Forskning ved Københavns Universitet - Københavns Universitet

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A novel, specific pro-urokinase complex on monocyte-like cells, detected by transglutaminase-catalyzed cross-linking

Publikation: Forskning - peer reviewTidsskriftartikel

N Behrendt, E Rønne, K Danø

Radiolabeled pro-urokinase plasminogen activator (pro-uPA) was cross-linked to a specific protein on the surface of human monocyte-like U937 cells in a reaction catalyzed by tissue transglutaminase. The conjugate formed with this unknown component had a much higher molecular weight (apparent M(r) 250,000-300,000) than the complex of pro-uPA and the urokinase plasminogen activator receptor (uPAR). There was a strong preference for the pro-form of uPA. The conjugate was recognized by antibodies against uPA but not by anti-uPAR antibodies. Nevertheless, the blocking of uPAR with a monoclonal antibody abolished the formation of the conjugate, thus showing a role of uPAR in this process.

OriginalsprogEngelsk
TidsskriftFEBS letters
Vol/bind336
Tidsskriftsnummer3
Sider (fra-til)394-6
Antal sider3
ISSN0014-5793
StatusUdgivet - 28 dec. 1993
Eksternt udgivetJa

ID: 180823599