Forskning ved Københavns Universitet - Københavns Universitet

Forside

Altered expression of epithelial cell surface glycoconjugates and intermediate filaments at the margins of mucosal wounds

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Altered expression of epithelial cell surface glycoconjugates and intermediate filaments at the margins of mucosal wounds. / Dabelsteen, Erik; Grøn, B; Mandel, U; Mackenzie, I.

I: Journal of Investigative Dermatology, Bind 111, Nr. 4, 1998, s. 592-7.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Dabelsteen, E, Grøn, B, Mandel, U & Mackenzie, I 1998, 'Altered expression of epithelial cell surface glycoconjugates and intermediate filaments at the margins of mucosal wounds', Journal of Investigative Dermatology, bind 111, nr. 4, s. 592-7. https://doi.org/10.1046/j.1523-1747.1998.00346.x

APA

Dabelsteen, E., Grøn, B., Mandel, U., & Mackenzie, I. (1998). Altered expression of epithelial cell surface glycoconjugates and intermediate filaments at the margins of mucosal wounds. Journal of Investigative Dermatology, 111(4), 592-7. https://doi.org/10.1046/j.1523-1747.1998.00346.x

Vancouver

Dabelsteen E, Grøn B, Mandel U, Mackenzie I. Altered expression of epithelial cell surface glycoconjugates and intermediate filaments at the margins of mucosal wounds. Journal of Investigative Dermatology. 1998;111(4):592-7. https://doi.org/10.1046/j.1523-1747.1998.00346.x

Author

Dabelsteen, Erik ; Grøn, B ; Mandel, U ; Mackenzie, I. / Altered expression of epithelial cell surface glycoconjugates and intermediate filaments at the margins of mucosal wounds. I: Journal of Investigative Dermatology. 1998 ; Bind 111, Nr. 4. s. 592-7.

Bibtex

@article{c0b773232bb54aeba25a50b9400df2dd,
title = "Altered expression of epithelial cell surface glycoconjugates and intermediate filaments at the margins of mucosal wounds",
abstract = "Alterations in cell to cell adhesion are necessary to enable the type of cell movements that are associated with epithelial wound healing and malignant invasion. Several studies of transformed cells have related epithelial cell movement to changes in the cell surface expression of the carbohydrate structures represented by the ABO blood group antigens and, in particular, by Lewis antigens and their biosynthetic precursors. To study further the relationship between cell surface carbohydrates and keratinocyte cell movement, experimental wounds were created in human oral mucosa and examined by immunohistochemical methods for their expression of selected cytokeratins (K5, K16, K19), basement membrane components (laminin alpha5 and gamma2-chains, BP180, collagen IV and collagen VII), and blood group antigen precursor structures Le(x), sialosyl-Le(x), Le(y), H antigen, N-acetyllactosamine, and sialosyl-T antigen. The changes induced by wounding in the expression of collagen IV, laminin gamma2-chain (laminin-5), and laminin alpha5-chain were similar to those found in skin wounds and served to define the region of epithelial movement. This region was found to show a marked increase in staining for both Lewis antigen Y (Le(y)) and H blood group antigen, and decreased staining of Le(x), thus indicating an upregulation in wounded epithelium of the fucosyltransferases responsible for the synthesis of the H antigen. The changes in carbohydrate expression extended beyond the wound margin into the nonwounded epithelium, a pattern of expression similar to K16, which was also strongly upregulated in both the outgrowth and the adjacent nonwounded epithelium. These findings provide further support for an influence of such carbohydrate structures on the migratory behavior of epithelial cells.",
keywords = "Adult, Antibodies, Cell Movement, Glycoconjugates, Humans, Keratins, Laminin, Lewis Blood-Group System, Membrane Glycoproteins, Mouth Mucosa, Tissue Distribution, Wound Healing, Wounds and Injuries",
author = "Erik Dabelsteen and B Gr{\o}n and U Mandel and I Mackenzie",
year = "1998",
doi = "10.1046/j.1523-1747.1998.00346.x",
language = "English",
volume = "111",
pages = "592--7",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "nature publishing group",
number = "4",

