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A Sleeping Beauty DNA transposon-based genetic sensor for functional screening of vitamin D3 analogues

Publikation: Bidrag til tidsskriftTidsskriftartikel

Dokumenter

  • Nicklas Heine Staunstrup
  • Nynne Sharma
  • Rasmus Otkjær Bak
  • Lars Svensson
  • Thomas K. Petersen
  • Lene Aarenstrup
  • Kristiansen, Karsten
  • Lars Bolund
  • Jacob Giehm Mikkelsen
Analogues of vitamin D3 are extensively used in the treatment of various illnesses, such as osteoporosis, inflammatory skin diseases, and cancer. Functional testing of new vitamin D3 analogues and formulations for improved systemic and topical administration is supported by sensitive screening methods that allow a comparative evaluation of drug properties. As a new tool in functional screening of vitamin D3 analogues, we describe a genomically integratable sensor for sensitive drug detection. This system facilitates assessment of the pharmacokinetic and pharmadynamic properties of vitamin D3 analogues. The tri-cistronic genetic sensor encodes a drug-sensoring protein, a reporter protein expressed from an activated sensor-responsive promoter, and a resistance marker.
OriginalsprogEngelsk
TidsskriftB M C Biotechnology
Vol/bind11
Antal sider13
ISSN1472-6750
DOI
StatusUdgivet - 2011

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