Forskning ved Københavns Universitet - Københavns Universitet

Forside
Hanne Mørck Nielsen

Hanne Mørck Nielsen

Professor

Primære forskningsområder

Drug formulation and delivery of therapeutic peptides/proteins and oligonucleotides especially focussing on studying the interaction with biological membranes related to delivery of the drug. The influence of the expected (inactive) excipients used in advanced drug delivery systems as well as the drug formulation processing parameters on the toxicity and drug targeting to and interaction with, uptake and transport across the biological membranes and intracellular processing are the primary focus and related to the (physical and chemical) stability of the therapeutic drug. A mechanistic approach to understanding the challenges of particulate formulation development for delivery of biopharmaceuticals is the key point of the research. Present projects comprise research in particulate systems as well as the use of (membrane interacting) peptides (such as antimicrobial peptides and cell-penetrating peptides), polymers and lipids for cellular delivery enhancement of biopharmaceuticals investigating the (potential targeting) effect and mechanisms leading to a desired response. For mechanistic assessment purposes, suitable cell culture models and in vivo models are applied.

 

Undervisnings- og vejledningsområder

Main teaching activities:

Drug Delviery course (co-organizer), PhD course

Formulation and Delivery course (MIND course)

Advanced Drug Formulation (master course)

Pharmaceutical formulation of peptides and proteins (master course)

Bachelor, master thesis, and individual unit projects

 

Supervision
Master theses 2013-2014

 

Aktuel forskning

 Projects

COMPACT (http://www.imi.europa.eu/content/compact)

DanCARD (http://www.dancardproject.dk/)

IPN (http://hoejteknologifonden.dk/projektgalleri/projektgalleri/millioner_af_infektioner_paa_sygehuse_kan_forhindres/)

 

 

PhD students

Non-injectable delivery of modified biopharmaceuticals (PhD student Mie Kristensen, 2011-)

Influence of mucosal membrane barrier properties on transport kinetics of oral peptide and protein delivery (Marie Bøgh, 2010 -)

Improved intracellular delivery of antimicrobial peptides by nanomedicine (PhD student Jorrit Water, 2011-)

Impregnation of antimicrobial peptides into a novel composite material - optimizing the interplay between formulation, peptide and material (PhD student Søren Langer Steffensen, 2011-)

A mechanistic approach of membrane interactions of antimicrobial peptides (Jesper Søborg Bahnsen, 2011-)

 

Udvalgte publikationer

  1. Udgivet

    Trehalose preserves DDA/TDB liposomes and their adjuvant effect during freeze-drying

    Christensen, D., Foged, C., Rosenkrands, I., Nielsen, H. M., Andersen, P. & Agger, E. M. 2007 I : Biochimica et Biophysica Acta. 1768, 9, s. 2120-2129

    Publikation: Forskning - peer reviewTidsskriftartikel

  2. Udgivet

    Cell-penetrating peptides for drug delivery across membrane barriers

    Foged, C. & Nielsen, H. M. 2008 I : Expert Opinion on Drug Delivery. 5, 1, s. 105-17 12 s.

    Publikation: ForskningReview

  3. Udgivet

    Phospholipase A2 sensitive liposomes for delivery of small interfering RNA (siRNA)

    Foged, C., Nielsen, H. M. & Frøkjær, S. 2007 I : Journal of Liposome Research. 17, 3-4

    Publikation: Forskning - peer reviewTidsskriftartikel

  4. Udgivet

    Liposomes for phospholipase A2 triggered siRNA release: preparation and in vitro test

    Foged, C., Nielsen, H. M. & Frøkjær, S. 2007 I : Internation Journal of Pharmaceutics. 331, 2, s. 160-166

    Publikation: Forskning - peer reviewTidsskriftartikel

  5. Udgivet

    Farvel til sprøjten: udvikling af vaccineplaster med nanopartikler

    Madsen, H. B., Ifversen, P., Madsen, F., Manniche, S. & Nielsen, H. M. 2007 I : Lægemiddelforskning. s. 12-13

