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3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and Electron Tomography

Publikation: Bidrag til tidsskriftTidsskriftartikelForskning

Transmission electron microscopy serves as a valuable tool for synaptic structure-function analyses aimed at identifying morphological features or modifications associated with specific developmental stages or dysfunctional synaptic states. By utilizing cryo-preparation techniques to minimize the introduction of structural artifacts during sample preparation, and electron tomography to reconstruct the 3D ultrastructural architecture of a synapse, the spatial organization and morphological properties of synaptic organelles and subcompartments can be quantified with unparalleled precision. In this chapter, we present an experimental approach combining organotypic slice culture, high-pressure freezing, automated freeze-substitution, and electron tomography to investigate spatial relationships between synaptic vesicles and active zone release sites in synapses from lethal mouse mutants.

OriginalsprogEngelsk
TidsskriftMethods in molecular biology (Clifton, N.J.)
Vol/bind1538
Sider (fra-til)215-231
Antal sider17
ISSN1064-3745
DOI
StatusUdgivet - 2017
Eksternt udgivetJa

ID: 237697866