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Antisense properties of peptide nucleic acid

Publikation: Bidrag til tidsskriftReviewForskningfagfællebedømt

Peptide nucleic acid (PNA) is a nucleic acid mimic in which the deoxyribose phosphate backbone has been replaced by a pseudo-peptide polymer to which the nucleobases are linked. PNA-oligomers can be synthesized in relatively large amounts, are highly stable in biological environments, and bind complementary DNA and RNA targets with remarkably high affinity and specificity. Thus PNA possesses many of the properties desired for a good antisense agent. Until recently, limited uptake of PNA into cells has been the major obstacle for applying PNA as an antisense agent in cell cultures and in vivo. Here, the antisense properties of PNA in vitro and in vivo will be reviewed. In particular, we will focus on recent observations indicating that PNA equipped with or without various uptake moieties may function as an efficient and gene-specific inhibitor of translation in Escherichia coli and in certain mammalian cell types.

OriginalsprogEngelsk
TidsskriftB B A - Reviews on Cancer
Vol/bind1489
Udgave nummer1
Sider (fra-til)159-66
Antal sider8
ISSN0006-3002
DOI
StatusUdgivet - 10 dec. 1999

ID: 203635701