    Publikation: FormidlingTidsskriftartikel

  6. Udgivet

    Polymeric nanocarriers for siRNA delivery: challenges and future prospects

    Cun, D., Jensen, L. B., Nielsen, H. M., Moghimi, S. M. & Foged, C. 2008 I : Journal of Biomedical Nanotechnology. 4, 3, s. 258-275

    Publikation: ForskningReview

  7. Udgivet

    Spray drying of siRNA-containing PLGA nanoparticles intended for inhalation

    Jensen, D. M. K., Cun, D., Maltesen, M. J., Frøkjær, S., Nielsen, H. M. & Foged, C. 2010 I : Journal of Controlled Release. 142, s. 138-145

    Publikation: Forskning - peer reviewTidsskriftartikel

  8. Udgivet

    Design of an inhalable dry powder formulation of DOTAP-modified PLGA nanoparticles loaded with siRNA

    Jensen, D. M. K., Jensen, L. B., Koocheki, S., Cun, D., Nielsen, H. M., Foged, C. & Bengtson, L. 2012 I : Journal of Controlled Release. 157, 1, s. 141-148

    Publikation: Forskning - peer reviewTidsskriftartikel

  9. Udgivet

    Preparation and characterization of poly (DL-lactide-co-glycolide) nanoparticles for siRNA delivery

    Cun, D., Foged, C., Yang, M., Frøkjær, S. & Nielsen, H. M. 2010 I : Internation Journal of Pharmaceutics. 390, 1, s. 70-75

    Publikation: Forskning - peer reviewTidsskriftartikel

  10. Udgivet

    High loading efficiency and sustained release of siRNA encapsulated in PLGA nanoparticles: quality by design optimization and characterization

    Cun, D., Jensen, D. M. K., Maltesen, M. J., Bunker, M., Whitside, P., Scurr, D., Foged, C. & Nielsen, H. M. 2011 I : European Journal of Pharmaceutics and Biopharmaceutics. 77, 1, s. 26-35

    Publikation: Forskning - peer reviewTidsskriftartikel

  11. Udgivet

    a, a´- trehalose 6,6´- dibehenate in non-phospholipid-based liposomes enables direct interaction with trehalose, offering stability during freeze-drying

    Christensen, D., Kirby, D., Foged, C., Agger, E. M., Andersen, P., Perrie, Y. & Nielsen, H. M. 2008 I : Biochimica et Biophysica Acta - Biomembranes. 1778, 5, s. 1365-1373

    Publikation: Forskning - peer reviewTidsskriftartikel

  12. Udgivet

    Dendritiske nanostrukturer som transportører af genestisk medicin

    Jensen, L. B., Nielsen, H. M. & Foged, C. 2008 I : Lægemiddelforskning. s. 13-15

    Publikation: FormidlingTidsskriftartikel

  13. Udgivet

    Cellular uptake and membrane-destabilising properties of a-peptide/ß-peptoid chimeras: lessons for the design of new cell-penetrating peptides

    Foged, C., Franzyk, H., Bahrami, S., Frøkjær, S., Jaroszewski, J. W., Nielsen, H. M. & Olsen, C. A. 2008 I : Biochimica et Biophysica Acta. 1778, 11, s. 2487-2495

    Publikation: Forskning - peer reviewTidsskriftartikel

  14. Udgivet

    Status and future prospects of lipid-based particulate delivery systems as vaccine adjuvants and their combination with immunostimulators

    Nordly, P. J., Madsen, H. B., Nielsen, H. M. & Foged, C. 2009 I : Expert Opinion on Drug Delivery. 6, 7, s. 1-16

    Publikation: ForskningReview

  15. Udgivet

    CAF01 liposomes as a mucosal vaccine adjuvant: In vitro and in vivo investigations

    Christensen, D., Foged, C., Rosenkrands, I., Lundberg, C. V., Andersen, P., Agger, E. M. & Nielsen, H. M. 2010 I : Internation Journal of Pharmaceutics. 390, s. 19-24

    Publikation: Forskning - peer reviewTidsskriftartikel

  16. Udgivet

    NIR transmission spectroscopy for rapid determination of lipid and lyoprotector content in liposomal vaccine adjuvant system CAF01

