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β2-adrenergic stimulation enhances Ca2+ release and contractile properties of skeletal muscles, and counteracts exercise-induced reductions in Na+/K+-ATPase Vmax in trained men

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Standard

β2-adrenergic stimulation enhances Ca2+ release and contractile properties of skeletal muscles, and counteracts exercise-induced reductions in Na+/K+-ATPase Vmax in trained men. / Hostrup, Morten; Kalsen, A; Ortenblad, N; Juel, Carsten; Mørch, K; Rzeppa, S; Karlsson, S; Backer, Vibeke; Bangsbo, Jens.

I: Journal of Physiology, Bind 592, Nr. 24, 2014, s. 5445-5459.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Hostrup, M, Kalsen, A, Ortenblad, N, Juel, C, Mørch, K, Rzeppa, S, Karlsson, S, Backer, V & Bangsbo, J 2014, 'β2-adrenergic stimulation enhances Ca2+ release and contractile properties of skeletal muscles, and counteracts exercise-induced reductions in Na+/K+-ATPase Vmax in trained men', Journal of Physiology, bind 592, nr. 24, s. 5445-5459. https://doi.org/10.1113/jphysiol.2014.277095

APA

Hostrup, M., Kalsen, A., Ortenblad, N., Juel, C., Mørch, K., Rzeppa, S., ... Bangsbo, J. (2014). β2-adrenergic stimulation enhances Ca2+ release and contractile properties of skeletal muscles, and counteracts exercise-induced reductions in Na+/K+-ATPase Vmax in trained men. Journal of Physiology, 592(24), 5445-5459. https://doi.org/10.1113/jphysiol.2014.277095

Vancouver

Hostrup M, Kalsen A, Ortenblad N, Juel C, Mørch K, Rzeppa S o.a. β2-adrenergic stimulation enhances Ca2+ release and contractile properties of skeletal muscles, and counteracts exercise-induced reductions in Na+/K+-ATPase Vmax in trained men. Journal of Physiology. 2014;592(24):5445-5459. https://doi.org/10.1113/jphysiol.2014.277095

Author

Hostrup, Morten ; Kalsen, A ; Ortenblad, N ; Juel, Carsten ; Mørch, K ; Rzeppa, S ; Karlsson, S ; Backer, Vibeke ; Bangsbo, Jens. / β2-adrenergic stimulation enhances Ca2+ release and contractile properties of skeletal muscles, and counteracts exercise-induced reductions in Na+/K+-ATPase Vmax in trained men. I: Journal of Physiology. 2014 ; Bind 592, Nr. 24. s. 5445-5459.

Bibtex

@article{1d3d89d7f65a4e4db398042bf84efabc,
title = "β2-adrenergic stimulation enhances Ca2+ release and contractile properties of skeletal muscles, and counteracts exercise-induced reductions in Na+/K+-ATPase Vmax in trained men",
abstract = "The aim of the present study was to examine the effect of beta2-adrenergic stimulation on skeletal muscle contractile properties, sarcoplasmic reticulum (SR) rates of Ca(2+) release and uptake, and Na(+)/K(+)-ATPase-activity before and after fatiguing exercise in trained men. The study consisted of two experiments (EXP1, n = 10 M, EXP2, n = 20 M), where beta2-adrenoceptor agonist (terbutaline) or placebo was randomly administered in double-blinded crossover designs. In EXP1, maximal voluntary isometric contraction of m.quadriceps (MVC) was measured, followed by exercise to fatigue at 120{\%} of Vo2max. A muscle biopsy was taken after MVC (non-fatigue) and at time of fatigue. In EXP2, contractile properties of m.quadriceps were measured with electrical stimulations before (non-fatigue) and after two fatiguing 45-s sprints. Non-fatigued MVC was 6±3 and 6±2{\%} higher (P < 0.05) for terbutaline than placebo in EXP1 and EXP2. Furthermore, peak twitch force was 11±7{\%} higher (P < 0.01) for terbutaline than placebo at non-fatigue. After sprints, MVC declined (P < 0.05) to same levels for terbutaline as placebo, whereas peak twitch force was lower (P < 0.05) and half relaxation time prolonged (P < 0.05) with terbutaline. Rates of SR Ca(2+) release and uptake at 400 nM [Ca(2+)] were 15±5 and 14±5{\%} (P < 0.05) higher for terbutaline than placebo at non-fatigue, but declined (P < 0.05) to similar levels at time of fatigue. Na(+)/K(+)-ATPase-activity was unaffected by terbutaline compared with placebo at non-fatigue, but terbutaline counteracted exercise-induced reductions in Vmax at time of fatigue. In conclusion, increased contractile force induced by beta2-adrenergic stimulation is associated with enhanced rate of Ca(2+) release in humans. While beta2-adrenergic stimulation elicits positive inotropic and lusitropic effects of non-fatigued m.quadriceps, these effects are blunted when muscles fatigue. This article is protected by copyright. All rights reserved.",
author = "Morten Hostrup and A Kalsen and N Ortenblad and Carsten Juel and K M{\o}rch and S Rzeppa and S Karlsson and Vibeke Backer and Jens Bangsbo",
note = "CURIS 2014 NEXS 343",
year = "2014",
doi = "10.1113/jphysiol.2014.277095",
language = "English",
volume = "592",
pages = "5445--5459",
journal = "The Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "24",

