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Biofilm-forming ability profiling of Staphylococcus aureus and Staphylococcus epidermidis mastitis isolates

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Biofilm-forming ability profiling of Staphylococcus aureus and Staphylococcus epidermidis mastitis isolates. / Oliveira, M; Bexiga, R; Nunes, S F; Carneiro, C; Cavaco, L M; Bernardo, F; Vilela, C L.

I: Veterinary Microbiology, Bind 118, Nr. 1-2, 26.11.2006, s. 133-40.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Oliveira, M, Bexiga, R, Nunes, SF, Carneiro, C, Cavaco, LM, Bernardo, F & Vilela, CL 2006, 'Biofilm-forming ability profiling of Staphylococcus aureus and Staphylococcus epidermidis mastitis isolates', Veterinary Microbiology, bind 118, nr. 1-2, s. 133-40. https://doi.org/10.1016/j.vetmic.2006.07.008

APA

Oliveira, M., Bexiga, R., Nunes, S. F., Carneiro, C., Cavaco, L. M., Bernardo, F., & Vilela, C. L. (2006). Biofilm-forming ability profiling of Staphylococcus aureus and Staphylococcus epidermidis mastitis isolates. Veterinary Microbiology, 118(1-2), 133-40. https://doi.org/10.1016/j.vetmic.2006.07.008

Vancouver

Oliveira M, Bexiga R, Nunes SF, Carneiro C, Cavaco LM, Bernardo F o.a. Biofilm-forming ability profiling of Staphylococcus aureus and Staphylococcus epidermidis mastitis isolates. Veterinary Microbiology. 2006 nov 26;118(1-2):133-40. https://doi.org/10.1016/j.vetmic.2006.07.008

Author

Oliveira, M ; Bexiga, R ; Nunes, S F ; Carneiro, C ; Cavaco, L M ; Bernardo, F ; Vilela, C L. / Biofilm-forming ability profiling of Staphylococcus aureus and Staphylococcus epidermidis mastitis isolates. I: Veterinary Microbiology. 2006 ; Bind 118, Nr. 1-2. s. 133-40.

Bibtex

@article{905b7f75813c4d7bb5317276d087c5ab,
title = "Biofilm-forming ability profiling of Staphylococcus aureus and Staphylococcus epidermidis mastitis isolates",
abstract = "Biofilm-forming ability has been increasingly recognized as an important virulence factor in Staphylococci, facilitating their persistence in the host, evading its defences and allowing bacterial survival at high antimicrobial concentrations. Staphylococcus aureus remains a major pathogen of chronic mastitis, but in the last years Staphylococcus epidermidis has emerged as a relevant mastitis pathogen. The present work aimed at the evaluation of the biofilm-forming ability of Staphylococci field isolates from bovine subclinical mastitis and at the development of a fluorescent in situ hybridisation (FISH) protocol that would allow the direct observation of biofilm formation in milk samples. The analysis of phenotypic expression in Congo Red Agar (CRA) and by FISH, showed that 37.5{\%} of the S. aureus isolates produced biofilm, while by optical density measurement only 18.75{\%} isolates revealed this phenotype. The results showed a fair agreement according to the kappa coefficient test (kappa = 0.259). Regarding S. epidermidis mastitis isolates, 37.5{\%} revealed the ability to produce biofilm, but only four isolates were positive by all methods. This agreement was moderate (kappa = 0.467). The application of FISH to artificially contaminated milk samples allowed the direct observation of biofilm production by 37.5{\%} isolates, showing total agreement with the CRA results. This method better mimics the in vivo conditions, especially in terms of the presence of calcium and iron, which in high concentrations, respectively, are known to inhibit or induce biofilm production.",
keywords = "Animals, Biofilms, Cattle, Colony Count, Microbial, Congo Red, Diagnosis, Differential, Female, In Situ Hybridization, Fluorescence, Mastitis, Bovine, Milk, Sensitivity and Specificity, Staphylococcal Infections, Staphylococcus aureus, Staphylococcus epidermidis, Journal Article, Research Support, Non-U.S. Gov't",
author = "M Oliveira and R Bexiga and Nunes, {S F} and C Carneiro and Cavaco, {L M} and F Bernardo and Vilela, {C L}",
year = "2006",
month = "11",
day = "26",
doi = "10.1016/j.vetmic.2006.07.008",
language = "English",
volume = "118",
pages = "133--40",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Biofilm-forming ability profiling of Staphylococcus aureus and Staphylococcus epidermidis mastitis isolates

