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A rapid polymerase chain reaction (PCR)-based assay for the identification of Listeria monocytogenes in food samples

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A rapid and simple assay has been developed which allows specific detection of Listeria monocytogenes within 3.5 h in cultures prepared from suspect food samples and propagated 48 h in selective medium. The assay is based on PCR technology, and uses a specific primer set derived from sequences located down-stream of the hlyA gene. The specificity of the primer set was confirmed by testing 115 L. monocytogenes, 14 L. innocua, 5 L. seeligeri and 4 L. ivanovii isolates. The assay was compared to standard microbiological tests and gave identical results for 83 food samples, including 32 positives. These field trials indicate that the assay developed provides an alternative detection system for L. monocytogenes in foods, which can be used by the food industry.

OriginalsprogEngelsk
TidsskriftInternational Journal of Food Microbiology
Vol/bind14
Udgave nummer2
Sider (fra-til)145-151
Antal sider7
ISSN0168-1605
DOI
StatusUdgivet - nov. 1991

ID: 257697963