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Forside

An alternatively spliced fibroblast growth factor (FGF)-5 mRNA is abundant in brain and translates into a partial agonist/antagonist for FGF-5 neurotrophic activity.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

An alternatively spliced fibroblast growth factor (FGF)-5 mRNA is abundant in brain and translates into a partial agonist/antagonist for FGF-5 neurotrophic activity. / Ozawa, K; Suzuki, S; Asada, M; Tomooka, Y; Li, A J; Yoneda, A; Komi, A; Imamura, T.

I: Journal of Biological Chemistry, Bind 273, Nr. 44, 1998, s. 29262-71.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Ozawa, K, Suzuki, S, Asada, M, Tomooka, Y, Li, AJ, Yoneda, A, Komi, A & Imamura, T 1998, 'An alternatively spliced fibroblast growth factor (FGF)-5 mRNA is abundant in brain and translates into a partial agonist/antagonist for FGF-5 neurotrophic activity.', Journal of Biological Chemistry, bind 273, nr. 44, s. 29262-71.

APA

Ozawa, K., Suzuki, S., Asada, M., Tomooka, Y., Li, A. J., Yoneda, A., Komi, A., & Imamura, T. (1998). An alternatively spliced fibroblast growth factor (FGF)-5 mRNA is abundant in brain and translates into a partial agonist/antagonist for FGF-5 neurotrophic activity. Journal of Biological Chemistry, 273(44), 29262-71.

Vancouver

Ozawa K, Suzuki S, Asada M, Tomooka Y, Li AJ, Yoneda A o.a. An alternatively spliced fibroblast growth factor (FGF)-5 mRNA is abundant in brain and translates into a partial agonist/antagonist for FGF-5 neurotrophic activity. Journal of Biological Chemistry. 1998;273(44):29262-71.

Author

Ozawa, K ; Suzuki, S ; Asada, M ; Tomooka, Y ; Li, A J ; Yoneda, A ; Komi, A ; Imamura, T. / An alternatively spliced fibroblast growth factor (FGF)-5 mRNA is abundant in brain and translates into a partial agonist/antagonist for FGF-5 neurotrophic activity. I: Journal of Biological Chemistry. 1998 ; Bind 273, Nr. 44. s. 29262-71.

Bibtex

@article{9e169f205ee411dd8d9f000ea68e967b,
title = "An alternatively spliced fibroblast growth factor (FGF)-5 mRNA is abundant in brain and translates into a partial agonist/antagonist for FGF-5 neurotrophic activity.",
abstract = "We detected in the brain and then cloned two novel, short forms of human and mouse fibroblast growth factor (FGF)-5 mRNA, which were designated human FGF-5S (hFGF-5S) and mouse FGF-5S (mFGF-5S), respectively. Genomic analysis indicated that mFGF-5S and authentic mFGF-5 mRNAs were transcribed from a single gene; hFGF-5S and mFGF-5S mRNAs were generated by excluding the second exon of the respective FGF-5 genes, and the alternatively spliced mRNAs encoded for 123- and 121-amino acid proteins, respectively. Indeed, a neuron-like cell line expressing mFGF-5S mRNA secreted a protein of the expected size and with FGF-5 antigenicity. In PC12 cells, expression of hFGF-5 or exposure to hFGF-5 protein induced differentiation. Neither expression of hFGF-5S, alone, nor co-expression of hFGF-5S with hFGF-5 induced significant differentiation. At high concentrations, hFGF-5S protein partially antagonized FGF-5 activity, whereas by itself, hFGF-5S exerted very weak neurotrophic activity. hFGF-5S protein binds to FGF receptor (FGFR)-1 on PC12 transfectants and partially inhibits hFGF-5-induced tyrosine phosphorylation of FGFR-1 and an FGFR substrate, but it also induces phosphorylation by itself. These results suggest that FGF-5S is a naturally expressed partial agonist/antagonist of FGF-5 neurotrophic activity in the brain and that its effects are exerted in part at the level of the receptor.",
author = "K Ozawa and S Suzuki and M Asada and Y Tomooka and Li, {A J} and A Yoneda and A Komi and T Imamura",
note = "Keywords: Alternative Splicing; Amino Acid Sequence; Animals; Base Sequence; Brain; Cell Differentiation; Cloning, Molecular; DNA, Complementary; Escherichia coli; Fibroblast Growth Factor 5; Fibroblast Growth Factors; Humans; Mice; Molecular Sequence Data; PC12 Cells; Protein Binding; RNA, Messenger; Rats; Receptors, Fibroblast Growth Factor; Recombinant Proteins; Ribonucleases",
year = "1998",
language = "English",
volume = "273",
pages = "29262--71",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "44",

}

RIS

TY - JOUR

T1 - An alternatively spliced fibroblast growth factor (FGF)-5 mRNA is abundant in brain and translates into a partial agonist/antagonist for FGF-5 neurotrophic activity.

