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A synoviocyte model for osteoarthritis and rheumatoid arthritis: response to Ibuprofen, betamethasone, and ginger extract-a cross-sectional in vitro study

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

A synoviocyte model for osteoarthritis and rheumatoid arthritis : response to Ibuprofen, betamethasone, and ginger extract-a cross-sectional in vitro study. / Ribel-Madsen, Søren; Bartels, Else Marie; Stockmarr, Anders; Borgwardt, Arne; Cornett, Claus; Danneskiold-Samsøe, Bente; Bliddal, Henning.

I: Arthritis, Bind 2012, 2012, s. 505842.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Ribel-Madsen, S, Bartels, EM, Stockmarr, A, Borgwardt, A, Cornett, C, Danneskiold-Samsøe, B & Bliddal, H 2012, 'A synoviocyte model for osteoarthritis and rheumatoid arthritis: response to Ibuprofen, betamethasone, and ginger extract-a cross-sectional in vitro study', Arthritis, bind 2012, s. 505842. https://doi.org/10.1155/2012/505842

APA

Ribel-Madsen, S., Bartels, E. M., Stockmarr, A., Borgwardt, A., Cornett, C., Danneskiold-Samsøe, B., & Bliddal, H. (2012). A synoviocyte model for osteoarthritis and rheumatoid arthritis: response to Ibuprofen, betamethasone, and ginger extract-a cross-sectional in vitro study. Arthritis, 2012, 505842. https://doi.org/10.1155/2012/505842

Vancouver

Ribel-Madsen S, Bartels EM, Stockmarr A, Borgwardt A, Cornett C, Danneskiold-Samsøe B o.a. A synoviocyte model for osteoarthritis and rheumatoid arthritis: response to Ibuprofen, betamethasone, and ginger extract-a cross-sectional in vitro study. Arthritis. 2012;2012:505842. https://doi.org/10.1155/2012/505842

Author

Ribel-Madsen, Søren ; Bartels, Else Marie ; Stockmarr, Anders ; Borgwardt, Arne ; Cornett, Claus ; Danneskiold-Samsøe, Bente ; Bliddal, Henning. / A synoviocyte model for osteoarthritis and rheumatoid arthritis : response to Ibuprofen, betamethasone, and ginger extract-a cross-sectional in vitro study. I: Arthritis. 2012 ; Bind 2012. s. 505842.

Bibtex

@article{7798563da61d4c8bab192fc60a9c9d27,
title = "A synoviocyte model for osteoarthritis and rheumatoid arthritis: response to Ibuprofen, betamethasone, and ginger extract-a cross-sectional in vitro study",
abstract = "This study aimed at determining if synovial cell cultures from rheumatoid arthritis (RA), osteoarthritis (OA), and healthy controls (HC) differ and are suitable disease models in pharmacological studies, and tested their response to some anti-inflammatory drugs. Synovial cells were isolated from synovial membrane or joint fluid. Cells were cultivated and exposed to no or TNF-α stimulation without, or in the presence of, betamethasone, ibuprofen, or a standardized ginger extract. Concentrations of a panel of cytokines, growth factors, and chemokines were mapped for each culture and condition. Our cells secreted an increased amount of the cytokines IL-1β, IL-6, and IL-8 in response to TNF-α stimulation in all conditions. OA cells showed a higher IL-6 and IL-8 and a lower IL-1β production, when not stimulated, than RA and HC cells, which were similar. TNF-α stimulation caused similar IL-1β, IL-6, and IL-8 release in all groups. Ibuprofen showed no effect on cytokine production, while ginger extract was similar to betamethasone. Ginger extract was as effective an anti-inflammatory agent as betamethasone in this in vitro model. Cultured fibroblast-like synoviocytes from OA and RA subjects promise to be a useful pharmacological disease model, but further studies, to support results from such a model are needed.",
author = "S{\o}ren Ribel-Madsen and Bartels, {Else Marie} and Anders Stockmarr and Arne Borgwardt and Claus Cornett and Bente Danneskiold-Sams{\o}e and Henning Bliddal",
year = "2012",
doi = "10.1155/2012/505842",
language = "English",
volume = "2012",
pages = "505842",
journal = "Arthritis",
issn = "2090-1984",
publisher = "Hindawi Publishing Corporation",

}

RIS

TY - JOUR

T1 - A synoviocyte model for osteoarthritis and rheumatoid arthritis

T2 - response to Ibuprofen, betamethasone, and ginger extract-a cross-sectional in vitro study

AU - Ribel-Madsen, Søren

AU - Bartels, Else Marie

AU - Stockmarr, Anders

AU - Borgwardt, Arne

AU - Cornett, Claus

AU - Danneskiold-Samsøe, Bente

AU - Bliddal, Henning

PY - 2012

Y1 - 2012

N2 - This study aimed at determining if synovial cell cultures from rheumatoid arthritis (RA), osteoarthritis (OA), and healthy controls (HC) differ and are suitable disease models in pharmacological studies, and tested their response to some anti-inflammatory drugs. Synovial cells were isolated from synovial membrane or joint fluid. Cells were cultivated and exposed to no or TNF-α stimulation without, or in the presence of, betamethasone, ibuprofen, or a standardized ginger extract. Concentrations of a panel of cytokines, growth factors, and chemokines were mapped for each culture and condition. Our cells secreted an increased amount of the cytokines IL-1β, IL-6, and IL-8 in response to TNF-α stimulation in all conditions. OA cells showed a higher IL-6 and IL-8 and a lower IL-1β production, when not stimulated, than RA and HC cells, which were similar. TNF-α stimulation caused similar IL-1β, IL-6, and IL-8 release in all groups. Ibuprofen showed no effect on cytokine production, while ginger extract was similar to betamethasone. Ginger extract was as effective an anti-inflammatory agent as betamethasone in this in vitro model. Cultured fibroblast-like synoviocytes from OA and RA subjects promise to be a useful pharmacological disease model, but further studies, to support results from such a model are needed.

AB - This study aimed at determining if synovial cell cultures from rheumatoid arthritis (RA), osteoarthritis (OA), and healthy controls (HC) differ and are suitable disease models in pharmacological studies, and tested their response to some anti-inflammatory drugs. Synovial cells were isolated from synovial membrane or joint fluid. Cells were cultivated and exposed to no or TNF-α stimulation without, or in the presence of, betamethasone, ibuprofen, or a standardized ginger extract. Concentrations of a panel of cytokines, growth factors, and chemokines were mapped for each culture and condition. Our cells secreted an increased amount of the cytokines IL-1β, IL-6, and IL-8 in response to TNF-α stimulation in all conditions. OA cells showed a higher IL-6 and IL-8 and a lower IL-1β production, when not stimulated, than RA and HC cells, which were similar. TNF-α stimulation caused similar IL-1β, IL-6, and IL-8 release in all groups. Ibuprofen showed no effect on cytokine production, while ginger extract was similar to betamethasone. Ginger extract was as effective an anti-inflammatory agent as betamethasone in this in vitro model. Cultured fibroblast-like synoviocytes from OA and RA subjects promise to be a useful pharmacological disease model, but further studies, to support results from such a model are needed.

U2 - 10.1155/2012/505842

DO - 10.1155/2012/505842

M3 - Journal article

VL - 2012

SP - 505842

JO - Arthritis

JF - Arthritis

SN - 2090-1984

ER -

ID: 48430357