Forskning ved Københavns Universitet - Københavns Universitet

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A modular luminescence lifetime imaging system for mapping oxygen distribution in biological samples

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  • Gerhard Holst
  • Oliver Kohls
  • Ingo Klimant
  • Bettina König
  • Kuhl, Michael
  • Thomas Richter

We developed a new modular luminescence lifetime imaging system (MOLLI), that enables the imaging of luminescence lifetimes in the range of 1 μs to 1 s. The system can easily be adapted to different experimental applications. The central parts of the system are a recently released CCD-camera with a fast electronic shutter and gated LED (light emitting diode) or Xe excitation light sources. A personal computer controls the gating and image acquisition via a pulse delay generator. Here we present the new imaging system and give examples of its performance when used for measuring two-dimensional oxygen distributions with planar optodes. Furthermore, future applications of the system in biology are discussed.

OriginalsprogEngelsk
TidsskriftSensors and Actuators, B: Chemical
Vol/bind51
Udgave nummer1-3
Sider (fra-til)163-170
Antal sider8
ISSN0925-4005
DOI
StatusUdgivet - 31 aug. 1998

ID: 201682581