Forskning ved Københavns Universitet - Københavns Universitet

Forside

An Arabidopsis thaliana knock-out mutant of the chloroplast triose phosphate/phosphate translocator is severely compromised only when starch synthesis, but not starch mobilisation is abolished

Publikation: Bidrag til tidsskriftTidsskriftartikelForskning

Standard

An Arabidopsis thaliana knock-out mutant of the chloroplast triose phosphate/phosphate translocator is severely compromised only when starch synthesis, but not starch mobilisation is abolished. / Schneider, Anja; Häusler, Rainer E; Kolukisaoglu, Uner; Kunze, Reinhard; van der Graaff, Eric; Schwacke, Rainer; Catoni, Elisabetta; Desimone, Marcelo; Flügge, Ulf-Ingo.

I: Plant Journal, Bind 32, Nr. 5, 12.2002, s. 685-99.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskning

Harvard

Schneider, A, Häusler, RE, Kolukisaoglu, U, Kunze, R, van der Graaff, E, Schwacke, R, Catoni, E, Desimone, M & Flügge, U-I 2002, 'An Arabidopsis thaliana knock-out mutant of the chloroplast triose phosphate/phosphate translocator is severely compromised only when starch synthesis, but not starch mobilisation is abolished', Plant Journal, bind 32, nr. 5, s. 685-99.

APA

Schneider, A., Häusler, R. E., Kolukisaoglu, U., Kunze, R., van der Graaff, E., Schwacke, R., ... Flügge, U-I. (2002). An Arabidopsis thaliana knock-out mutant of the chloroplast triose phosphate/phosphate translocator is severely compromised only when starch synthesis, but not starch mobilisation is abolished. Plant Journal, 32(5), 685-99.

Vancouver

Schneider A, Häusler RE, Kolukisaoglu U, Kunze R, van der Graaff E, Schwacke R o.a. An Arabidopsis thaliana knock-out mutant of the chloroplast triose phosphate/phosphate translocator is severely compromised only when starch synthesis, but not starch mobilisation is abolished. Plant Journal. 2002 dec;32(5):685-99.

Author

Schneider, Anja ; Häusler, Rainer E ; Kolukisaoglu, Uner ; Kunze, Reinhard ; van der Graaff, Eric ; Schwacke, Rainer ; Catoni, Elisabetta ; Desimone, Marcelo ; Flügge, Ulf-Ingo. / An Arabidopsis thaliana knock-out mutant of the chloroplast triose phosphate/phosphate translocator is severely compromised only when starch synthesis, but not starch mobilisation is abolished. I: Plant Journal. 2002 ; Bind 32, Nr. 5. s. 685-99.

Bibtex

@article{1ed0cf69af664c72add7e8fbce06c8cb,
title = "An Arabidopsis thaliana knock-out mutant of the chloroplast triose phosphate/phosphate translocator is severely compromised only when starch synthesis, but not starch mobilisation is abolished",
abstract = "The Arabidopsis thaliana tpt-1 mutant which is defective in the chloroplast triose phosphate/phosphate translocator (TPT) was isolated by reverse genetics. It contains a T-DNA insertion 24 bp upstream of the start ATG of the TPT gene. The mutant lacks TPT transcripts and triose phosphate (TP)-specific transport activities are reduced to below 5{\%} of the wild type. Analyses of diurnal variations in the contents of starch, soluble sugars and phosphorylated intermediates combined with 14CO2 labelling studies showed, that the lack of TP export for cytosolic sucrose biosynthesis was almost fully compensated by both continuous accelerated starch turnover and export of neutral sugars from the stroma throughout the day. The utilisation of glucose 6-phosphate (generated from exported glucose) rather than TP for sucrose biosynthesis in the light bypasses the key regulatory step catalysed by cytosolic fructose 1,6-bisphosphatase. Despite its regulatory role in the feed-forward control of sucrose biosynthesis, variations in the fructose 2,6-bisphosphate content upon illumination were similar in the mutant and the wild type. Crosses of tpt-1 with mutants unable to mobilise starch (sex1) or to synthesise starch (adg1-1) revealed that growth and photosynthesis of the double mutants was severely impaired only when starch biosynthesis, but not its mobilisation, was affected. For tpt-1/sex1 combining a lack in the TPT with a deficiency in starch mobilisation, an additional compensatory mechanism emerged, i.e. the formation and (most likely) fast turnover of high molecular weight polysaccharides. Steady-state RNA levels and transport activities of other phosphate translocators capable of transporting TP remained unaffected in the mutants.",
keywords = "Arabidopsis, Arabidopsis Proteins, Biological Transport, Carbon Dioxide, Chloroplast Proteins, Chloroplasts, Fructosediphosphates, Gene Deletion, Gene Expression Regulation, Plant, Light, Membrane Transport Proteins, Periodicity, Phenotype, Photosynthesis, Plant Proteins, Starch",
author = "Anja Schneider and H{\"a}usler, {Rainer E} and Uner Kolukisaoglu and Reinhard Kunze and {van der Graaff}, Eric and Rainer Schwacke and Elisabetta Catoni and Marcelo Desimone and Ulf-Ingo Fl{\"u}gge",
year = "2002",
month = "12",
language = "English",
volume = "32",
pages = "685--99",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - An Arabidopsis thaliana knock-out mutant of the chloroplast triose phosphate/phosphate translocator is severely compromised only when starch synthesis, but not starch mobilisation is abolished