AU - Oliveira, M

AU - Bexiga, R

AU - Nunes, S F

AU - Carneiro, C

AU - Cavaco, L M

AU - Bernardo, F

AU - Vilela, C L

PY - 2006/11/26

Y1 - 2006/11/26

N2 - Biofilm-forming ability has been increasingly recognized as an important virulence factor in Staphylococci, facilitating their persistence in the host, evading its defences and allowing bacterial survival at high antimicrobial concentrations. Staphylococcus aureus remains a major pathogen of chronic mastitis, but in the last years Staphylococcus epidermidis has emerged as a relevant mastitis pathogen. The present work aimed at the evaluation of the biofilm-forming ability of Staphylococci field isolates from bovine subclinical mastitis and at the development of a fluorescent in situ hybridisation (FISH) protocol that would allow the direct observation of biofilm formation in milk samples. The analysis of phenotypic expression in Congo Red Agar (CRA) and by FISH, showed that 37.5% of the S. aureus isolates produced biofilm, while by optical density measurement only 18.75% isolates revealed this phenotype. The results showed a fair agreement according to the kappa coefficient test (kappa = 0.259). Regarding S. epidermidis mastitis isolates, 37.5% revealed the ability to produce biofilm, but only four isolates were positive by all methods. This agreement was moderate (kappa = 0.467). The application of FISH to artificially contaminated milk samples allowed the direct observation of biofilm production by 37.5% isolates, showing total agreement with the CRA results. This method better mimics the in vivo conditions, especially in terms of the presence of calcium and iron, which in high concentrations, respectively, are known to inhibit or induce biofilm production.

AB - Biofilm-forming ability has been increasingly recognized as an important virulence factor in Staphylococci, facilitating their persistence in the host, evading its defences and allowing bacterial survival at high antimicrobial concentrations. Staphylococcus aureus remains a major pathogen of chronic mastitis, but in the last years Staphylococcus epidermidis has emerged as a relevant mastitis pathogen. The present work aimed at the evaluation of the biofilm-forming ability of Staphylococci field isolates from bovine subclinical mastitis and at the development of a fluorescent in situ hybridisation (FISH) protocol that would allow the direct observation of biofilm formation in milk samples. The analysis of phenotypic expression in Congo Red Agar (CRA) and by FISH, showed that 37.5% of the S. aureus isolates produced biofilm, while by optical density measurement only 18.75% isolates revealed this phenotype. The results showed a fair agreement according to the kappa coefficient test (kappa = 0.259). Regarding S. epidermidis mastitis isolates, 37.5% revealed the ability to produce biofilm, but only four isolates were positive by all methods. This agreement was moderate (kappa = 0.467). The application of FISH to artificially contaminated milk samples allowed the direct observation of biofilm production by 37.5% isolates, showing total agreement with the CRA results. This method better mimics the in vivo conditions, especially in terms of the presence of calcium and iron, which in high concentrations, respectively, are known to inhibit or induce biofilm production.

KW - Animals

KW - Biofilms

KW - Cattle

KW - Colony Count, Microbial

KW - Congo Red

KW - Diagnosis, Differential

KW - Female

KW - In Situ Hybridization, Fluorescence

KW - Mastitis, Bovine

KW - Milk

KW - Sensitivity and Specificity

KW - Staphylococcal Infections

KW - Staphylococcus aureus

KW - Staphylococcus epidermidis

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.vetmic.2006.07.008

DO - 10.1016/j.vetmic.2006.07.008

M3 - Journal article

C2 - 16920280

VL - 118

SP - 133

EP - 140

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 1-2

ER -

ID: 183246795