Forskning ved Københavns Universitet - Københavns Universitet

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A Generic Method for Fungal Spore Detection: The use of a monoclonal antibody and surface plasmon resonance

Publikation: KonferencebidragPosterForskning

  • Peter Durand Skottrup
  • Stephen Hearty
  • Hanne Frøkiær
  • Paul Leonard
  • Jørn Hejgaard
  • Richard O'Kennedy
  • Mogens Nicolaisen
  • Annemarie Fejer Justesen
This study describes a biosensing principle for detection of fungal spores using surface plasmon resonance (SPR). The approach involves the use of a monoclonal antibody (mab) and a SPR sensor for label-free detection of the model organism Puccinia striiformis f.sp. tritici (Pst) a biotrophic fungus causing wheat yellow rust. We have developed mabs towards intact whole spores and used a subtractive inhibition format for detection of spores in solution. The antibody was incubated with different spore concentrations and the remaining free antibody was quantified using a BIAcore® 3000 sensor. Decreasing binding of mab to the sensor surface was observed as the Pst urediniospore concentration was increased. The detection range for the assay was 1.7 x 106 – 5.3 x 104 spores/ml. This study describes the first use of SPR for detection of fungal spores and the generic principle has the potential to be used in detection of any spore species and could be implemented in future on-site biosensors.
OriginalsprogEngelsk
Publikationsdato2005
StatusUdgivet - 2005
Begivenhed3rd annual iNANO meeting - Abeltoft, Danmark
Varighed: 19 jan. 2005 → …

Konference

Konference3rd annual iNANO meeting
LandDanmark
ByAbeltoft
Periode19/01/2005 → …

Bibliografisk note

Presented at: 3rd annual iNANO meeting : Ebeltoft, Denmark, 2005

ID: 33238520