Forskning ved Københavns Universitet - Københavns Universitet

Forside

Adaptive evolution of cytochrome c oxidase: Infrastructure for a carnivorous plant radiation

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Adaptive evolution of cytochrome c oxidase : Infrastructure for a carnivorous plant radiation. / Jobson, Richard W.; Nielsen, Rasmus; Laakkonen, Liisa; Wikström, Mårten; Albert, Victor A.

I: Proceedings of the National Academy of Sciences of the United States of America, Bind 101, Nr. 52, 2004, s. 18064-18068.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Jobson, RW, Nielsen, R, Laakkonen, L, Wikström, M & Albert, VA 2004, 'Adaptive evolution of cytochrome c oxidase: Infrastructure for a carnivorous plant radiation', Proceedings of the National Academy of Sciences of the United States of America, bind 101, nr. 52, s. 18064-18068. https://doi.org/10.1073/pnas.0408092101

APA

Jobson, R. W., Nielsen, R., Laakkonen, L., Wikström, M., & Albert, V. A. (2004). Adaptive evolution of cytochrome c oxidase: Infrastructure for a carnivorous plant radiation. Proceedings of the National Academy of Sciences of the United States of America, 101(52), 18064-18068. https://doi.org/10.1073/pnas.0408092101

Vancouver

Jobson RW, Nielsen R, Laakkonen L, Wikström M, Albert VA. Adaptive evolution of cytochrome c oxidase: Infrastructure for a carnivorous plant radiation. Proceedings of the National Academy of Sciences of the United States of America. 2004;101(52):18064-18068. https://doi.org/10.1073/pnas.0408092101

Author

Jobson, Richard W. ; Nielsen, Rasmus ; Laakkonen, Liisa ; Wikström, Mårten ; Albert, Victor A. / Adaptive evolution of cytochrome c oxidase : Infrastructure for a carnivorous plant radiation. I: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Bind 101, Nr. 52. s. 18064-18068.

Bibtex

@article{617d9a0b5fe74162b12a92d5903234ef,
title = "Adaptive evolution of cytochrome c oxidase: Infrastructure for a carnivorous plant radiation",
abstract = "Much recent attention in the study of adaptation of organismal form has centered on developmental regulation. As such, the highly conserved respiratory machinery of eukaryotic cells might seem an unlikely target for selection supporting novel morphologies. We demonstrate that a dramatic molecular evolutionary rate increase in subunit I of cytochrome c oxidase (COX) from an active-trapping lineage of carnivorous plants is caused by positive Darwinian selection. Bladderworts (Utricularia) trap plankton when water-immersed, negatively pressured suction bladders are triggered. The resetting of traps involves active ion transport, requiring considerable energy expenditure. As judged from the quaternary structure of bovine COX, the most profound adaptive substitutions are two contiguous cysteines absent in ≈99.9{\%} of databased COX I sequences from Eukaryota, Archaea, and Bacteria. This motif lies directly at the docking point of COX I helix 3 and cytochrome c, and modeling of bovine COX I suggests the possibility of an unprecedented helix-terminating disulfide bridge that could alter COX, cytochrome c dissociation kinetics. Thus, the key adaptation in Utricularia likely lies in molecular energetic changes that buttressed the mechanisms responsible for the bladderworts' radical morphological evolution. Along with evidence for COX evolution underlying expansion of the anthropoid neocortex, our findings underscore that important morphological and physiological innovations must often be accompanied by specific adaptations in proteins with basic cellular functions.",
keywords = "Cellular energetics, Developmental regulation, Molecular adaptation, Positive selection, Protein structure",
author = "Jobson, {Richard W.} and Rasmus Nielsen and Liisa Laakkonen and M{\aa}rten Wikstr{\"o}m and Albert, {Victor A.}",
year = "2004",
doi = "10.1073/pnas.0408092101",
language = "English",
volume = "101",
pages = "18064--18068",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "52",

}

RIS

TY - JOUR

T1 - Adaptive evolution of cytochrome c oxidase

T2 - Infrastructure for a carnivorous plant radiation

AU - Jobson, Richard W.

AU - Nielsen, Rasmus

AU - Laakkonen, Liisa

AU - Wikström, Mårten

AU - Albert, Victor A.

PY - 2004

Y1 - 2004

N2 - Much recent attention in the study of adaptation of organismal form has centered on developmental regulation. As such, the highly conserved respiratory machinery of eukaryotic cells might seem an unlikely target for selection supporting novel morphologies. We demonstrate that a dramatic molecular evolutionary rate increase in subunit I of cytochrome c oxidase (COX) from an active-trapping lineage of carnivorous plants is caused by positive Darwinian selection. Bladderworts (Utricularia) trap plankton when water-immersed, negatively pressured suction bladders are triggered. The resetting of traps involves active ion transport, requiring considerable energy expenditure. As judged from the quaternary structure of bovine COX, the most profound adaptive substitutions are two contiguous cysteines absent in ≈99.9% of databased COX I sequences from Eukaryota, Archaea, and Bacteria. This motif lies directly at the docking point of COX I helix 3 and cytochrome c, and modeling of bovine COX I suggests the possibility of an unprecedented helix-terminating disulfide bridge that could alter COX, cytochrome c dissociation kinetics. Thus, the key adaptation in Utricularia likely lies in molecular energetic changes that buttressed the mechanisms responsible for the bladderworts' radical morphological evolution. Along with evidence for COX evolution underlying expansion of the anthropoid neocortex, our findings underscore that important morphological and physiological innovations must often be accompanied by specific adaptations in proteins with basic cellular functions.

AB - Much recent attention in the study of adaptation of organismal form has centered on developmental regulation. As such, the highly conserved respiratory machinery of eukaryotic cells might seem an unlikely target for selection supporting novel morphologies. We demonstrate that a dramatic molecular evolutionary rate increase in subunit I of cytochrome c oxidase (COX) from an active-trapping lineage of carnivorous plants is caused by positive Darwinian selection. Bladderworts (Utricularia) trap plankton when water-immersed, negatively pressured suction bladders are triggered. The resetting of traps involves active ion transport, requiring considerable energy expenditure. As judged from the quaternary structure of bovine COX, the most profound adaptive substitutions are two contiguous cysteines absent in ≈99.9% of databased COX I sequences from Eukaryota, Archaea, and Bacteria. This motif lies directly at the docking point of COX I helix 3 and cytochrome c, and modeling of bovine COX I suggests the possibility of an unprecedented helix-terminating disulfide bridge that could alter COX, cytochrome c dissociation kinetics. Thus, the key adaptation in Utricularia likely lies in molecular energetic changes that buttressed the mechanisms responsible for the bladderworts' radical morphological evolution. Along with evidence for COX evolution underlying expansion of the anthropoid neocortex, our findings underscore that important morphological and physiological innovations must often be accompanied by specific adaptations in proteins with basic cellular functions.

KW - Cellular energetics

KW - Developmental regulation

KW - Molecular adaptation

KW - Positive selection

KW - Protein structure

U2 - 10.1073/pnas.0408092101

DO - 10.1073/pnas.0408092101

M3 - Journal article

C2 - 15596720

AN - SCOPUS:11144246408

VL - 101

SP - 18064

EP - 18068

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 52

ER -

ID: 222644608