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Automated extraction of DNA from blood and PCR setup using a Tecan Freedom EVO liquid handler for forensic genetic STR typing of reference samples

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Automated extraction of DNA from blood and PCR setup using a Tecan Freedom EVO liquid handler for forensic genetic STR typing of reference samples. / Stangegaard, Michael; Frøslev, Tobias G; Frank-Hansen, Rune; Hansen, Anders J; Morling, Niels.

I: Journal of laboratory automation, Bind 16, Nr. 2, 01.04.2011, s. 134-40.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Stangegaard, M, Frøslev, TG, Frank-Hansen, R, Hansen, AJ & Morling, N 2011, 'Automated extraction of DNA from blood and PCR setup using a Tecan Freedom EVO liquid handler for forensic genetic STR typing of reference samples', Journal of laboratory automation, bind 16, nr. 2, s. 134-40. https://doi.org/10.1016/j.jala.2010.11.003

APA

Stangegaard, M., Frøslev, T. G., Frank-Hansen, R., Hansen, A. J., & Morling, N. (2011). Automated extraction of DNA from blood and PCR setup using a Tecan Freedom EVO liquid handler for forensic genetic STR typing of reference samples. Journal of laboratory automation, 16(2), 134-40. https://doi.org/10.1016/j.jala.2010.11.003

Vancouver

Stangegaard M, Frøslev TG, Frank-Hansen R, Hansen AJ, Morling N. Automated extraction of DNA from blood and PCR setup using a Tecan Freedom EVO liquid handler for forensic genetic STR typing of reference samples. Journal of laboratory automation. 2011 apr 1;16(2):134-40. https://doi.org/10.1016/j.jala.2010.11.003

Author

Stangegaard, Michael ; Frøslev, Tobias G ; Frank-Hansen, Rune ; Hansen, Anders J ; Morling, Niels. / Automated extraction of DNA from blood and PCR setup using a Tecan Freedom EVO liquid handler for forensic genetic STR typing of reference samples. I: Journal of laboratory automation. 2011 ; Bind 16, Nr. 2. s. 134-40.

Bibtex

@article{e5e9df7da08a46e5a0cf933d2e5abb49,
title = "Automated extraction of DNA from blood and PCR setup using a Tecan Freedom EVO liquid handler for forensic genetic STR typing of reference samples",
abstract = "We have implemented and validated automated protocols for DNA extraction and PCR setup using a Tecan Freedom EVO liquid handler mounted with the Te-MagS magnetic separation device (Tecan, M{\"a}nnedorf, Switzerland). The protocols were validated for accredited forensic genetic work according to ISO 17025 using the Qiagen MagAttract DNA Mini M48 kit (Qiagen GmbH, Hilden, Germany) from fresh whole blood and blood from deceased individuals. The workflow was simplified by returning the DNA extracts to the original tubes minimizing the risk of misplacing samples. The tubes that originally contained the samples were washed with MilliQ water before the return of the DNA extracts. The PCR was setup in 96-well microtiter plates. The methods were validated for the kits: AmpFlSTR Identifiler, SGM Plus and Yfiler (Applied Biosystems, Foster City, CA), GenePrint FFFL and PowerPlex Y (Promega, Madison, WI). The automated protocols allowed for extraction and addition of PCR master mix of 96 samples within 3.5h. In conclusion, we demonstrated that (1) DNA extraction with magnetic beads and (2) PCR setup for accredited, forensic genetic short tandem repeat typing can be implemented on a simple automated liquid handler leading to the reduction of manual work, and increased quality and throughput.",
author = "Michael Stangegaard and Fr{\o}slev, {Tobias G} and Rune Frank-Hansen and Hansen, {Anders J} and Niels Morling",
note = "Journal of Laboratory Automation 2211-0682 Formerly (until 2011) Association for Laboratory Automation. Journal, The 1535-5535",
year = "2011",
month = "4",
day = "1",
doi = "10.1016/j.jala.2010.11.003",
language = "English",
volume = "16",
pages = "134--40",
journal = "SLAS TECHNOLOGY: Translating Life Sciences Innovation",
issn = "2472-6303",
publisher = "SAGE Publications",
number = "2",

}

RIS

TY - JOUR

T1 - Automated extraction of DNA from blood and PCR setup using a Tecan Freedom EVO liquid handler for forensic genetic STR typing of reference samples

AU - Stangegaard, Michael

AU - Frøslev, Tobias G

AU - Frank-Hansen, Rune

AU - Hansen, Anders J

AU - Morling, Niels

N1 - Journal of Laboratory Automation 2211-0682 Formerly (until 2011) Association for Laboratory Automation. Journal, The 1535-5535

PY - 2011/4/1

Y1 - 2011/4/1

N2 - We have implemented and validated automated protocols for DNA extraction and PCR setup using a Tecan Freedom EVO liquid handler mounted with the Te-MagS magnetic separation device (Tecan, Männedorf, Switzerland). The protocols were validated for accredited forensic genetic work according to ISO 17025 using the Qiagen MagAttract DNA Mini M48 kit (Qiagen GmbH, Hilden, Germany) from fresh whole blood and blood from deceased individuals. The workflow was simplified by returning the DNA extracts to the original tubes minimizing the risk of misplacing samples. The tubes that originally contained the samples were washed with MilliQ water before the return of the DNA extracts. The PCR was setup in 96-well microtiter plates. The methods were validated for the kits: AmpFlSTR Identifiler, SGM Plus and Yfiler (Applied Biosystems, Foster City, CA), GenePrint FFFL and PowerPlex Y (Promega, Madison, WI). The automated protocols allowed for extraction and addition of PCR master mix of 96 samples within 3.5h. In conclusion, we demonstrated that (1) DNA extraction with magnetic beads and (2) PCR setup for accredited, forensic genetic short tandem repeat typing can be implemented on a simple automated liquid handler leading to the reduction of manual work, and increased quality and throughput.

AB - We have implemented and validated automated protocols for DNA extraction and PCR setup using a Tecan Freedom EVO liquid handler mounted with the Te-MagS magnetic separation device (Tecan, Männedorf, Switzerland). The protocols were validated for accredited forensic genetic work according to ISO 17025 using the Qiagen MagAttract DNA Mini M48 kit (Qiagen GmbH, Hilden, Germany) from fresh whole blood and blood from deceased individuals. The workflow was simplified by returning the DNA extracts to the original tubes minimizing the risk of misplacing samples. The tubes that originally contained the samples were washed with MilliQ water before the return of the DNA extracts. The PCR was setup in 96-well microtiter plates. The methods were validated for the kits: AmpFlSTR Identifiler, SGM Plus and Yfiler (Applied Biosystems, Foster City, CA), GenePrint FFFL and PowerPlex Y (Promega, Madison, WI). The automated protocols allowed for extraction and addition of PCR master mix of 96 samples within 3.5h. In conclusion, we demonstrated that (1) DNA extraction with magnetic beads and (2) PCR setup for accredited, forensic genetic short tandem repeat typing can be implemented on a simple automated liquid handler leading to the reduction of manual work, and increased quality and throughput.

U2 - 10.1016/j.jala.2010.11.003

DO - 10.1016/j.jala.2010.11.003

M3 - Journal article

C2 - 21609694

VL - 16

SP - 134

EP - 140

JO - SLAS TECHNOLOGY: Translating Life Sciences Innovation

JF - SLAS TECHNOLOGY: Translating Life Sciences Innovation

SN - 2472-6303

IS - 2

ER -

ID: 33883283