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A novel, specific pro-urokinase complex on monocyte-like cells, detected by transglutaminase-catalyzed cross-linking

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Standard

A novel, specific pro-urokinase complex on monocyte-like cells, detected by transglutaminase-catalyzed cross-linking. / Behrendt, N; Rønne, E; Danø, K.

I: FEBS letters, Bind 336, Nr. 3, 28.12.1993, s. 394-6.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Behrendt, N, Rønne, E & Danø, K 1993, 'A novel, specific pro-urokinase complex on monocyte-like cells, detected by transglutaminase-catalyzed cross-linking', FEBS letters, bind 336, nr. 3, s. 394-6.

APA

Behrendt, N., Rønne, E., & Danø, K. (1993). A novel, specific pro-urokinase complex on monocyte-like cells, detected by transglutaminase-catalyzed cross-linking. FEBS letters, 336(3), 394-6.

Vancouver

Behrendt N, Rønne E, Danø K. A novel, specific pro-urokinase complex on monocyte-like cells, detected by transglutaminase-catalyzed cross-linking. FEBS letters. 1993 dec 28;336(3):394-6.

Author

Behrendt, N ; Rønne, E ; Danø, K. / A novel, specific pro-urokinase complex on monocyte-like cells, detected by transglutaminase-catalyzed cross-linking. I: FEBS letters. 1993 ; Bind 336, Nr. 3. s. 394-6.

Bibtex

@article{cafad4aaef3342df9603e7fa6482f9c9,
title = "A novel, specific pro-urokinase complex on monocyte-like cells, detected by transglutaminase-catalyzed cross-linking",
abstract = "Radiolabeled pro-urokinase plasminogen activator (pro-uPA) was cross-linked to a specific protein on the surface of human monocyte-like U937 cells in a reaction catalyzed by tissue transglutaminase. The conjugate formed with this unknown component had a much higher molecular weight (apparent M(r) 250,000-300,000) than the complex of pro-uPA and the urokinase plasminogen activator receptor (uPAR). There was a strong preference for the pro-form of uPA. The conjugate was recognized by antibodies against uPA but not by anti-uPAR antibodies. Nevertheless, the blocking of uPAR with a monoclonal antibody abolished the formation of the conjugate, thus showing a role of uPAR in this process.",
keywords = "Cell Line, Cell Membrane, Cross-Linking Reagents, Electrophoresis, Polyacrylamide Gel, Humans, Lymphoma, Large B-Cell, Diffuse, Molecular Weight, Monocytes, Receptors, Cell Surface, Receptors, Urokinase Plasminogen Activator, Recombinant Proteins, Transglutaminases, Tumor Cells, Cultured, Urokinase-Type Plasminogen Activator, Journal Article, Research Support, Non-U.S. Gov't",
author = "N Behrendt and E R{\o}nne and K Dan{\o}",
year = "1993",
month = "12",
day = "28",
language = "English",
volume = "336",
pages = "394--6",
journal = "F E B S Letters",
issn = "0014-5793",
publisher = "JohnWiley & Sons Ltd",
number = "3",

}

RIS

TY - JOUR

T1 - A novel, specific pro-urokinase complex on monocyte-like cells, detected by transglutaminase-catalyzed cross-linking

AU - Behrendt, N

AU - Rønne, E

AU - Danø, K

PY - 1993/12/28

Y1 - 1993/12/28

N2 - Radiolabeled pro-urokinase plasminogen activator (pro-uPA) was cross-linked to a specific protein on the surface of human monocyte-like U937 cells in a reaction catalyzed by tissue transglutaminase. The conjugate formed with this unknown component had a much higher molecular weight (apparent M(r) 250,000-300,000) than the complex of pro-uPA and the urokinase plasminogen activator receptor (uPAR). There was a strong preference for the pro-form of uPA. The conjugate was recognized by antibodies against uPA but not by anti-uPAR antibodies. Nevertheless, the blocking of uPAR with a monoclonal antibody abolished the formation of the conjugate, thus showing a role of uPAR in this process.

AB - Radiolabeled pro-urokinase plasminogen activator (pro-uPA) was cross-linked to a specific protein on the surface of human monocyte-like U937 cells in a reaction catalyzed by tissue transglutaminase. The conjugate formed with this unknown component had a much higher molecular weight (apparent M(r) 250,000-300,000) than the complex of pro-uPA and the urokinase plasminogen activator receptor (uPAR). There was a strong preference for the pro-form of uPA. The conjugate was recognized by antibodies against uPA but not by anti-uPAR antibodies. Nevertheless, the blocking of uPAR with a monoclonal antibody abolished the formation of the conjugate, thus showing a role of uPAR in this process.

KW - Cell Line

KW - Cell Membrane

KW - Cross-Linking Reagents

KW - Electrophoresis, Polyacrylamide Gel

KW - Humans

KW - Lymphoma, Large B-Cell, Diffuse

KW - Molecular Weight

KW - Monocytes

KW - Receptors, Cell Surface

KW - Receptors, Urokinase Plasminogen Activator

KW - Recombinant Proteins

KW - Transglutaminases

KW - Tumor Cells, Cultured

KW - Urokinase-Type Plasminogen Activator

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

C2 - 7904248

VL - 336

SP - 394

EP - 396

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 3

ER -

ID: 180823599