Forskning ved Københavns Universitet - Københavns Universitet

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Automated extraction of DNA and PCR setup using a Tecan Freedom EVO® liquid handler

Publikation: Bidrag til bog/antologi/rapportKonferencebidrag i proceedingsForskningfagfællebedømt

Michael Stangegaard, Tobias G. Frøslev, Rune Frank-Hansen, Susan Laursen, Mads Jørgensen, Anders Johannes Hansen, Niels Morling

We have implemented and validated automated methods for DNA extraction and PCR setup developed for a Tecan Freedom EVO« liquid handler mounted with a Te-MagS(TM) magnetic separation device. The DNA was extracted using the Qiagen MagAttract« DNA Mini M48 kit. The DNA was amplified using AmpFlSTR« Identifiler«, Y-filer« (Applied Biosystems), GenePrint« FFFL and PowerPlex« Y (Promega). The methods were validated for fresh whole blood and blood from deceased according to EN/ISO 17025
OriginalsprogEngelsk
TitelProgress in Forensic Genetics 13 : Proceedings from the 23rd International ISFG Congress
RedaktørerNiels Morling
Antal sider3
Vol/bindE-Pub
ForlagElsevier
Publikationsdato2009
Sider74-76
DOI
StatusUdgivet - 2009
Begivenhed23rd International ISFG Congress - Buenos Aires, Argentina
Varighed: 14 sep. 200918 sep. 2009

Konference

Konference23rd International ISFG Congress
LandArgentina
ByBuenos Aires
Periode14/09/200918/09/2009
NavnForensic Science International: Genetics Supplement Series
Nummer1
Vol/bind2
ISSN1875-1768

ID: 14439504