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A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Dokumenter

  • Yukie M. Lloyd
  • Elise P. Ngati
  • Salanti, Ali
  • Rose G.F. Leke
  • Diane W. Taylor

Antibody-mediated phagocytosis is an important immune effector mechanism against Plasmodium falciparum-infected erythrocytes (IE); however, current phagocytosis assays use IE collected from infected individuals or from in vitro cultures of P. falciparum, making them prone to high variation. A simple, high-throughput flow cytometric assay was developed that uses THP-1 cells and fluorescent beads covalently-coupled with the malarial antigen VAR2CSA. The assay is highly repeatable, provides both the overall percent phagocytosis and semi-quantitates the number of antigen-coupled beads internalized.

OriginalsprogEngelsk
Artikelnummer14705
TidsskriftScientific Reports
Vol/bind7
Antal sider7
ISSN2045-2322
DOI
StatusUdgivet - 2017

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