    Christensen, D., Allesø, M., Rosenkrands, I., Rantanen, J. T., Foged, C., Agger, E. M., Andersen, P. & Nielsen, H. M. 2008 I : European Journal of Pharmaceutics and Biopharmaceutics. 70, 3, s. 914-920

    Publikation: Forskning - peer reviewTidsskriftartikel

  17. Udgivet

    Cell-penetrating peptides for cytosolic delivery of biomacromolecules

    Foged, C., Jing, X. & Nielsen, H. M. 2010 Organelle-specific pharmaceutical nanotechnology. Weissig, V. & D'Souza, G. G. M. (red.). Hoboken: John Wiley & Sons Ltd, s. 403-432

    Publikation: ForskningBidrag til bog/antologi

  18. Udgivet

    Modulation of the adjuvant activity of DDA/TDB liposomes with immunostimulators

    Nordly, P. J., Foged, C., Christensen, D., Lindenstrøm, T., Nielsen, H. M. & Agger, E. 2010

    Publikation: ForskningPoster

  19. Udgivet

    Incorporation of a synthetic mycobacterial monomycoloyl glycerol analogue stabilizes dimethyldioctadecylammonium liposomes and potentiates their adjuvant effect in vivo

    Nordly, P. J., Korsholm, K. S., Pedersen, E. A., Khilji, T. S., Franzyk, H., Jørgensen, L., Nielsen, H. M., Agger, E. M. & Foged, C. 2011 I : European Journal of Pharmaceutics and Biopharmaceutics. 77, 1, s. 89-98

    Publikation: Forskning - peer reviewTidsskriftartikel

  20. Udgivet

    Stabilization of liposomes during drying

    Ingvarsson, P. Þ., Yang, M., Nielsen, H. M., Rantanen, J. T. & Foged, C. 2011 I : Expert Opinion on Drug Delivery. 8, 3, s. 375-388

    Publikation: ForskningReview

  21. Udgivet

    A synthetic mycobacterial monomycoloyl glycerol analogue stabilizes DDA liposomes

    Nordly, P. J., Pedersen, E. A., Khiljii, T. S., Agger, E. M., Jørgensen, L., Nielsen, H. M., Korsholm, K. S., Franzyk, H. & Foged, C. 2010 I : CRS Newsletter. 27, 2, s. 12 14 s.

    Publikation: ForskningTidsskriftartikel

  22. Udgivet

    Incorporation of the TLR4 agonist monophosphoryl lipid A into the bilayer of DDA/DTDB liposomes : physico-chemical characterization and induction of CD8+ T-Cell responses in vivo

    Nordly, P. J., Agger, E. M., Andersen, P., Nielsen, H. M. & Foged, C. 2011 I : Pharmaceutical Research. 28, 3, s. 553-562

    Publikation: Forskning - peer reviewTidsskriftartikel

  23. Udgivet

    Molecular characterization of the interaction between siRNA and PAMAM G7 dendrimers by SAXS, ITC, and molecular dynamics simultations

    Jensen, L. B., Mortensen, K., Pavan, G. M., Kasimova, M. R., Jensen, D. K., Gadzhyeva, V., Nielsen, H. M. & Foged, C. 2010 I : Biomacromolecules. 11, 12, s. 3571-3577

    Publikation: Forskning - peer reviewTidsskriftartikel

  24. Udgivet

    Elucidating the molecular mechanism of PAMAM-siRNA dendriplex self-assembly: Effect of dendrimer charge density

    Jensen, L. B., Pavan, G. M., Kasimova, M. R., Rutherford, S., Danani, A., Nielsen, H. M. & Foged, C. 2011 I : International Journal of Pharmaceutics. 416, s. 410-418

    Publikation: Forskning - peer reviewTidsskriftartikel

  25. Udgivet

    Immunity by formulation design: induction of high CD8+ T-cell responses by poly (I:C) incorporated into the CAF01 adjuvant via a double emulsion method

    Nordly, P. J., Rose, F., Christensen, D., Nielsen, H. M., Andersen, P., Agger, E. M. & Foged, C. 2011 I : Journal of Controlled Release. 150, 3, s. 307-317

