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Accumulation of zinc in human atherosclerotic lesions correlates with calcium levels but does not protect against protein oxidation

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Nadina Stadler
  • Naomi Stanley
  • Sylvia Heeneman
  • Vladimir Vacata
  • Mat J A P Daemen
  • Paul G Bannon
  • Johannes Waltenberger
  • Davies, Michael Jonathan

OBJECTIVE: Oxidized lipids and proteins, as well as decreased antioxidant levels, have been detected in human atherosclerotic lesions, with oxidation catalyzed by iron and copper postulated to contribute to lesion development. Zinc has been postulated to displace iron from critical sites and thereby protect against damage. In this study, metal ion and protein oxidation levels were quantified in human carotid and abdominal artery specimens containing early-to-advanced lesions, to determine whether zinc concentrations correlate inversely with iron levels and protein oxidation.

METHODS AND RESULTS: Metal ions were quantified by EPR and inductively coupled plasma mass spectroscopy. Native and oxidized protein side-chains were quantified by high-performance liquid chromatography. Elevated levels of zinc ( approximately 6-fold) were detected in advanced lesions compared to healthy tissue or early lesions. Zinc did not correlate negatively with iron or copper levels suggesting that zinc does not displace these metal ions. Highly significant positive correlations (P<0.005) were detected between zinc and calcium levels.

CONCLUSIONS: Zinc did not correlate with low iron levels and reduced protein oxidation. These data indicate that zinc does not prevent protein oxidation in advanced lesions. The reported protective effect of zinc accumulation is proposed to be associated with lesion calcification.

OriginalsprogEngelsk
TidsskriftArteriosclerosis, Thrombosis, and Vascular Biology
Vol/bind28
Udgave nummer5
Sider (fra-til)1024-30
Antal sider7
ISSN1079-5642
DOI
StatusUdgivet - maj 2008
Eksternt udgivetJa

ID: 129670859