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Adipose triglyceride lipase plays a key role in the supply of the working muscle with fatty acids

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Gabriele Schoiswohl
  • Martina Schweiger
  • Renate Schreiber
  • Gregor Gorkiewicz
  • Karina Preiss-Landl
  • Ulrike Taschler
  • Kathrin A. Zierler
  • Franz P. W. Radner
  • Thomas O. Eichmann
  • Petra C Kienesberger
  • Sandra Eder
  • Achim Lass
  • Guenter Haemmerle
  • Thomas Junker Alsted
  • Kiens, Bente
  • Gerald Hoefler
  • Rudolf Zechner
  • Robert Zimmermann
Fatty acids (FA) are mobilized from triglyceride (TG) stores during exercise to supply the working muscle with energy. Mice deficient for adipose triglyceride lipase (ATGLko)exhibit defective lipolysis and accumulate TG in adipose tissue and muscle suggesting that ATGL deficiency affects energy availability and substrate utilization in working muscle. In this study, we investigated the effect of moderate treadmill exercise on blood energy metabolites and liver glycogen stores in mice lacking ATGL. Since ATGL-ko mice exhibit massive accumulation of TG in the heart and cardiomyopathy, we also investigated a mouse model lacking ATGL in all tissues except cardiac muscle (ATGL-ko/CM). In contrast to ATGL-ko mice, these mice did not accumulate TG in the heart and had normal life expectancy. Exercise experiments revealed that ATGL-ko and ATGL-ko/CM mice are unable to increase circulating FA levels during exercise. The reduced availability of FA for energy conversion led to rapid depletion of liver glycogen stores and hypoglycemia. Together, our studies suggest that ATGL-ko mice cannot adjust circulating FA levels to the increased energy requirements of the working muscle resulting in an increased use of carbohydrates for energy conversion. Thus, ATGL activity is required for proper energy supply of the skeletal muscle during exercise.
OriginalsprogEngelsk
TidsskriftJournal of Lipid Research
Vol/bind51
Udgave nummer3
Sider (fra-til)490-499
Antal sider10
ISSN0022-2275
DOI
StatusUdgivet - 2010

Bibliografisk note

CURIS 2010 5200 012

ID: 17111889