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A Novel and Direct Metamobilome Approach improves the Detection of Larger-sized Circular Elements across Kingdoms

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Standard

A Novel and Direct Metamobilome Approach improves the Detection of Larger-sized Circular Elements across Kingdoms. / Alanin, Katrine Skov; Jørgensen, Tue Sparholt; Browne, Patrick; Petersen, Bent; Riber, Leise; Kot, Witold Piotr; Hansen, Lars Hestbjerg.

I: bioRxiv, 2020.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskning

Harvard

Alanin, KS, Jørgensen, TS, Browne, P, Petersen, B, Riber, L, Kot, WP & Hansen, LH 2020, 'A Novel and Direct Metamobilome Approach improves the Detection of Larger-sized Circular Elements across Kingdoms', bioRxiv. https://doi.org/10.1101/761098

APA

Alanin, K. S., Jørgensen, T. S., Browne, P., Petersen, B., Riber, L., Kot, W. P., & Hansen, L. H. (2020). A Novel and Direct Metamobilome Approach improves the Detection of Larger-sized Circular Elements across Kingdoms. Manuskript afsendt til publicering. https://doi.org/10.1101/761098

Vancouver

Alanin KS, Jørgensen TS, Browne P, Petersen B, Riber L, Kot WP o.a. A Novel and Direct Metamobilome Approach improves the Detection of Larger-sized Circular Elements across Kingdoms. bioRxiv. 2020. 761098. https://doi.org/10.1101/761098

Author

Alanin, Katrine Skov ; Jørgensen, Tue Sparholt ; Browne, Patrick ; Petersen, Bent ; Riber, Leise ; Kot, Witold Piotr ; Hansen, Lars Hestbjerg. / A Novel and Direct Metamobilome Approach improves the Detection of Larger-sized Circular Elements across Kingdoms. I: bioRxiv. 2020.

Bibtex

@article{7fdd6b334afc43a7a7fdb688f5a88347,
title = "A Novel and Direct Metamobilome Approach improves the Detection of Larger-sized Circular Elements across Kingdoms",
abstract = "Mobile genetic elements (MGEs) are instrumental in natural prokaryotic genome editing, permitting genome plasticity and allowing microbes to accumulate immense genetic diversity. MGEs include DNA elements such as plasmids, transposons and Insertion Sequences (IS-elements), as well as bacteriophages (phages), and they serve as a vast communal gene pool. These mobile DNA elements represent a human health risk as they can add new traits, such as antibiotic resistance or virulence, to a bacterial strain. Sequencing libraries targeting circular MGEs, referred to as mobilomes, allows the expansion of our current understanding of the mechanisms behind the mobility, prevalence and content of these elements. However, mobilomes are not studied to the same extent as bacterial genomes, partly because of methodological biases arising from multiple displacement amplification (MDA), often used in previous mobilome publications. In this study, we show that MDA is detrimental for the detection of larger-sized plasmids if small plasmids are present by comparing the abundances of reads mapping to plasmids in a wastewater sample spiked with a mock community of selected plasmids with and without MDA. Furthermore, we show that it is possible to produce samples consisting almost exclusively of circular MGEs and obtain a catalog of larger, complete, circular MGEs from complex samples without the use of MDA.",
author = "Alanin, {Katrine Skov} and J{\o}rgensen, {Tue Sparholt} and Patrick Browne and Bent Petersen and Leise Riber and Kot, {Witold Piotr} and Hansen, {Lars Hestbjerg}",
year = "2020",
doi = "10.1101/761098",
language = "English",
journal = "bioRxiv",

}

RIS

TY - JOUR

T1 - A Novel and Direct Metamobilome Approach improves the Detection of Larger-sized Circular Elements across Kingdoms

AU - Alanin, Katrine Skov

AU - Jørgensen, Tue Sparholt

AU - Browne, Patrick

AU - Petersen, Bent

AU - Riber, Leise

AU - Kot, Witold Piotr

AU - Hansen, Lars Hestbjerg

PY - 2020

Y1 - 2020

N2 - Mobile genetic elements (MGEs) are instrumental in natural prokaryotic genome editing, permitting genome plasticity and allowing microbes to accumulate immense genetic diversity. MGEs include DNA elements such as plasmids, transposons and Insertion Sequences (IS-elements), as well as bacteriophages (phages), and they serve as a vast communal gene pool. These mobile DNA elements represent a human health risk as they can add new traits, such as antibiotic resistance or virulence, to a bacterial strain. Sequencing libraries targeting circular MGEs, referred to as mobilomes, allows the expansion of our current understanding of the mechanisms behind the mobility, prevalence and content of these elements. However, mobilomes are not studied to the same extent as bacterial genomes, partly because of methodological biases arising from multiple displacement amplification (MDA), often used in previous mobilome publications. In this study, we show that MDA is detrimental for the detection of larger-sized plasmids if small plasmids are present by comparing the abundances of reads mapping to plasmids in a wastewater sample spiked with a mock community of selected plasmids with and without MDA. Furthermore, we show that it is possible to produce samples consisting almost exclusively of circular MGEs and obtain a catalog of larger, complete, circular MGEs from complex samples without the use of MDA.

AB - Mobile genetic elements (MGEs) are instrumental in natural prokaryotic genome editing, permitting genome plasticity and allowing microbes to accumulate immense genetic diversity. MGEs include DNA elements such as plasmids, transposons and Insertion Sequences (IS-elements), as well as bacteriophages (phages), and they serve as a vast communal gene pool. These mobile DNA elements represent a human health risk as they can add new traits, such as antibiotic resistance or virulence, to a bacterial strain. Sequencing libraries targeting circular MGEs, referred to as mobilomes, allows the expansion of our current understanding of the mechanisms behind the mobility, prevalence and content of these elements. However, mobilomes are not studied to the same extent as bacterial genomes, partly because of methodological biases arising from multiple displacement amplification (MDA), often used in previous mobilome publications. In this study, we show that MDA is detrimental for the detection of larger-sized plasmids if small plasmids are present by comparing the abundances of reads mapping to plasmids in a wastewater sample spiked with a mock community of selected plasmids with and without MDA. Furthermore, we show that it is possible to produce samples consisting almost exclusively of circular MGEs and obtain a catalog of larger, complete, circular MGEs from complex samples without the use of MDA.

U2 - 10.1101/761098

DO - 10.1101/761098

M3 - Journal article

JO - bioRxiv

JF - bioRxiv

M1 - 761098

ER -

ID: 227842312