    Publikation: Forskning - peer reviewTidsskriftartikel

  26. Udgivet

    Comparison of polymeric siRNA nanocarriers in murine LPS-activated macrophage cell line: Gene silencing, toxicity and off-target gene expression

    Jensen, L. B., Griger, J., Naeye, B., Varkouhi, A. K., Raemdonck, K., Schiffelers, R., Lammers, T., Storm, G., Smedt, S. C. D., Sproat, B. S., Nielsen, H. M. & Foged, C. 2012 I : Pharmaceutical Research. 29, s. 669-682

    Publikation: Forskning - peer reviewTidsskriftartikel

  27. Udgivet

    MRI-assessed therapeutic effects of locally administered PLGA nanoparticles loaded with anti-inflammatory siRNA in a murine arthritis model

    Te Boekhorst, B. C. M., Jensen, L. B., Colombo, S., Varkouhi, A. K., Schiffelers, R. M., Lammers, T., Storm, G., Nielsen, H. M., Strijkers, G. J., Foged, C. & Nicolay, K. 2012 I : Journal of controlled release : official journal of the Controlled Release Society. 161, 3, s. 772-80 9 s.

    Publikation: Forskning - peer reviewTidsskriftartikel

  28. Udgivet

    Microcalorimetric and spectroscopic studies on the mechanism of interaction between novel peptidomimetics and lipid bilayers

    Nielsen, H. M., Jing, X., Simonsen, A., Kasimova, M. R., Malmsten, M., Franzyk, H. & Foged, C. 2010 I : Journal of Peptide Science. 16, s1, s. 151 1 s.

    Publikation: ForskningKonferenceabstrakt i tidsskrift

  29. Udgivet

    Microcalorimetric and spectroscopic studies on the mechanism of interaction between novel peptoids and lipid bilayers - effect of length, charge and N-terminal end group

    Jing, X., Simonsen, A. H., Kasimova, M. R., Franzyk, H., Foged, C. & Nielsen, H. M. 2010 I : Drug Discovery Today. 15, 23/24, s. 1109

    Publikation: ForskningKonferenceabstrakt i tidsskrift

  30. Udgivet

    Innovativt nanopartikeldesign bag skræddersyede vacciner

    Nordly, P. J., Agger, E. M., Nielsen, H. M. & Foged, C. 2009 I : Lægemiddelforskning. s. 19-21

    Publikation: ForskningTidsskriftartikel

  31. Udgivet

    Metabolic cleavage and translocation efficiency of selected cell penetrating peptides: A comparative study with epithelial cell cultures

    Foerg, C., Weller, K. M., Rechsteiner, H., Nielsen, H. M., Fernandez-Carneado, J., Brunisholz, R., Giralt, E. & Merkle, H. P. 2008 I : AAPS Journal. 10, 2, s. 349-359

    Publikation: Forskning - peer reviewTidsskriftartikel

  32. Udgivet

    In Vitro cutaneous application of ISCOMs on human skin enhances delivery of hydrophobic model compounds through the stratum corneum

    Madsen, H. B., Ifversen, P., Madsen, F., Larsen, B. B., Hausser, I. & Nielsen, H. M. 2009 I : AAPS Journal. 11, 4, s. 728-739

    Publikation: Forskning - peer reviewTidsskriftartikel

  33. Udgivet

    Biotechnology-based pharmaceuticals

    Jørgensen, L., Nielsen, H. M. & Frøkjær, S. 2009 Modern pharmaceutics: applications and advances. Florence, A. T. & Siepmann, J. (red.). 5 udg. New York: Informa Healthcare, Bind 2, s. 259-293

    Publikation: ForskningBidrag til bog/antologi

  34. Udgivet

    Influence of skin barrier on in-vitro penetration properties of solid lipid nanoparticles

    Jensen, L. B., Nielsen, H. M. & Petersson, K. 2010 I : Journal of Pharmacy and Pharmacology. s. 793-794

