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Aminopeptidase N is directly sorted to the apical domain in MDCK cells

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Standard

Aminopeptidase N is directly sorted to the apical domain in MDCK cells. / Wessels, H P; Hansen, Gert Helge; Fuhrer, C; Look, A T; Sjöström, H; Norén, Ove; Spiess, M.

I: Journal of Cell Biology, Bind 111, Nr. 6 Pt 2, 1990, s. 2923-30.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Wessels, HP, Hansen, GH, Fuhrer, C, Look, AT, Sjöström, H, Norén, O & Spiess, M 1990, 'Aminopeptidase N is directly sorted to the apical domain in MDCK cells', Journal of Cell Biology, bind 111, nr. 6 Pt 2, s. 2923-30.

APA

Wessels, H. P., Hansen, G. H., Fuhrer, C., Look, A. T., Sjöström, H., Norén, O., & Spiess, M. (1990). Aminopeptidase N is directly sorted to the apical domain in MDCK cells. Journal of Cell Biology, 111(6 Pt 2), 2923-30.

Vancouver

Wessels HP, Hansen GH, Fuhrer C, Look AT, Sjöström H, Norén O o.a. Aminopeptidase N is directly sorted to the apical domain in MDCK cells. Journal of Cell Biology. 1990;111(6 Pt 2):2923-30.

Author

Wessels, H P ; Hansen, Gert Helge ; Fuhrer, C ; Look, A T ; Sjöström, H ; Norén, Ove ; Spiess, M. / Aminopeptidase N is directly sorted to the apical domain in MDCK cells. I: Journal of Cell Biology. 1990 ; Bind 111, Nr. 6 Pt 2. s. 2923-30.

Bibtex

@article{c5b83de0e31411ddb5fc000ea68e967b,
title = "Aminopeptidase N is directly sorted to the apical domain in MDCK cells",
abstract = "In different epithelial cell types, integral membrane proteins appear to follow different sorting pathways to the apical surface. In hepatocytes, several apical proteins were shown to be transported there indirectly via the basolateral membrane, whereas in MDCK cells a direct sorting pathway from the trans-Golgi-network to the apical membrane has been demonstrated. However, different proteins had been studied in these cells. To compare the sorting of a single protein in both systems, we have expressed aminopeptidase N, which already had been shown to be sorted indirectly in hepatocytes, in transfected MDCK cells. As expected, it was predominantly localized to the apical domain of the plasma membrane. By monitoring the appearance of newly synthesized aminopeptidase N at the apical and basolateral surface, it was found to be directly sorted to the apical domain in MDCK cells, indicating that the sorting pathways are indeed cell type-specific.",
author = "Wessels, {H P} and Hansen, {Gert Helge} and C Fuhrer and Look, {A T} and H Sj{\"o}str{\"o}m and Ove Nor{\'e}n and M Spiess",
note = "Keywords: Aminopeptidases; Animals; Antigens, CD13; Biological Transport; Cells, Cultured; Epithelium; Humans; Membrane Proteins; Recombinant Proteins; Transfection",
year = "1990",
language = "English",
volume = "111",
pages = "2923--30",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "6 Pt 2",

}

RIS

TY - JOUR

T1 - Aminopeptidase N is directly sorted to the apical domain in MDCK cells

AU - Wessels, H P

AU - Hansen, Gert Helge

AU - Fuhrer, C

AU - Look, A T

AU - Sjöström, H

AU - Norén, Ove

AU - Spiess, M

N1 - Keywords: Aminopeptidases; Animals; Antigens, CD13; Biological Transport; Cells, Cultured; Epithelium; Humans; Membrane Proteins; Recombinant Proteins; Transfection

PY - 1990

Y1 - 1990

N2 - In different epithelial cell types, integral membrane proteins appear to follow different sorting pathways to the apical surface. In hepatocytes, several apical proteins were shown to be transported there indirectly via the basolateral membrane, whereas in MDCK cells a direct sorting pathway from the trans-Golgi-network to the apical membrane has been demonstrated. However, different proteins had been studied in these cells. To compare the sorting of a single protein in both systems, we have expressed aminopeptidase N, which already had been shown to be sorted indirectly in hepatocytes, in transfected MDCK cells. As expected, it was predominantly localized to the apical domain of the plasma membrane. By monitoring the appearance of newly synthesized aminopeptidase N at the apical and basolateral surface, it was found to be directly sorted to the apical domain in MDCK cells, indicating that the sorting pathways are indeed cell type-specific.

AB - In different epithelial cell types, integral membrane proteins appear to follow different sorting pathways to the apical surface. In hepatocytes, several apical proteins were shown to be transported there indirectly via the basolateral membrane, whereas in MDCK cells a direct sorting pathway from the trans-Golgi-network to the apical membrane has been demonstrated. However, different proteins had been studied in these cells. To compare the sorting of a single protein in both systems, we have expressed aminopeptidase N, which already had been shown to be sorted indirectly in hepatocytes, in transfected MDCK cells. As expected, it was predominantly localized to the apical domain of the plasma membrane. By monitoring the appearance of newly synthesized aminopeptidase N at the apical and basolateral surface, it was found to be directly sorted to the apical domain in MDCK cells, indicating that the sorting pathways are indeed cell type-specific.

M3 - Journal article

C2 - 1980123

VL - 111

SP - 2923

EP - 2930

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 6 Pt 2

ER -

ID: 9748730