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Amino acid δ13C analysis of hair proteins and bone collagen using liquid chromatography/isotope ratio mass spectrometry: paleodietary implications from intra-individual comparisons

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Standard

Amino acid δ13C analysis of hair proteins and bone collagen using liquid chromatography/isotope ratio mass spectrometry : paleodietary implications from intra-individual comparisons. / Raghavan, Maanasa; McCullagh, James S. O.; Lynnerup, Niels; Hedges, Robert E. M.

I: Rapid Communications in Mass Spectrometry, Bind 24, Nr. 5, 2010, s. 541-548.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Raghavan, M, McCullagh, JSO, Lynnerup, N & Hedges, REM 2010, 'Amino acid δ13C analysis of hair proteins and bone collagen using liquid chromatography/isotope ratio mass spectrometry: paleodietary implications from intra-individual comparisons', Rapid Communications in Mass Spectrometry, bind 24, nr. 5, s. 541-548. https://doi.org/10.1002/rcm.4398

APA

Raghavan, M., McCullagh, J. S. O., Lynnerup, N., & Hedges, R. E. M. (2010). Amino acid δ13C analysis of hair proteins and bone collagen using liquid chromatography/isotope ratio mass spectrometry: paleodietary implications from intra-individual comparisons. Rapid Communications in Mass Spectrometry, 24(5), 541-548. https://doi.org/10.1002/rcm.4398

Vancouver

Raghavan M, McCullagh JSO, Lynnerup N, Hedges REM. Amino acid δ13C analysis of hair proteins and bone collagen using liquid chromatography/isotope ratio mass spectrometry: paleodietary implications from intra-individual comparisons. Rapid Communications in Mass Spectrometry. 2010;24(5):541-548. https://doi.org/10.1002/rcm.4398

Author

Raghavan, Maanasa ; McCullagh, James S. O. ; Lynnerup, Niels ; Hedges, Robert E. M. / Amino acid δ13C analysis of hair proteins and bone collagen using liquid chromatography/isotope ratio mass spectrometry : paleodietary implications from intra-individual comparisons. I: Rapid Communications in Mass Spectrometry. 2010 ; Bind 24, Nr. 5. s. 541-548.

Bibtex

@article{ae8196d09e4311df928f000ea68e967b,
title = "Amino acid δ13C analysis of hair proteins and bone collagen using liquid chromatography/isotope ratio mass spectrometry: paleodietary implications from intra-individual comparisons",
abstract = "We report a novel method for the chromatographic separation and measurement of stable carbon isotope ratios (delta(13)C) of individual amino acids in hair proteins and bone collagen using the LC-IsoLink system, which interfaces liquid chromatography (LC) with isotope ratio mass spectrometry (IRMS). This paper provides baseline separation of 15 and 13 of the 18 amino acids in bone collagen and hair proteins, respectively. We also describe an approach to analysing small hair samples for compound-specific analysis of segmental hair sections. The LC/IRMS method is applied in a historical context by the delta(13)C analysis of hair proteins and bone collagen recovered from six individuals from Uummannaq in Greenland. The analysis of hair and bone amino acids from the same individual, compared for the first time in this study, is of importance in palaeodietary reconstruction. If hair proteins can be used as a proxy for bone collagen at the amino acid level, this validates compound-specific isotope studies using hair as a model for palaeodietary reconstruction. Our results suggest that a small offset observed in the bulk delta(13)C values of the hair and bone samples may be attributed to two factors: (i) amino acid compositional differences between hair and bone proteins, and (ii) differential turnover rates of the tissues and the amino acid pools contributing to their synthesis. This application proposes that hair may be a useful complementary or alternative source of compound-specific paleodietary information.",
author = "Maanasa Raghavan and McCullagh, {James S. O.} and Niels Lynnerup and Hedges, {Robert E. M.}",
note = "Keywords: Amino Acids; Bone and Bones; Carbon Isotopes; Chromatography, Liquid; Collagen; Diet; Humans; Keratins, Hair-Specific; Mass Spectrometry; Mummies",
year = "2010",
doi = "10.1002/rcm.4398",
language = "English",
volume = "24",
pages = "541--548",
journal = "Rapid Communications in Mass Spectrometry",
issn = "0951-4198",
publisher = "JohnWiley & Sons Ltd",
number = "5",

}

RIS

TY - JOUR

T1 - Amino acid δ13C analysis of hair proteins and bone collagen using liquid chromatography/isotope ratio mass spectrometry

T2 - paleodietary implications from intra-individual comparisons

AU - Raghavan, Maanasa

AU - McCullagh, James S. O.

AU - Lynnerup, Niels

AU - Hedges, Robert E. M.

N1 - Keywords: Amino Acids; Bone and Bones; Carbon Isotopes; Chromatography, Liquid; Collagen; Diet; Humans; Keratins, Hair-Specific; Mass Spectrometry; Mummies

PY - 2010

Y1 - 2010

N2 - We report a novel method for the chromatographic separation and measurement of stable carbon isotope ratios (delta(13)C) of individual amino acids in hair proteins and bone collagen using the LC-IsoLink system, which interfaces liquid chromatography (LC) with isotope ratio mass spectrometry (IRMS). This paper provides baseline separation of 15 and 13 of the 18 amino acids in bone collagen and hair proteins, respectively. We also describe an approach to analysing small hair samples for compound-specific analysis of segmental hair sections. The LC/IRMS method is applied in a historical context by the delta(13)C analysis of hair proteins and bone collagen recovered from six individuals from Uummannaq in Greenland. The analysis of hair and bone amino acids from the same individual, compared for the first time in this study, is of importance in palaeodietary reconstruction. If hair proteins can be used as a proxy for bone collagen at the amino acid level, this validates compound-specific isotope studies using hair as a model for palaeodietary reconstruction. Our results suggest that a small offset observed in the bulk delta(13)C values of the hair and bone samples may be attributed to two factors: (i) amino acid compositional differences between hair and bone proteins, and (ii) differential turnover rates of the tissues and the amino acid pools contributing to their synthesis. This application proposes that hair may be a useful complementary or alternative source of compound-specific paleodietary information.

AB - We report a novel method for the chromatographic separation and measurement of stable carbon isotope ratios (delta(13)C) of individual amino acids in hair proteins and bone collagen using the LC-IsoLink system, which interfaces liquid chromatography (LC) with isotope ratio mass spectrometry (IRMS). This paper provides baseline separation of 15 and 13 of the 18 amino acids in bone collagen and hair proteins, respectively. We also describe an approach to analysing small hair samples for compound-specific analysis of segmental hair sections. The LC/IRMS method is applied in a historical context by the delta(13)C analysis of hair proteins and bone collagen recovered from six individuals from Uummannaq in Greenland. The analysis of hair and bone amino acids from the same individual, compared for the first time in this study, is of importance in palaeodietary reconstruction. If hair proteins can be used as a proxy for bone collagen at the amino acid level, this validates compound-specific isotope studies using hair as a model for palaeodietary reconstruction. Our results suggest that a small offset observed in the bulk delta(13)C values of the hair and bone samples may be attributed to two factors: (i) amino acid compositional differences between hair and bone proteins, and (ii) differential turnover rates of the tissues and the amino acid pools contributing to their synthesis. This application proposes that hair may be a useful complementary or alternative source of compound-specific paleodietary information.

U2 - 10.1002/rcm.4398

DO - 10.1002/rcm.4398

M3 - Journal article

C2 - 20131322

VL - 24

SP - 541

EP - 548

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

SN - 0951-4198

IS - 5

ER -

ID: 21138998