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A simple method for validation and verification of pipettes mounted on automated liquid handlers

Publikation: KonferencebidragPosterForskning

Standard

A simple method for validation and verification of pipettes mounted on automated liquid handlers. / Stangegaard, Michael; Hansen, Anders Johannes; Frøslev, Tobias Guldberg; Morling, Niels.

2009. Poster session præsenteret ved LabAutomation2009, Palm Springs 2009, USA.

Publikation: KonferencebidragPosterForskning

Harvard

Stangegaard, M, Hansen, AJ, Frøslev, TG & Morling, N 2009, 'A simple method for validation and verification of pipettes mounted on automated liquid handlers' LabAutomation2009, Palm Springs 2009, USA, 25/01/2009 - 28/01/2009, .

APA

Stangegaard, M., Hansen, A. J., Frøslev, T. G., & Morling, N. (2009). A simple method for validation and verification of pipettes mounted on automated liquid handlers. Poster session præsenteret ved LabAutomation2009, Palm Springs 2009, USA.

Vancouver

Stangegaard M, Hansen AJ, Frøslev TG, Morling N. A simple method for validation and verification of pipettes mounted on automated liquid handlers. 2009. Poster session præsenteret ved LabAutomation2009, Palm Springs 2009, USA.

Author

Stangegaard, Michael ; Hansen, Anders Johannes ; Frøslev, Tobias Guldberg ; Morling, Niels. / A simple method for validation and verification of pipettes mounted on automated liquid handlers. Poster session præsenteret ved LabAutomation2009, Palm Springs 2009, USA.

Bibtex

@conference{a051d9a0af4011debc73000ea68e967b,
title = "A simple method for validation and verification of pipettes mounted on automated liquid handlers",
abstract = " We have implemented a simple method for validation and verification of the performance of pipettes mounted on automated liquid handlers as necessary for laboratories accredited under ISO 17025. An 8-step serial dilution of Orange G was prepared in quadruplicates in a flat bottom 96-well microtiter plate (BD Falcon) manually by means of calibrated pipettes. Each pipette of the liquid handler (1 up to 8) dispensed a selected volume (1 to 200µl) of Orange G 8 times into the wells of the microtiter plate. All wells contained a total of 200µL liquid. The optical density (OD) was read at 490 nm, and the dispensed volume of each pipette was calculated based on a plot of volume and OD of a known set of Orange G dilutions. Finally, the percent inaccuracy ({\%}d) and the imprecision ({\%}CV) of each pipette was calculated. Using pre-defined acceptance criteria, each pipette was then either approved or failed. Failed pipettes were either repaired or the volume deviation was compensated for by applying a calibration curve in the liquid handler software. We have implemented the method on a Sias Xantus, an MWGt TheONYX, three Tecan Freedom EVO, a Biomek NX Span-8 and four Biomek 3000 robots, and the scripts are freely available. In conclusion, we have set up a simple solution for the continuous validation of automated liquid handlers used for accredited work. The method is cheap, simple and easy to use for aqueous solutions but requires a spectrophotometer that can read microtiter plates.",
keywords = "The Faculty of Health and Medical Sciences",
author = "Michael Stangegaard and Hansen, {Anders Johannes} and Fr{\o}slev, {Tobias Guldberg} and Niels Morling",
note = "Titel p{\aa} proceedings: LabAutomation2009 Sider: 134; null ; Conference date: 25-01-2009 Through 28-01-2009",
year = "2009",
language = "English",

}

RIS

TY - CONF

T1 - A simple method for validation and verification of pipettes mounted on automated liquid handlers

AU - Stangegaard, Michael

AU - Hansen, Anders Johannes

AU - Frøslev, Tobias Guldberg

AU - Morling, Niels

N1 - Titel på proceedings: LabAutomation2009 Sider: 134

PY - 2009

Y1 - 2009

N2 -  We have implemented a simple method for validation and verification of the performance of pipettes mounted on automated liquid handlers as necessary for laboratories accredited under ISO 17025. An 8-step serial dilution of Orange G was prepared in quadruplicates in a flat bottom 96-well microtiter plate (BD Falcon) manually by means of calibrated pipettes. Each pipette of the liquid handler (1 up to 8) dispensed a selected volume (1 to 200µl) of Orange G 8 times into the wells of the microtiter plate. All wells contained a total of 200µL liquid. The optical density (OD) was read at 490 nm, and the dispensed volume of each pipette was calculated based on a plot of volume and OD of a known set of Orange G dilutions. Finally, the percent inaccuracy (%d) and the imprecision (%CV) of each pipette was calculated. Using pre-defined acceptance criteria, each pipette was then either approved or failed. Failed pipettes were either repaired or the volume deviation was compensated for by applying a calibration curve in the liquid handler software. We have implemented the method on a Sias Xantus, an MWGt TheONYX, three Tecan Freedom EVO, a Biomek NX Span-8 and four Biomek 3000 robots, and the scripts are freely available. In conclusion, we have set up a simple solution for the continuous validation of automated liquid handlers used for accredited work. The method is cheap, simple and easy to use for aqueous solutions but requires a spectrophotometer that can read microtiter plates.

AB -  We have implemented a simple method for validation and verification of the performance of pipettes mounted on automated liquid handlers as necessary for laboratories accredited under ISO 17025. An 8-step serial dilution of Orange G was prepared in quadruplicates in a flat bottom 96-well microtiter plate (BD Falcon) manually by means of calibrated pipettes. Each pipette of the liquid handler (1 up to 8) dispensed a selected volume (1 to 200µl) of Orange G 8 times into the wells of the microtiter plate. All wells contained a total of 200µL liquid. The optical density (OD) was read at 490 nm, and the dispensed volume of each pipette was calculated based on a plot of volume and OD of a known set of Orange G dilutions. Finally, the percent inaccuracy (%d) and the imprecision (%CV) of each pipette was calculated. Using pre-defined acceptance criteria, each pipette was then either approved or failed. Failed pipettes were either repaired or the volume deviation was compensated for by applying a calibration curve in the liquid handler software. We have implemented the method on a Sias Xantus, an MWGt TheONYX, three Tecan Freedom EVO, a Biomek NX Span-8 and four Biomek 3000 robots, and the scripts are freely available. In conclusion, we have set up a simple solution for the continuous validation of automated liquid handlers used for accredited work. The method is cheap, simple and easy to use for aqueous solutions but requires a spectrophotometer that can read microtiter plates.

KW - The Faculty of Health and Medical Sciences

M3 - Poster

ER -

ID: 14880669