, B.W.W. Genetic preservation in vitro. In "Progress in Plant Cellular and Molecular Biology". Edited by H.J.J. Nijkamp, L.H.W. van der Plas and J. van Aartrijk, pp13-22, Kluwer Academic Publishers, Netherlands, 1990 27. Grout, B.W.W. In vitro conservation of germplasm. In" Plant Tissue Culture: Applications and Limitations". Edited by S.S. Bhojwani, pp 394-411, Elsevier, Netherlands, 1990 28. Grout, B.W.W. & Cross. J. Refurbishing the forest. Laboratory Practice 40, 22-23, 1991 29. Grout, B.W.W. "The effects of ice formation during the cryopreservation of clinical systems. In "Clinical Applications of Cryobiology". Edited by B.J. Fuller and B.W.W. Grout, pp 81-94, CRC Press, Boca Raton USA, 1991 30. Grout, B.W.W. & D'Orey, B. Micropropagacao o processo exige novas solucoes. Lavora Portuguesa 91, 36-41, 1991 31. Grout, B.W.W. [Editor] Genetic Preservation of Plant Cells in vitro, pp 169, Springer Verlag, Berlin, 1995 32. Grout, B.W.W. Introduction to the in vitro preservation of plant cells, tissues and organs. In "Genetic Preservation of Plant Cells in vitro" edited by B.W.W. Grout, pp 1- 20, Springer Verlag, Berlin, 1995 33. Grout, B.W.W. Minimal growth storage. In "Genetic Preservation of Plant Cells in vitro" edited by B.W.W. Grout, pp21-28, Springer Verlag, Berlin, 1995 34. Grout, B.W.W. Cryopreservation of protoplast, suspension and callus cultures. In "Genetic Preservation of Plant Cells in vitro" edited by B.W.W. Grout, pp 29-46, Springer Verlag, Berlin, 1995 35. Grout, B.W.W. Cryopreservation of excised meristems, shoot tips and somatic embryos. In "Genetic Preservation of Plant Cells in vitro" edited by B.WW. Grout, pp 47-62, Springer Verlag, Berlin, 1995 36. Grout, B.W.W. & Roberts, A.V. Storage of free pollen, pollen embryos and the zygotic embryos of seed by cryopreservation and freeze-drying. In "Genetic Preservation of Plant Cells in vitro" edited by B.W.W Grout, pp 63-74, Springer Verlag, Berlin, 1995 37. Grout, B.W.W. & Crisp, P.C. Cryopreservation of germplasm of tomato. In "Biotechnology in Agriculture & Forestry (32). Cryopreservation of plant germplasm" edited by Y.P.S. Bajaj, pp 371-380, Springer Verlag, Berlin, 1995 38. Grout, B.W.W. Low temperature storage of plant tissue cultures. In "Automation & Environmental Control in Plant Tissue Culture" edited by J Aitken-Christie, T Kozai & M. Lila Smith, pp 517-538, Kluwer Academic Publishers. Holland, 1995 39. Grout, B.W.W. Cryopreservation of plant protoplasts. In " Methods in Molecular Biology 38 - Cryopreservation and Freeze-drying Protocols" edited by J.G. Day & M.R. McLellan, pp 91-102, Humana Press, New Jersey, 1995 40. Grout, B.W.W. Meristem-tip culture for propagation and virus elimination. In "Methods in Molecular Biology 111- Plant Cell Culture Protocols" edited by R. Hall, pp 115-126, Humana Press, New Jersey, 1999 41. Grout, B.W.W. Agriculture entries. In "Chambers Dictionary of Science & Technology (2 nd edn.)" edited by J. Lackie. Chambers Harrap, Edinburgh, 2006 42. Grout, B.W.W. Cryopreservation of plant cell suspensions. In "Methods in Molecular Biology 368 - Cryopreservation and freeze-drying protocols" edited by J.G. Day & G.N.. Stacey, pp 153-161, Humana Press, New Jersey, 2007 Published conference proceedings 1. Grout, B.W.W., Morris, G.J. Clarke, A.C. and Coulson, G. Effects of freezing on properties of the plasma membrane of Chlamydomonas. In "Proceedings of an International Meeting of the Society for Low Temperature Biology" edited by R. Dvorak and F. Bohm, pp 102-105, Czechoslovak Academy of Sciences, Prague, 1981 2. Grout, B.W.W. Cold-hardening and cryoprotection: recovery of stored material. In "Crop Genetic Resources - the Conservation of Difficult Material" edited by L.A. Withers and J.T. Williams. pp 105-108, IBPGR. Rome, 1982 3. Grout, B.W.W. and Crisp. P.C. The selection and exploitation of cauliflowers with increased low temperature tolerance. Cryoletters 3, 327[abstr.] 