    Publikation: ForskningKonferenceabstrakt i tidsskrift

  35. Udgivet

    Antimicrobial, hemolytic, and cytotoxic activities of ß-peptoid-peptide hybrid oligomers: Improved properties compared to natural AMPs

    Olsen, C. A., Ziegler, H. L., Nielsen, H. M., Frimodt-Møller, N., Jaroszewski, J. W. & Franzyk, H. 2010 I : ChemBioChem. 11, 10, s. 1356-1360

    Publikation: Forskning - peer reviewTidsskriftartikel

  36. Udgivet

    Delivery technologies for biopharmaceuticals: Peptides, proteins, nucleic acids and vaccines

    Jørgensen, L. & Nielsen, H. M. 2009 John Wiley & Sons Ltd. 413 s.

    Publikation: Forskning - peer reviewBog

  37. Udgivet

    Corticosteroid solubility and lilpid polarity control release from solid lipid nanoparticles

    Jensen, L. B., Magnusson, E., Gunnarsson, L., Vermehren, C., Nielsen, H. M. & Petersson, K. 2010 I : Internation Journal of Pharmaceutics. 390, 1, s. 53-60

    Publikation: Forskning - peer reviewTidsskriftartikel

  38. Udgivet

    Cellular and intracellular distribution of recombinant activated factor VII in the rat liver

    Johansen, T. S., Nielsen, H. M., Christensen, E. I. & Appa, R. S. 2010 I : Thrombosis and Haemostasis. 103, 4, s. 860-862

    Publikation: Forskning - peer reviewTidsskriftartikel

  39. Udgivet

    On the temperature dependence of complex formation between chitosan and proteins

    Kasimova, M. R., Valázquez-Campoy, A. & Nielsen, H. M. 2011 I : Biomacromolecules. 12, 7, s. 2534-2543

    Publikation: Forskning - peer reviewTidsskriftartikel

  40. Udgivet

    The unsialylated subpopulation of recombinant activated factor VII binds to the asialo-glycoprotein receptor (ASGPR) on primary rat hepatocytes

    Johansen, T. S., Nielsen, H. M., Christensen, E. I. & Appa, R. S. 2010 I : Thrombosis and Haemostasis. 104, 6, s. 1166-1173

    Publikation: Forskning - peer reviewTidsskriftartikel

  41. Udgivet

    In vivo clearance and metabolism of recombinant activated factor VII (rFVIIa) and its complexes with plasma protease inhibitors in the liver

    Johansen, T. S., Appa, R. S., Christensen, E. I., Ioannou, Y. A., Krogh, T. N., Karpf, D. M. & Nielsen, H. M. 2011 I : Thrombosis Research. 127, 4, s. 356-362

    Publikation: Forskning - peer reviewTidsskriftartikel

  42. Udgivet

    Biodegradable nanocomposite microparticles as drug delivering injectable cell scaffolds

    Wen, Y., Gallego, M. R., Nielsen, L. F., Jørgensen, L., Everland, H., Møller, E. H. & Nielsen, H. M. 2011 I : Journal of Controlled Release. 156, 1, s. 11-20

    Publikation: Forskning - peer reviewTidsskriftartikel

  43. Udgivet

    Issues in development of biodrug delivery systems

    Jørgensen, L., Frøkjær, S. & Nielsen, H. M. 2010 Biodrug delivery systems: fundamentals, applications and clinical development. Morishita, M. & Park, K. (red.). New York: Informa Healthcare, s. 13-25

    Publikation: ForskningBidrag til bog/antologi

  44. Udgivet

    Investigation of the interaction between modified ISCOMs and stratum corneum lipid model systems

    Madsen, H. B., Arboe-Andersen, H. M., Rozlosnik, N., Madsen, F., Ifversen, P., Kasimova, M. R. & Nielsen, H. M. 2010 I : Biochimica et Biophysica Acta - Biomembranes. 1798, s. 1779-1789

    Publikation: Forskning - peer reviewTidsskriftartikel

  45. Udgivet

    Cyclodextrins: efficient biocompatible solubilizing excipients for bromhexine liquid and semi-solid drug delivery systems

    Stojanov, M., Nielsen, H. M. & Larsen, K. L. 2012 I : International Journal of Pharmaceutics. 422, 1-2, s. 349-355