AU - Schneider, Anja

AU - Häusler, Rainer E

AU - Kolukisaoglu, Uner

AU - Kunze, Reinhard

AU - van der Graaff, Eric

AU - Schwacke, Rainer

AU - Catoni, Elisabetta

AU - Desimone, Marcelo

AU - Flügge, Ulf-Ingo

PY - 2002/12

Y1 - 2002/12

N2 - The Arabidopsis thaliana tpt-1 mutant which is defective in the chloroplast triose phosphate/phosphate translocator (TPT) was isolated by reverse genetics. It contains a T-DNA insertion 24 bp upstream of the start ATG of the TPT gene. The mutant lacks TPT transcripts and triose phosphate (TP)-specific transport activities are reduced to below 5% of the wild type. Analyses of diurnal variations in the contents of starch, soluble sugars and phosphorylated intermediates combined with 14CO2 labelling studies showed, that the lack of TP export for cytosolic sucrose biosynthesis was almost fully compensated by both continuous accelerated starch turnover and export of neutral sugars from the stroma throughout the day. The utilisation of glucose 6-phosphate (generated from exported glucose) rather than TP for sucrose biosynthesis in the light bypasses the key regulatory step catalysed by cytosolic fructose 1,6-bisphosphatase. Despite its regulatory role in the feed-forward control of sucrose biosynthesis, variations in the fructose 2,6-bisphosphate content upon illumination were similar in the mutant and the wild type. Crosses of tpt-1 with mutants unable to mobilise starch (sex1) or to synthesise starch (adg1-1) revealed that growth and photosynthesis of the double mutants was severely impaired only when starch biosynthesis, but not its mobilisation, was affected. For tpt-1/sex1 combining a lack in the TPT with a deficiency in starch mobilisation, an additional compensatory mechanism emerged, i.e. the formation and (most likely) fast turnover of high molecular weight polysaccharides. Steady-state RNA levels and transport activities of other phosphate translocators capable of transporting TP remained unaffected in the mutants.

AB - The Arabidopsis thaliana tpt-1 mutant which is defective in the chloroplast triose phosphate/phosphate translocator (TPT) was isolated by reverse genetics. It contains a T-DNA insertion 24 bp upstream of the start ATG of the TPT gene. The mutant lacks TPT transcripts and triose phosphate (TP)-specific transport activities are reduced to below 5% of the wild type. Analyses of diurnal variations in the contents of starch, soluble sugars and phosphorylated intermediates combined with 14CO2 labelling studies showed, that the lack of TP export for cytosolic sucrose biosynthesis was almost fully compensated by both continuous accelerated starch turnover and export of neutral sugars from the stroma throughout the day. The utilisation of glucose 6-phosphate (generated from exported glucose) rather than TP for sucrose biosynthesis in the light bypasses the key regulatory step catalysed by cytosolic fructose 1,6-bisphosphatase. Despite its regulatory role in the feed-forward control of sucrose biosynthesis, variations in the fructose 2,6-bisphosphate content upon illumination were similar in the mutant and the wild type. Crosses of tpt-1 with mutants unable to mobilise starch (sex1) or to synthesise starch (adg1-1) revealed that growth and photosynthesis of the double mutants was severely impaired only when starch biosynthesis, but not its mobilisation, was affected. For tpt-1/sex1 combining a lack in the TPT with a deficiency in starch mobilisation, an additional compensatory mechanism emerged, i.e. the formation and (most likely) fast turnover of high molecular weight polysaccharides. Steady-state RNA levels and transport activities of other phosphate translocators capable of transporting TP remained unaffected in the mutants.

KW - Arabidopsis

KW - Arabidopsis Proteins

KW - Biological Transport

KW - Carbon Dioxide

KW - Chloroplast Proteins

KW - Chloroplasts

KW - Fructosediphosphates

KW - Gene Deletion

KW - Gene Expression Regulation, Plant

KW - Light

KW - Membrane Transport Proteins

KW - Periodicity

KW - Phenotype

KW - Photosynthesis

KW - Plant Proteins

KW - Starch

M3 - Journal article

C2 - 12472685

VL - 32

SP - 685

EP - 699

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 5

ER -

ID: 106391574