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64Cu loaded liposomes as positron emission tomography imaging agents

Publikation: Bidrag til tidsskriftTidsskriftartikel

  • Anncatrine Luisa Petersen
  • Binderup, Tina
  • Palle Rasmussen
  • Jonas Rosager Henriksen
  • Dennis Ringkjøbing Elema
  • Kjær, Andreas
  • Thomas Lars Andresen
  • Anncatrine Luisa Petersen
  • Tina Binderup
  • Palle Damkjær Rasmussen
  • Jonas Rosager Henriksen
  • Dennis Ringkjøbing Elema
  • Kjær, Andreas
  • Thomas Lars Andresen
We have developed a highly efficient method for utilizing liposomes as imaging agents for positron emission tomography (PET) giving high resolution images and allowing direct quantification of tissue distribution and blood clearance. Our approach is based on remote loading of a copper-radionuclide ((64)Cu) using a new ionophore, 2-hydroxyquinoline, to carry (64)Cu(II) across the membrane of preformed liposomes and deliver it to an encapsulated copper-chelator. Using this ionophore we achieved very efficient loading (95.5 ± 1.6%) and retention stability (>99%), which makes the (64)Cu-liposomes highly applicable as PET imaging agents. We show the utility of the (64)Cu-liposomes for quantitative in vivo imaging of healthy and tumor-bearing mice using PET. This remote loading method is a powerful tool for characterizing the in vivo performance of liposome based nanomedicine, and has great potential in diagnostic and therapeutic applications.
OriginalsprogEngelsk
TidsskriftBiomaterials
Vol/bind32
Udgave nummer9
Sider (fra-til)2334-41
Antal sider8
ISSN0142-9612
DOI
StatusUdgivet - 2011

ID: 33790721