1982 4. Attenburrow.V., Grout, B.W.W. James. E. and Fuller, B.J. Osmotic stress and cellular function in cryopreserved hepatocytes. Cryoletters 3. 325[abstr.], 1982 5. Pritchard, H. W. and Grout, B.W.W. Effects of growth in hyperosmotic medium on the synthesis, metabolism and cryopreservation of cultured plant cells. Cryoletters 3, 325[abstr.]. 1982 6. Grout, B.W.W., Whitehouse, D.G.W. and Edwards, B.J. Cellular injury resulting from hypertonic stresses imposed upon the mutant unicellular alga Chlamvdomonas reinhardtii [CWI5+]. Cryobiology 20, 719, 1983 7. Short, K.C., Wardle, K., Grout, B.W.W. and Simpkins, I. In vitro physiology and acclimatization of aseptically cultured plantlets. In "Plant Tissue and Cell Culture -Applications to Crop Improvement" edited by F. Novak, L. Havel and J. Dolezel, pp 475-486, Czechoslovak Academy of Sciences, Prague, 1984 8. Warburton, J., Grout, B.W.W. and Short, K.C. In vitro selection for cold tolerant lines of Saintpaulia ionantha (Wendl.). In "Plant Tissue and Cell Culture -Applications to Crop Improvement" edited by F. Novak, L. Havel and J. Dolezel, pp 355-357, Czechoslovak Academy of Sciences, Prague, 1984 9. Grout, B.W.W and Price, F. The establishment of photosynthetic independence in strawberry cultures prior to transplanting. In "Plant Micropropagation in Horticultural Industries" edited by G. Ducote, M. Jacob &.A. Simon, pp55-69, Belgian PTC Group, Aarlon, 1987 10. Bateson, J.M., Grout, B.W.W. and Lane S. The influence of container size on the multiplication rate of regenerating plant cell cultures. In "Plant Micropropagation in Horticultural Industries" edited by G. Ducate, M. Jacob and A. Simon, pp275-277, Belgian PTC Group, Aarlon, 1987 11. Fuller, M.P., Grout B.W.W., White, G.G. and Tapsell, C.R. Frost tolerance and avoidance in winter cauliflower. Cryoletters 9, 431 [Abstr}, 1988 12. Roberts, S. and Grout, B.W.W. An ultrastructural investigation into freezing-induced dehydration of Chlamydomonas reinhardtii (CW15+), Cryobiology 25, 528 ( Abstr) , 1988 13. Bateson, J.M., Grout, B.W.W. and Lane, S.D. High lead resistance in carrot plants following selection of somaclonal variants in vitro. In "Plants and Pollutants in Developed and. Developing Countries" edited by M.A. Ozturk, pp 569-584, Ege University Press, Izmir, Turkey, 1989 14. Morris, G.J. & Grout, B.W.W. Technological synthesis. In "Life at Low Temperatures" Phil. Trans. Roy. Soc. Land. B. 326, 687-692, 1990 15. Grout, B.W.W., Morris, G.J. and McLellan, M.R. Freezing of fruit and vegetables. In "Food Freezing -Today and Tomorrow". edited by W. Bald. pp 113-122, Springer Verlag. Berlin, 1991 16. McLellan, M.R., Morris, G.J., Grout, B.W.W. & Hughes, K.W. Light microscopy of foodstuffs during freezing and thawing. In "Food Freezing -Today and Tomorrow". Edited by W. Bald, pp 171-186. Springer Verlag, Berlin. 