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Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation

Publikation: Bidrag til bog/antologi/rapportKonferencebidrag i proceedingsForskningfagfællebedømt

Michael Stangegaard, Mads Jørgensen, Anders Johannes Hansen, Niels Morling

We have validated and implemented a protocol for DNA extraction from various types of biological materials using a Qiagen BioRobot EZ1 Workstation. The sample materials included whole blood, blood from deceased, buccal cells on Omni swabs and FTA Cards, blood on FTA Cards and cotton swabs, and muscle biopsies. The DNA extraction was validated according to EN/ISO 17025 for the STR kits AmpFlSTR« Identifiler« and AmpFlSTR« Yfiler« (Applied Biosystems). Of 298 samples extracted, 11 (4%) did not yield acceptable results. In conclusion, we have demonstrated that extraction of DNA from various types of biological material can be performed quickly and without the use of hazardous chemicals, and that the DNA may be successfully STR typed according to the requirements of forensic genetic investigations accredited according to EN/ISO 17025
OriginalsprogEngelsk
TitelProgress in Forensic Genetics 13 : Proceedings of the 23rd International ISFG Congress
RedaktørerNiels Morling
Antal sider2
ForlagElsevier
Publikationsdato2009
Sider69-70
DOI
StatusUdgivet - 2009
Begivenhed23rd International ISFG Congress - Buenos Aires, Argentina
Varighed: 14 sep. 200918 sep. 2009

Konference

Konference23rd International ISFG Congress
LandArgentina
ByBuenos Aires
Periode14/09/200918/09/2009
NavnForensic Science International: Genetics Supplement Series
Nummer1
Vol/bind2
ISSN1875-1768

ID: 14440302