    Publikation: Forskning - peer reviewTidsskriftartikel

  46. Udgivet

    Delivery of dermatan sulfate from polyelectrolyte complex-containing alginate composite microspheres for tissue regeneration

    Wen, Y., Grøndahl, L., Gallego, M. R., Jørgensen, L., Møller, E. H. & Nielsen, H. M. 2012 I : Biomacromolecules. 13, s. 905-917

    Publikation: Forskning - peer reviewTidsskriftartikel

  47. Udgivet

    Structure of immune stimulating complex matrices and immune stimulating complexes in suspension determined by small-angle X-ray scattering

    Pedersen, J. S., Oliveira, C. L. P. D., Madsen, H. B., Arleth, L., Manniche, S., Kirkby, N. & Nielsen, H. M. 2012 I : Biophysical Journal. 102, 10, s. 2372-2380

    Publikation: Forskning - peer reviewTidsskriftartikel

  48. Udgivet

    In vitro placental model optimization for nanoparticle transport studies

    Cartwright, L., Poulsen, M. S., Nielsen, H. M., Pojana, G., Knudsen, L. E., Saunders, M. & Rytting, E. 2012 I : International Journal of Nanomedicine (Online). 7, s. 497-510

    Publikation: Forskning - peer reviewTidsskriftartikel

  49. Udgivet

    Studies towards improved cell-penetrating peptide-promoted macromolecular drug delivery

    Dahlqvist Clausen, J. A., Linderoth, L., Andersen, R. B., Franzyk, H. & Nielsen, H. M. 2010 I : Drug Discovery Today. 15, 23-24, s. 1109 1 s.

    Publikation: ForskningKonferenceabstrakt i tidsskrift

  50. Udgivet

    In vitro penetration properties of solid lipid nanoparticles in intact and barrier-impaired skin

    Jensen, L. B., Petersson, K. & Nielsen, H. M. 2011 I : European Journal of Pharmaceutics and Biopharmaceutics. 79, 1, s. 68-75

    Publikation: Forskning - peer reviewTidsskriftartikel

  51. Udgivet

    Antimicrobial and cell-penetrating properties of penetratin analogs: effect of sequence and secondary structure

    Bahnsen, J. S., Franzyk, H., Sandberg-Schaal, A. & Nielsen, H. M. 2013 I : B B A - Biomembranes. 1828, 2, s. 223-232 10 s.

    Publikation: Forskning - peer reviewTidsskriftartikel

  52. Udgivet

    Solid-phase route to Fmoc-protected cationic amino acid building blocks

    Clausen, J. D., Linderoth, L., Nielsen, H. M. & Franzyk, H. 2012 I : Amino Acids. 43, 4, s. 1633-41 9 s.

    Publikation: Forskning - peer reviewTidsskriftartikel

  53. Udgivet

    Studies towards improved stability and permeability of a cell-penetrating peptide

    Dahlqvist Clausen, J. A., Linderoth, L., Andersen, R. B., Franzyk, H. & Nielsen, H. M. 2010 I : Journal of Peptide Science. 16, s. 148 1 s.

    Publikation: ForskningKonferenceabstrakt i tidsskrift

  54. Udgivet

    Kan nanopartikler bruges i behandlingen af hudsygdomme?

    Jensen, L. B., Knudsen, N. Ø., Foged, C. & Nielsen, H. M. 2010 I : Lægemiddelforskning. 2010, s. 10-12

    Publikation: FormidlingTidsskriftartikel

  55. Udgivet

    Genetisk nanomedicin til behandling af leddegigt

    Foged, C., Jensen, L. B. & Nielsen, H. M. dec. 2011 I : Lægemiddelforskning. s. 7-9

    Publikation: FormidlingTidsskriftartikel

  56. Udgivet

    Gendannelse af ødelagt væv med stamceller

    Møller, E. H., Wen, Y. & Nielsen, H. M. dec. 2011 I : Lægemiddelforskning. 2011, s. 13-15

    Publikation: FormidlingTidsskriftartikel

ID: 1299091