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Biochemical Characterization of CPS-1, a Subclass B3 Metallo-β-Lactamase from a Chryseobacterium piscium Soil Isolate

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  • Dereje Dadi Gudeta
  • Simona Pollini
  • Jean-Denis Docquier
  • Valeria Bortolaia
  • Gian Maria Rossolini
  • Guardabassi, Luca
CPS-1 is a subclass B3 metallo-β-lactamase from a Chryseobacterium piscium isolated from soil, showing 68 % amino acid identity to GOB-1 enzyme. CPS-1 was overproduced in Escherichia coli Rosetta (DE3), purified by chromatography and biochemically characterized. This enzyme exhibits a broad spectrum substrate profile including penicillins, cephalosporins and carbapenems, which overall resembles those of L1, GOB-1 and acquired subclass B3 enzymes AIM-1 and SMB-1.
OriginalsprogEngelsk
TidsskriftAntimicrobial Agents and Chemotherapy
Vol/bind60
Udgave nummer3
Sider (fra-til)1869-1873
ISSN0066-4804
DOI
StatusUdgivet - mar. 2016

ID: 153422526