1991 1 17. Grout, B.W.W. Cryopreservation of plant cells and organs. In "Conservation of Plant Genetic Resources through in vitro- Methods". Edited by A.H. Zakri, M.N. Normaah, A.G. Abdul Karim and M.T. Senawi, pp 43-56, FRIM/MNCPGR, Kuala Lumpur Malaysia, 1991 18. Grout, B.W.W., Morris, G.J., and McLellan, M.R. Cryopreservation of gametes and embryos of aquatic organisms. In" Biotechnology and the Conservation of Genetic Diversity Symp. Zool. Soc. Lond. 64". Edited by H.D.M. Moore, W.V. Holt and G.M. Mace, pp 63-71, Oxford Univ. Press, 1992 19. Zhao, Y.,, B.W.W. Grout and P.C. Crisp. Identification and characterization of pathogenic fungi causing spot disease on rhubarb ( Rheum rhaponticum L.) in the UK. Communications in Agricultural and Applied Biological Sciences 68(4b) 695-704. 2003. 20. Dangol, D. R.; Grout, B and Perera, A. Ethnobotany and the Traditional Healers of the Tharu Community of the Chitwan Terai, Nepal. 9th International Congress of the International Society of Ethnobiology 2004 http://www.kent.ac.uk/anthropology/ice2004/posters/wedabs2.html 21. Zhao, Y., Grout, B.W.W. and Crisp, P. Variations in leaf, flower and seed morphology and chromosome number in micropropagated plants of rhubarb ( Rheum rhaponticum L.]. Bulletin of Agricultural Sciences and Veterinary Medicine [USAMV-CN] 59: 199-204. 2003 22. Zhao, Y., Grout B.W.W. and P.C. Crisp. Unexpected high susceptibility of micropropagated rhubarb ( Rheum rhaponticum L.) to spot disease caused by Ramularia rhei. Communications in Agricultural and Applied Biological Sciences 70(1): 75-79. 2005 23. Green, J.E., Faszer, K., Draper, D., Morris, G.J. and Grout, B.W. Field preservation of large volumes of equine semen under field conditions: Potential for genetic conservation. Cryobiology 53(3), 378 (Abst.). 2006 24. Draper, D., Green, J.E., Grout, B.W. and Morris, G.J. Alternative strategies for the improvement of cryopreservation of equine spermatozoa. Cryobiology 53(3), 392 (Abst.). 2006 25. Dangol, D.R., Teeling, C. and Grout, B.W.W. Ethnoveterinary medicine and the traditional healers of the Tharu community in the Chitwan Terai of Nepal. Harvesting Knowledge, Pharming Opportunities - Proceedings of an Ethnoveterinary Medicine Conference. British Society of Animal Science. 16-17. 2006 26. Morris J and Grout BW Contaminated liquid nitrogen storage vessels as pathogen vectors Cell Preservation Technology 5(1), 63 (Abst) 2007 27. Grout, B.W.W. and Morris, G.J. Contaminated liquid nitrogen storage vessels as potential vectors for pathogens. CryoLetters 29(1), 74-75 (Abst.).2008 28. Toldam-Andersen, T.B., Krogholm, K.S. and Grout, B.W.W.. Cryopreservation of dormant buds in a genebank collection of Pyrus communis. CryoLetters 29(1), 81-82 (Abst.).2008 29. Grout, B.W.W., Green, J., Benson, E.E., Harding, K. and Johnston, J.W. Cryopreservation of encapsulated ribes meristems and variation between accessions within a cultivar. In "Agrifood Research Working Papers 153 - Cryopreservation of Crop species in Europe" edited by J. Laamamen, M. Uosukainen, H. Haggman, A. Nukari and S. Rantala, p.50, MTT Agrifood Research, Finland, 2008. [ www.mtt.fi/mtts/pdf/mtts153.pdf]

Ansættelser

Professor i Havebrugsvidenskab, Københavns Universitet, fra januar 2007 For tidligere ansættelser: Se venligst CV

Forskning og Undervisning

i havebrugsvidenskab med en stor interesse i bevarelse af den genetiske resources. Specielt interesseret i kryopræservering Studieleder for Kandidatuddannelsen i Havebrugsvidenskab

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