}

RIS

TY - JOUR

T1 - β2-adrenergic stimulation enhances Ca2+ release and contractile properties of skeletal muscles, and counteracts exercise-induced reductions in Na+/K+-ATPase Vmax in trained men

AU - Hostrup, Morten

AU - Kalsen, A

AU - Ortenblad, N

AU - Juel, Carsten

AU - Mørch, K

AU - Rzeppa, S

AU - Karlsson, S

AU - Backer, Vibeke

AU - Bangsbo, Jens

N1 - CURIS 2014 NEXS 343

PY - 2014

Y1 - 2014

N2 - The aim of the present study was to examine the effect of beta2-adrenergic stimulation on skeletal muscle contractile properties, sarcoplasmic reticulum (SR) rates of Ca(2+) release and uptake, and Na(+)/K(+)-ATPase-activity before and after fatiguing exercise in trained men. The study consisted of two experiments (EXP1, n = 10 M, EXP2, n = 20 M), where beta2-adrenoceptor agonist (terbutaline) or placebo was randomly administered in double-blinded crossover designs. In EXP1, maximal voluntary isometric contraction of m.quadriceps (MVC) was measured, followed by exercise to fatigue at 120% of Vo2max. A muscle biopsy was taken after MVC (non-fatigue) and at time of fatigue. In EXP2, contractile properties of m.quadriceps were measured with electrical stimulations before (non-fatigue) and after two fatiguing 45-s sprints. Non-fatigued MVC was 6±3 and 6±2% higher (P < 0.05) for terbutaline than placebo in EXP1 and EXP2. Furthermore, peak twitch force was 11±7% higher (P < 0.01) for terbutaline than placebo at non-fatigue. After sprints, MVC declined (P < 0.05) to same levels for terbutaline as placebo, whereas peak twitch force was lower (P < 0.05) and half relaxation time prolonged (P < 0.05) with terbutaline. Rates of SR Ca(2+) release and uptake at 400 nM [Ca(2+)] were 15±5 and 14±5% (P < 0.05) higher for terbutaline than placebo at non-fatigue, but declined (P < 0.05) to similar levels at time of fatigue. Na(+)/K(+)-ATPase-activity was unaffected by terbutaline compared with placebo at non-fatigue, but terbutaline counteracted exercise-induced reductions in Vmax at time of fatigue. In conclusion, increased contractile force induced by beta2-adrenergic stimulation is associated with enhanced rate of Ca(2+) release in humans. While beta2-adrenergic stimulation elicits positive inotropic and lusitropic effects of non-fatigued m.quadriceps, these effects are blunted when muscles fatigue. This article is protected by copyright. All rights reserved.

AB - The aim of the present study was to examine the effect of beta2-adrenergic stimulation on skeletal muscle contractile properties, sarcoplasmic reticulum (SR) rates of Ca(2+) release and uptake, and Na(+)/K(+)-ATPase-activity before and after fatiguing exercise in trained men. The study consisted of two experiments (EXP1, n = 10 M, EXP2, n = 20 M), where beta2-adrenoceptor agonist (terbutaline) or placebo was randomly administered in double-blinded crossover designs. In EXP1, maximal voluntary isometric contraction of m.quadriceps (MVC) was measured, followed by exercise to fatigue at 120% of Vo2max. A muscle biopsy was taken after MVC (non-fatigue) and at time of fatigue. In EXP2, contractile properties of m.quadriceps were measured with electrical stimulations before (non-fatigue) and after two fatiguing 45-s sprints. Non-fatigued MVC was 6±3 and 6±2% higher (P < 0.05) for terbutaline than placebo in EXP1 and EXP2. Furthermore, peak twitch force was 11±7% higher (P < 0.01) for terbutaline than placebo at non-fatigue. After sprints, MVC declined (P < 0.05) to same levels for terbutaline as placebo, whereas peak twitch force was lower (P < 0.05) and half relaxation time prolonged (P < 0.05) with terbutaline. Rates of SR Ca(2+) release and uptake at 400 nM [Ca(2+)] were 15±5 and 14±5% (P < 0.05) higher for terbutaline than placebo at non-fatigue, but declined (P < 0.05) to similar levels at time of fatigue. Na(+)/K(+)-ATPase-activity was unaffected by terbutaline compared with placebo at non-fatigue, but terbutaline counteracted exercise-induced reductions in Vmax at time of fatigue. In conclusion, increased contractile force induced by beta2-adrenergic stimulation is associated with enhanced rate of Ca(2+) release in humans. While beta2-adrenergic stimulation elicits positive inotropic and lusitropic effects of non-fatigued m.quadriceps, these effects are blunted when muscles fatigue. This article is protected by copyright. All rights reserved.

U2 - 10.1113/jphysiol.2014.277095

DO - 10.1113/jphysiol.2014.277095

M3 - Journal article

C2 - 25344552

VL - 592

SP - 5445

EP - 5459

JO - The Journal of Physiology

JF - The Journal of Physiology

SN - 0022-3751

IS - 24

ER -

ID: 127251427