Forskning ved Københavns Universitet - Københavns Universitet

Forside

A method for production and determination of histamine releasing activity from human peripheral blood mononuclear cells

Publikation: Forskning - peer reviewTidsskriftartikel

G T Kampen, L K Poulsen, C M Reimert, P S Skov

Histamine releasing factors, i.e. cytokines capable of inducing histamine release from basophils or mast cells, have been suggested to be involved in the pathogenesis of, for example, allergic late-phase reactions. Here we describe a controlled method for production and determination of histamine releasing activity (HRA) from human peripheral blood mononuclear cells (MNC). MNC were incubated with concanavalin A (Con A) for 2 h and cultured for another 40 h in fresh serum free medium. The culture supernatants were concentrated 19-25 fold by ultrafiltration (molecular weight cut-off: 3000 Da). The preparations of HRA induced dose- and Ca2+-dependent histamine release from leukocytes. Supernatants of parallel cultures of unstimulated MNC did not induce histamine release. The HRA was neither due to exogenous histamine releasing compounds (e.g. Con A) nor to residual histamine in the preparations of HRA. The kinetics of HRA induced histamine release (half-maximal release after > 40 min) were slower and more protracted than those of anti-IgE induced histamine release. However, based on a comparison between HRA induced histamine release from leukocytes and purified (97%) basophils, this did not appear to be due to an indirect effect on the basophils. Finally, neither the production of nor the response to HRA was dependent on the allergic status of the donor.

OriginalsprogEngelsk
TidsskriftJournal of Immunological Methods
Vol/bind210
Tidsskriftsnummer2
Sider (fra-til)185-93
Antal sider9
ISSN0022-1759
StatusUdgivet - 29 dec. 1997

ID: 169715886