}

RIS

TY - JOUR

T1 - Altered expression of epithelial cell surface glycoconjugates and intermediate filaments at the margins of mucosal wounds

AU - Dabelsteen, Erik

AU - Grøn, B

AU - Mandel, U

AU - Mackenzie, I

PY - 1998

Y1 - 1998

N2 - Alterations in cell to cell adhesion are necessary to enable the type of cell movements that are associated with epithelial wound healing and malignant invasion. Several studies of transformed cells have related epithelial cell movement to changes in the cell surface expression of the carbohydrate structures represented by the ABO blood group antigens and, in particular, by Lewis antigens and their biosynthetic precursors. To study further the relationship between cell surface carbohydrates and keratinocyte cell movement, experimental wounds were created in human oral mucosa and examined by immunohistochemical methods for their expression of selected cytokeratins (K5, K16, K19), basement membrane components (laminin alpha5 and gamma2-chains, BP180, collagen IV and collagen VII), and blood group antigen precursor structures Le(x), sialosyl-Le(x), Le(y), H antigen, N-acetyllactosamine, and sialosyl-T antigen. The changes induced by wounding in the expression of collagen IV, laminin gamma2-chain (laminin-5), and laminin alpha5-chain were similar to those found in skin wounds and served to define the region of epithelial movement. This region was found to show a marked increase in staining for both Lewis antigen Y (Le(y)) and H blood group antigen, and decreased staining of Le(x), thus indicating an upregulation in wounded epithelium of the fucosyltransferases responsible for the synthesis of the H antigen. The changes in carbohydrate expression extended beyond the wound margin into the nonwounded epithelium, a pattern of expression similar to K16, which was also strongly upregulated in both the outgrowth and the adjacent nonwounded epithelium. These findings provide further support for an influence of such carbohydrate structures on the migratory behavior of epithelial cells.

AB - Alterations in cell to cell adhesion are necessary to enable the type of cell movements that are associated with epithelial wound healing and malignant invasion. Several studies of transformed cells have related epithelial cell movement to changes in the cell surface expression of the carbohydrate structures represented by the ABO blood group antigens and, in particular, by Lewis antigens and their biosynthetic precursors. To study further the relationship between cell surface carbohydrates and keratinocyte cell movement, experimental wounds were created in human oral mucosa and examined by immunohistochemical methods for their expression of selected cytokeratins (K5, K16, K19), basement membrane components (laminin alpha5 and gamma2-chains, BP180, collagen IV and collagen VII), and blood group antigen precursor structures Le(x), sialosyl-Le(x), Le(y), H antigen, N-acetyllactosamine, and sialosyl-T antigen. The changes induced by wounding in the expression of collagen IV, laminin gamma2-chain (laminin-5), and laminin alpha5-chain were similar to those found in skin wounds and served to define the region of epithelial movement. This region was found to show a marked increase in staining for both Lewis antigen Y (Le(y)) and H blood group antigen, and decreased staining of Le(x), thus indicating an upregulation in wounded epithelium of the fucosyltransferases responsible for the synthesis of the H antigen. The changes in carbohydrate expression extended beyond the wound margin into the nonwounded epithelium, a pattern of expression similar to K16, which was also strongly upregulated in both the outgrowth and the adjacent nonwounded epithelium. These findings provide further support for an influence of such carbohydrate structures on the migratory behavior of epithelial cells.

KW - Adult

KW - Antibodies

KW - Cell Movement

KW - Glycoconjugates

KW - Humans

KW - Keratins

KW - Laminin

KW - Lewis Blood-Group System

KW - Membrane Glycoproteins

KW - Mouth Mucosa

KW - Tissue Distribution

KW - Wound Healing

KW - Wounds and Injuries

U2 - 10.1046/j.1523-1747.1998.00346.x

DO - 10.1046/j.1523-1747.1998.00346.x

M3 - Journal article

C2 - 9764838

VL - 111

SP - 592

EP - 597

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 4

ER -

ID: 119593850