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A transgenic mouse line for molecular genetic analysis of excitatory glutamatergic neurons

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A transgenic mouse line for molecular genetic analysis of excitatory glutamatergic neurons. / Borgius, Lotta; Restrepo, C. Ernesto; Leao, Richardson N.; Saleh, Noor; Kiehn, Ole.

I: Molecular and Cellular Neuroscience, Bind 45, Nr. 3, 01.11.2010, s. 245-257.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Borgius, L, Restrepo, CE, Leao, RN, Saleh, N & Kiehn, O 2010, 'A transgenic mouse line for molecular genetic analysis of excitatory glutamatergic neurons', Molecular and Cellular Neuroscience, bind 45, nr. 3, s. 245-257. https://doi.org/10.1016/j.mcn.2010.06.016

APA

Borgius, L., Restrepo, C. E., Leao, R. N., Saleh, N., & Kiehn, O. (2010). A transgenic mouse line for molecular genetic analysis of excitatory glutamatergic neurons. Molecular and Cellular Neuroscience, 45(3), 245-257. https://doi.org/10.1016/j.mcn.2010.06.016

Vancouver

Borgius L, Restrepo CE, Leao RN, Saleh N, Kiehn O. A transgenic mouse line for molecular genetic analysis of excitatory glutamatergic neurons. Molecular and Cellular Neuroscience. 2010 nov 1;45(3):245-257. https://doi.org/10.1016/j.mcn.2010.06.016

Author

Borgius, Lotta ; Restrepo, C. Ernesto ; Leao, Richardson N. ; Saleh, Noor ; Kiehn, Ole. / A transgenic mouse line for molecular genetic analysis of excitatory glutamatergic neurons. I: Molecular and Cellular Neuroscience. 2010 ; Bind 45, Nr. 3. s. 245-257.

Bibtex

@article{435f9cfd37ce43c2a82b197cf77be071,
title = "A transgenic mouse line for molecular genetic analysis of excitatory glutamatergic neurons",
abstract = "Excitatory glutamatergic neurons are part of most of the neuronal circuits in the mammalian nervous system. We have used BAC-technology to generate a BAC-Vglut2::Cre mouse line where Cre expression is driven by the vesicular glutamate transporter 2 (Vglut2) promotor. This BAC-Vglut2::Cre mouse line showed specific expression of Cre in Vglut2 positive cells in the spinal cord with no ectopic expression in GABAergic or glycinergic neurons. This mouse line also showed specific Cre expression in Vglut2 positive structures in the brain such as thalamus, hypothalamus, superior colliculi, inferior colliculi and deep cerebellar nuclei together with nuclei in the midbrain and hindbrain. Cre-mediated recombination was restricted to Cre expressing cells in the spinal cord and brain and occurred as early as E 12.5. Known Vglut2 positive neurons showed normal electrophysiological properties in the BAC-Vglut2::Cre transgenic mice. Altogether, this BAC-Vglut2::Cre mouse line provides a valuable tool for molecular genetic analysis of excitatory neuronal populations throughout the mouse nervous system.",
keywords = "Cre-recombinase, Glutamate, Interneuron, Transgenic mouse, Vesicular glutamate transporters",
author = "Lotta Borgius and Restrepo, {C. Ernesto} and Leao, {Richardson N.} and Noor Saleh and Ole Kiehn",
year = "2010",
month = nov,
day = "1",
doi = "10.1016/j.mcn.2010.06.016",
language = "English",
volume = "45",
pages = "245--257",
journal = "Molecular and Cellular Neuroscience",
issn = "1044-7431",
publisher = "Academic Press",
number = "3",

}

RIS

TY - JOUR

T1 - A transgenic mouse line for molecular genetic analysis of excitatory glutamatergic neurons

AU - Borgius, Lotta

AU - Restrepo, C. Ernesto

AU - Leao, Richardson N.

AU - Saleh, Noor

AU - Kiehn, Ole

PY - 2010/11/1

Y1 - 2010/11/1

N2 - Excitatory glutamatergic neurons are part of most of the neuronal circuits in the mammalian nervous system. We have used BAC-technology to generate a BAC-Vglut2::Cre mouse line where Cre expression is driven by the vesicular glutamate transporter 2 (Vglut2) promotor. This BAC-Vglut2::Cre mouse line showed specific expression of Cre in Vglut2 positive cells in the spinal cord with no ectopic expression in GABAergic or glycinergic neurons. This mouse line also showed specific Cre expression in Vglut2 positive structures in the brain such as thalamus, hypothalamus, superior colliculi, inferior colliculi and deep cerebellar nuclei together with nuclei in the midbrain and hindbrain. Cre-mediated recombination was restricted to Cre expressing cells in the spinal cord and brain and occurred as early as E 12.5. Known Vglut2 positive neurons showed normal electrophysiological properties in the BAC-Vglut2::Cre transgenic mice. Altogether, this BAC-Vglut2::Cre mouse line provides a valuable tool for molecular genetic analysis of excitatory neuronal populations throughout the mouse nervous system.

AB - Excitatory glutamatergic neurons are part of most of the neuronal circuits in the mammalian nervous system. We have used BAC-technology to generate a BAC-Vglut2::Cre mouse line where Cre expression is driven by the vesicular glutamate transporter 2 (Vglut2) promotor. This BAC-Vglut2::Cre mouse line showed specific expression of Cre in Vglut2 positive cells in the spinal cord with no ectopic expression in GABAergic or glycinergic neurons. This mouse line also showed specific Cre expression in Vglut2 positive structures in the brain such as thalamus, hypothalamus, superior colliculi, inferior colliculi and deep cerebellar nuclei together with nuclei in the midbrain and hindbrain. Cre-mediated recombination was restricted to Cre expressing cells in the spinal cord and brain and occurred as early as E 12.5. Known Vglut2 positive neurons showed normal electrophysiological properties in the BAC-Vglut2::Cre transgenic mice. Altogether, this BAC-Vglut2::Cre mouse line provides a valuable tool for molecular genetic analysis of excitatory neuronal populations throughout the mouse nervous system.

KW - Cre-recombinase

KW - Glutamate

KW - Interneuron

KW - Transgenic mouse

KW - Vesicular glutamate transporters

UR - http://www.scopus.com/inward/record.url?scp=77956611694&partnerID=8YFLogxK

U2 - 10.1016/j.mcn.2010.06.016

DO - 10.1016/j.mcn.2010.06.016

M3 - Journal article

C2 - 20600924

AN - SCOPUS:77956611694

VL - 45

SP - 245

EP - 257

JO - Molecular and Cellular Neuroscience

JF - Molecular and Cellular Neuroscience

SN - 1044-7431

IS - 3

ER -

ID: 194977830