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Advances and perspectives in tissue clearing using CLARITY

Publikation: Bidrag til tidsskriftReviewForskningfagfællebedømt

CLARITY is a tissue clearing method, which enables immunostaining and imaging of large volumes for 3D-reconstruction. The method was initially time-consuming, expensive and relied on electrophoresis to remove lipids to make the tissue transparent. Since then several improvements and simplifications have emerged, such as passive clearing (PACT) and methods to improve tissue staining. Here, we review advances and compare current applications with the aim of highlighting needed improvements as well as aiding selection of the specific protocol for use in future investigations.
OriginalsprogEngelsk
TidsskriftJournal of Chemical Neuroanatomy
Vol/bind86
Sider (fra-til)19-34
Antal sider16
ISSN0891-0618
DOI
StatusUdgivet - dec. 2017

ID: 181230476