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A combined proteomics and metabolomics approach to assess the effects of gold nanoparticles In vitro

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A combined proteomics and metabolomics approach to assess the effects of gold nanoparticles In vitro. / Gioria, Sabrina; Lobo Vicente, Joana; Barboro, Paola; La Spina, Rita; Tomasi, Giorgio; Urbán, Patricia; Kinsner-Ovaskainen, Agnieszka; Rossi, François; Chassaigne, Hubert.

I: Nanotoxicology, Bind 10, Nr. 6, 2016, s. 736-748.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Gioria, S, Lobo Vicente, J, Barboro, P, La Spina, R, Tomasi, G, Urbán, P, Kinsner-Ovaskainen, A, Rossi, F & Chassaigne, H 2016, 'A combined proteomics and metabolomics approach to assess the effects of gold nanoparticles In vitro', Nanotoxicology, bind 10, nr. 6, s. 736-748. https://doi.org/10.3109/17435390.2015.1121412

APA

Gioria, S., Lobo Vicente, J., Barboro, P., La Spina, R., Tomasi, G., Urbán, P., ... Chassaigne, H. (2016). A combined proteomics and metabolomics approach to assess the effects of gold nanoparticles In vitro. Nanotoxicology, 10(6), 736-748. https://doi.org/10.3109/17435390.2015.1121412

Vancouver

Gioria S, Lobo Vicente J, Barboro P, La Spina R, Tomasi G, Urbán P o.a. A combined proteomics and metabolomics approach to assess the effects of gold nanoparticles In vitro. Nanotoxicology. 2016;10(6):736-748. https://doi.org/10.3109/17435390.2015.1121412

Author

Gioria, Sabrina ; Lobo Vicente, Joana ; Barboro, Paola ; La Spina, Rita ; Tomasi, Giorgio ; Urbán, Patricia ; Kinsner-Ovaskainen, Agnieszka ; Rossi, François ; Chassaigne, Hubert. / A combined proteomics and metabolomics approach to assess the effects of gold nanoparticles In vitro. I: Nanotoxicology. 2016 ; Bind 10, Nr. 6. s. 736-748.

Bibtex

@article{51b4654fdf764970bb305787c8be1a18,
title = "A combined proteomics and metabolomics approach to assess the effects of gold nanoparticles In vitro",
abstract = "Omics technologies, such as proteomics or metabolomics, have to date been applied in the field of nanomaterial safety assessment to a limited extent. To address this dearth, we developed an integrated approach combining the two techniques to study the effects of two sizes, 5 and 30 nm, of gold nanoparticles (AuNPs) in Caco-2 cells. We observed differences in cells exposed for 72 h to each size of AuNPs: 61 responsive (up/down-regulated) proteins were identified and 35 metabolites in the cell extract tentatively annotated. Several altered biological pathways were highlighted by integrating the obtained multi-omics data with bioinformatic tools. This provided a unique set of molecular information on the effects of nanomaterials at cellular level. This information was supported by complementary data obtained by immunochemistry, microscopic analysis and multiplexed assays. A part from increasing our knowledge on how the cellular processes and pathways are affected by nanomaterials (NMs), these findings could be used to identify specific biomarkers of toxicity or to support the safe-by-design concept in the development of new nanomedicines.",
author = "Sabrina Gioria and {Lobo Vicente}, Joana and Paola Barboro and {La Spina}, Rita and Giorgio Tomasi and Patricia Urb{\'a}n and Agnieszka Kinsner-Ovaskainen and Fran{\cc}ois Rossi and Hubert Chassaigne",
year = "2016",
doi = "10.3109/17435390.2015.1121412",
language = "English",
volume = "10",
pages = "736--748",
journal = "Nanotoxicology",
issn = "1743-5390",
publisher = "Informa Healthcare",
number = "6",

}

RIS

TY - JOUR

T1 - A combined proteomics and metabolomics approach to assess the effects of gold nanoparticles In vitro

AU - Gioria, Sabrina

AU - Lobo Vicente, Joana

AU - Barboro, Paola

AU - La Spina, Rita

AU - Tomasi, Giorgio

AU - Urbán, Patricia

AU - Kinsner-Ovaskainen, Agnieszka

AU - Rossi, François

AU - Chassaigne, Hubert

PY - 2016

Y1 - 2016

N2 - Omics technologies, such as proteomics or metabolomics, have to date been applied in the field of nanomaterial safety assessment to a limited extent. To address this dearth, we developed an integrated approach combining the two techniques to study the effects of two sizes, 5 and 30 nm, of gold nanoparticles (AuNPs) in Caco-2 cells. We observed differences in cells exposed for 72 h to each size of AuNPs: 61 responsive (up/down-regulated) proteins were identified and 35 metabolites in the cell extract tentatively annotated. Several altered biological pathways were highlighted by integrating the obtained multi-omics data with bioinformatic tools. This provided a unique set of molecular information on the effects of nanomaterials at cellular level. This information was supported by complementary data obtained by immunochemistry, microscopic analysis and multiplexed assays. A part from increasing our knowledge on how the cellular processes and pathways are affected by nanomaterials (NMs), these findings could be used to identify specific biomarkers of toxicity or to support the safe-by-design concept in the development of new nanomedicines.

AB - Omics technologies, such as proteomics or metabolomics, have to date been applied in the field of nanomaterial safety assessment to a limited extent. To address this dearth, we developed an integrated approach combining the two techniques to study the effects of two sizes, 5 and 30 nm, of gold nanoparticles (AuNPs) in Caco-2 cells. We observed differences in cells exposed for 72 h to each size of AuNPs: 61 responsive (up/down-regulated) proteins were identified and 35 metabolites in the cell extract tentatively annotated. Several altered biological pathways were highlighted by integrating the obtained multi-omics data with bioinformatic tools. This provided a unique set of molecular information on the effects of nanomaterials at cellular level. This information was supported by complementary data obtained by immunochemistry, microscopic analysis and multiplexed assays. A part from increasing our knowledge on how the cellular processes and pathways are affected by nanomaterials (NMs), these findings could be used to identify specific biomarkers of toxicity or to support the safe-by-design concept in the development of new nanomedicines.

U2 - 10.3109/17435390.2015.1121412

DO - 10.3109/17435390.2015.1121412

M3 - Journal article

C2 - 26647645

VL - 10

SP - 736

EP - 748

JO - Nanotoxicology

JF - Nanotoxicology

SN - 1743-5390

IS - 6

ER -

ID: 155551824