AU - Ozawa, K

AU - Suzuki, S

AU - Asada, M

AU - Tomooka, Y

AU - Li, A J

AU - Yoneda, A

AU - Komi, A

AU - Imamura, T

N1 - Keywords: Alternative Splicing; Amino Acid Sequence; Animals; Base Sequence; Brain; Cell Differentiation; Cloning, Molecular; DNA, Complementary; Escherichia coli; Fibroblast Growth Factor 5; Fibroblast Growth Factors; Humans; Mice; Molecular Sequence Data; PC12 Cells; Protein Binding; RNA, Messenger; Rats; Receptors, Fibroblast Growth Factor; Recombinant Proteins; Ribonucleases

PY - 1998

Y1 - 1998

N2 - We detected in the brain and then cloned two novel, short forms of human and mouse fibroblast growth factor (FGF)-5 mRNA, which were designated human FGF-5S (hFGF-5S) and mouse FGF-5S (mFGF-5S), respectively. Genomic analysis indicated that mFGF-5S and authentic mFGF-5 mRNAs were transcribed from a single gene; hFGF-5S and mFGF-5S mRNAs were generated by excluding the second exon of the respective FGF-5 genes, and the alternatively spliced mRNAs encoded for 123- and 121-amino acid proteins, respectively. Indeed, a neuron-like cell line expressing mFGF-5S mRNA secreted a protein of the expected size and with FGF-5 antigenicity. In PC12 cells, expression of hFGF-5 or exposure to hFGF-5 protein induced differentiation. Neither expression of hFGF-5S, alone, nor co-expression of hFGF-5S with hFGF-5 induced significant differentiation. At high concentrations, hFGF-5S protein partially antagonized FGF-5 activity, whereas by itself, hFGF-5S exerted very weak neurotrophic activity. hFGF-5S protein binds to FGF receptor (FGFR)-1 on PC12 transfectants and partially inhibits hFGF-5-induced tyrosine phosphorylation of FGFR-1 and an FGFR substrate, but it also induces phosphorylation by itself. These results suggest that FGF-5S is a naturally expressed partial agonist/antagonist of FGF-5 neurotrophic activity in the brain and that its effects are exerted in part at the level of the receptor.

AB - We detected in the brain and then cloned two novel, short forms of human and mouse fibroblast growth factor (FGF)-5 mRNA, which were designated human FGF-5S (hFGF-5S) and mouse FGF-5S (mFGF-5S), respectively. Genomic analysis indicated that mFGF-5S and authentic mFGF-5 mRNAs were transcribed from a single gene; hFGF-5S and mFGF-5S mRNAs were generated by excluding the second exon of the respective FGF-5 genes, and the alternatively spliced mRNAs encoded for 123- and 121-amino acid proteins, respectively. Indeed, a neuron-like cell line expressing mFGF-5S mRNA secreted a protein of the expected size and with FGF-5 antigenicity. In PC12 cells, expression of hFGF-5 or exposure to hFGF-5 protein induced differentiation. Neither expression of hFGF-5S, alone, nor co-expression of hFGF-5S with hFGF-5 induced significant differentiation. At high concentrations, hFGF-5S protein partially antagonized FGF-5 activity, whereas by itself, hFGF-5S exerted very weak neurotrophic activity. hFGF-5S protein binds to FGF receptor (FGFR)-1 on PC12 transfectants and partially inhibits hFGF-5-induced tyrosine phosphorylation of FGFR-1 and an FGFR substrate, but it also induces phosphorylation by itself. These results suggest that FGF-5S is a naturally expressed partial agonist/antagonist of FGF-5 neurotrophic activity in the brain and that its effects are exerted in part at the level of the receptor.

M3 - Journal article

C2 - 9786939

VL - 273

SP - 29262

EP - 29271

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 44

ER -

ID: 5277399