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A response calculus for immobilized T cell receptor ligands

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Standard

A response calculus for immobilized T cell receptor ligands. / Andersen, P S; Menné, C; Mariuzza, R A; Geisler, C; Karjalainen, K.

I: Journal of Biological Chemistry, Bind 276, Nr. 52, 2001, s. 49125-32.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Andersen, PS, Menné, C, Mariuzza, RA, Geisler, C & Karjalainen, K 2001, 'A response calculus for immobilized T cell receptor ligands', Journal of Biological Chemistry, bind 276, nr. 52, s. 49125-32. https://doi.org/10.1074/jbc.M109396200

APA

Andersen, P. S., Menné, C., Mariuzza, R. A., Geisler, C., & Karjalainen, K. (2001). A response calculus for immobilized T cell receptor ligands. Journal of Biological Chemistry, 276(52), 49125-32. https://doi.org/10.1074/jbc.M109396200

Vancouver

Andersen PS, Menné C, Mariuzza RA, Geisler C, Karjalainen K. A response calculus for immobilized T cell receptor ligands. Journal of Biological Chemistry. 2001;276(52):49125-32. https://doi.org/10.1074/jbc.M109396200

Author

Andersen, P S ; Menné, C ; Mariuzza, R A ; Geisler, C ; Karjalainen, K. / A response calculus for immobilized T cell receptor ligands. I: Journal of Biological Chemistry. 2001 ; Bind 276, Nr. 52. s. 49125-32.

Bibtex

@article{4bbe9f70b0a111ddb538000ea68e967b,
title = "A response calculus for immobilized T cell receptor ligands",
abstract = "To address the molecular mechanism of T cell receptor (TCR) signaling, we have formulated a model for T cell activation, termed the 2D-affinity model, in which the density of TCR on the T cell surface, the density of ligand on the presenting surface, and their corresponding two-dimensional affinity determine the level of T cell activation. When fitted to T cell responses against purified ligands immobilized on plastic surfaces, the 2D-affinity model adequately simulated changes in cellular activation as a result of varying ligand affinity and ligand density. These observations further demonstrated the importance of receptor cross-linking density in determining TCR signaling. Moreover, it was found that the functional two-dimensional affinity of TCR ligands was affected by the chemical composition of the ligand-presenting surface. This makes it possible that cell-bound TCR ligands, despite their low affinity in solution, are of optimal two-dimensional affinity thereby allowing effective TCR binding under physiological conditions, i.e. at low ligand densities in cellular interfaces.",
author = "Andersen, {P S} and C Menn{\'e} and Mariuzza, {R A} and C Geisler and K Karjalainen",
note = "Keywords: Antibodies; Genes, Reporter; Ligands; Lymphocyte Activation; Mathematics; Models, Immunological; Protein Binding; Receptors, Antigen, T-Cell; Recombinant Fusion Proteins; Signal Transduction; Superantigens; T-Lymphocytes",
year = "2001",
doi = "10.1074/jbc.M109396200",
language = "English",
volume = "276",
pages = "49125--32",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "52",

}

RIS

TY - JOUR

T1 - A response calculus for immobilized T cell receptor ligands

AU - Andersen, P S

AU - Menné, C

AU - Mariuzza, R A

AU - Geisler, C

AU - Karjalainen, K

N1 - Keywords: Antibodies; Genes, Reporter; Ligands; Lymphocyte Activation; Mathematics; Models, Immunological; Protein Binding; Receptors, Antigen, T-Cell; Recombinant Fusion Proteins; Signal Transduction; Superantigens; T-Lymphocytes

PY - 2001

Y1 - 2001

N2 - To address the molecular mechanism of T cell receptor (TCR) signaling, we have formulated a model for T cell activation, termed the 2D-affinity model, in which the density of TCR on the T cell surface, the density of ligand on the presenting surface, and their corresponding two-dimensional affinity determine the level of T cell activation. When fitted to T cell responses against purified ligands immobilized on plastic surfaces, the 2D-affinity model adequately simulated changes in cellular activation as a result of varying ligand affinity and ligand density. These observations further demonstrated the importance of receptor cross-linking density in determining TCR signaling. Moreover, it was found that the functional two-dimensional affinity of TCR ligands was affected by the chemical composition of the ligand-presenting surface. This makes it possible that cell-bound TCR ligands, despite their low affinity in solution, are of optimal two-dimensional affinity thereby allowing effective TCR binding under physiological conditions, i.e. at low ligand densities in cellular interfaces.

AB - To address the molecular mechanism of T cell receptor (TCR) signaling, we have formulated a model for T cell activation, termed the 2D-affinity model, in which the density of TCR on the T cell surface, the density of ligand on the presenting surface, and their corresponding two-dimensional affinity determine the level of T cell activation. When fitted to T cell responses against purified ligands immobilized on plastic surfaces, the 2D-affinity model adequately simulated changes in cellular activation as a result of varying ligand affinity and ligand density. These observations further demonstrated the importance of receptor cross-linking density in determining TCR signaling. Moreover, it was found that the functional two-dimensional affinity of TCR ligands was affected by the chemical composition of the ligand-presenting surface. This makes it possible that cell-bound TCR ligands, despite their low affinity in solution, are of optimal two-dimensional affinity thereby allowing effective TCR binding under physiological conditions, i.e. at low ligand densities in cellular interfaces.

U2 - 10.1074/jbc.M109396200

DO - 10.1074/jbc.M109396200

M3 - Journal article

C2 - 11592972

VL - 276

SP - 49125

EP - 49132

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 52

ER -

ID: 8544701