Forskning ved Københavns Universitet - Københavns Universitet

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A Surface Plasmon Resonance Immunosensor for Detection of urediniospores from Puccinia striiformis f. sp. tritici

Publikation: KonferencebidragPosterForskning

  • Peter Durand Skottrup
  • Stephen Hearty
  • Hanne Frøkiær
  • Paul Leonard
  • Jørn Hejgaard
  • Richard O'Kennedy
  • Mogens Nicolaisen
  • Annemarie Fejer Justesen
This study describes a generic biosensing principle for detection of fungal spores using surface plasmon resonance (SPR). The approach involves the use of a mouse monoclonal antibody (mAb) and a SPR sensor for label-free detection of the model organism Puccinia striiformis f.sp. tritici (Pst). We developed mAbs towards intact whole spores and used a subtractive inhibition format for detection of spores in solution. Assay conditions was optimised and a detection limit of 3.9 x 103 spores/ml was achieved. We further examined spiked Pst samples in a background of a related spore and found that Pst quantification was possible in this complex matrix. To the best of our knowledge, this study simultaneously represents the first use of SPR technology for fungal spore detection and the first report of a successful biosensor-based detection strategy for Pst
OriginalsprogEngelsk
Publikationsdato2006
StatusUdgivet - 2006
Eksternt udgivetJa
BegivenhedEighth European Conference on optical chemical and biosensors - Tubingen, Tyskland
Varighed: 2 apr. 20065 apr. 2006

Konference

KonferenceEighth European Conference on optical chemical and biosensors
LandTyskland
ByTubingen
Periode02/04/200605/04/2006

ID: 33226602