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Antimicrobial resistance profile of sorbitol non–fermenting Shiga toxin producing Escherichia coli isolated from smallholdings cattle

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Cattle and other ruminants are natural reservoir of sorbitol non–fermenting shiga toxin producing Escherichia coli (SNF–STEC), and human beings can be infected through food chain. Likewise, anti-microbial resistant strain of animal origin may be transmitted to human. The objectives of this study were to determine the antimicrobial resistance profile of SNF–STEC isolated from smallholdings cattle. A total of 57 SNF E. coli isolates were tested, of which 88%, 84% and 82% were sensitive to chloramphenicol, gentamicin and ciprofloxacin, respectively. All the isolates (100%) were resistant to penicillin, whereas, 53% were resistant to trimethoprim–sulfamethoxazole. Among the 57 SNF E. coli, 28 were shiga toxin producing (carrying stx1 or stx2 gene) which exhibited the highest resistance (57%) against trimethoprim–sulfamethoxazole and tetracycline, and the lowest (4%) against chloramphenicol. Of the Stx1 genotypic isolates, 60% and 40% were resistant to trimethoprim–sulfamethoxazole and ampicillin, respectively. On the other hand, 58% Stx2 genotypic isolates were resistant to tetracycline, whereas 44% both of hly and eae genotypic isolates were resistant to trimethoprim–sulfamethoxazole. About 60% isolates carrying all the three STEC virulent genes were resistant to ≥ 2 antimicrobials; among them one isolate was resistant to six antimicrobials – ceftriaxone, nalidixic acid, ciprofloxacin, tetracycline, doxycycline and trimethoprim–sulfamethoxazole. Isolates having no virulent gene, but harboring 54.2 kb sized plasmid were resistant to tetracycline. sulfamethoxazole, ampicillin and amoxicillin. Antimicrobial resistance profile of sorbitol non–fermenting Shiga toxin producing Escherichia coli isolated from smallholdings cattle
OriginalsprogEngelsk
TidsskriftJournal of Infection and Molecular Biology
Vol/bind1
Udgave nummer4
Sider (fra-til)58-63
ISSN2307-5716
StatusUdgivet - 25 sep. 2013
Eksternt udgivetJa

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