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Biomek®-3000 and GenPlex SNP Genotyping in Forensic Genetics

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Standard

Biomek®-3000 and GenPlex SNP Genotyping in Forensic Genetics. / Stangegaard, Michael; Tomas, Carmen; Hansen, Anders J.; Frank-Hansen, Rune; Børsting, Claus; Morling, Niels.

I: JALA - Journal of the Association for Laboratory Automation, Bind 13, Nr. 5, 2008, s. 297-303.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Stangegaard, M, Tomas, C, Hansen, AJ, Frank-Hansen, R, Børsting, C & Morling, N 2008, 'Biomek®-3000 and GenPlex SNP Genotyping in Forensic Genetics', JALA - Journal of the Association for Laboratory Automation, bind 13, nr. 5, s. 297-303. https://doi.org/doi:10.1016/j.jala.2008.05.006

APA

Stangegaard, M., Tomas, C., Hansen, A. J., Frank-Hansen, R., Børsting, C., & Morling, N. (2008). Biomek®-3000 and GenPlex SNP Genotyping in Forensic Genetics. JALA - Journal of the Association for Laboratory Automation, 13(5), 297-303. https://doi.org/doi:10.1016/j.jala.2008.05.006

Vancouver

Stangegaard M, Tomas C, Hansen AJ, Frank-Hansen R, Børsting C, Morling N. Biomek®-3000 and GenPlex SNP Genotyping in Forensic Genetics. JALA - Journal of the Association for Laboratory Automation. 2008;13(5):297-303. https://doi.org/doi:10.1016/j.jala.2008.05.006

Author

Stangegaard, Michael ; Tomas, Carmen ; Hansen, Anders J. ; Frank-Hansen, Rune ; Børsting, Claus ; Morling, Niels. / Biomek®-3000 and GenPlex SNP Genotyping in Forensic Genetics. I: JALA - Journal of the Association for Laboratory Automation. 2008 ; Bind 13, Nr. 5. s. 297-303.

Bibtex

@article{d72edf10a1fb11debc73000ea68e967b,
title = "Biomek{\circledR}-3000 and GenPlex SNP Genotyping in Forensic Genetics",
abstract = "Single nucleotide polymorphism genotyping provides a supplement for conventional short tandem repeats-based kits currently used for human identification. GenPlex (Applied Biosystems (AB), Foster City, CA) is an SNP-genotyping kit based on a multiplex of 48 informative, autosomal SNPs from the SNPforID Consortium. Our objective was to setup, implement, and validate a small and affordable automated liquid-handling robot for forensic casework samples (buccal swaps on FTA-paper and Qiagen purified blood). The reaction scheme consisted of numerous steps and was cumbersome to perform consistently manually. Automation was accomplished with a Biomek-3000 (Beckmann Coulter) laboratory-automated workstation using five in-house-developed methods. All methods allowed the user to select the number of subsequent injections to the capillary electrophoresis instrument (ABI 3130xl, AB) enabling processing of both partial and full plates. A total of 286 samples were analyzed in duplicates with the GenPlex reaction using the Biomek-3000. The results were compared with those obtained from the same samples using the SNaPshot(AB) single-base extension system. Full concordance of the results was obtained in all but one sample. The results demonstrate that the Biomek-3000 can perform a series of complex reactions leading to highly consistent forensic genetic SNP-typing results Udgivelsesdato: 2008/10",
author = "Michael Stangegaard and Carmen Tomas and Hansen, {Anders J.} and Rune Frank-Hansen and Claus B{\o}rsting and Niels Morling",
year = "2008",
doi = "doi:10.1016/j.jala.2008.05.006",
language = "English",
volume = "13",
pages = "297--303",
journal = "SLAS TECHNOLOGY: Translating Life Sciences Innovation",
issn = "2472-6303",
publisher = "SAGE Publications",
number = "5",

}

RIS

TY - JOUR

T1 - Biomek®-3000 and GenPlex SNP Genotyping in Forensic Genetics

AU - Stangegaard, Michael

AU - Tomas, Carmen

AU - Hansen, Anders J.

AU - Frank-Hansen, Rune

AU - Børsting, Claus

AU - Morling, Niels

PY - 2008

Y1 - 2008

N2 - Single nucleotide polymorphism genotyping provides a supplement for conventional short tandem repeats-based kits currently used for human identification. GenPlex (Applied Biosystems (AB), Foster City, CA) is an SNP-genotyping kit based on a multiplex of 48 informative, autosomal SNPs from the SNPforID Consortium. Our objective was to setup, implement, and validate a small and affordable automated liquid-handling robot for forensic casework samples (buccal swaps on FTA-paper and Qiagen purified blood). The reaction scheme consisted of numerous steps and was cumbersome to perform consistently manually. Automation was accomplished with a Biomek-3000 (Beckmann Coulter) laboratory-automated workstation using five in-house-developed methods. All methods allowed the user to select the number of subsequent injections to the capillary electrophoresis instrument (ABI 3130xl, AB) enabling processing of both partial and full plates. A total of 286 samples were analyzed in duplicates with the GenPlex reaction using the Biomek-3000. The results were compared with those obtained from the same samples using the SNaPshot(AB) single-base extension system. Full concordance of the results was obtained in all but one sample. The results demonstrate that the Biomek-3000 can perform a series of complex reactions leading to highly consistent forensic genetic SNP-typing results Udgivelsesdato: 2008/10

AB - Single nucleotide polymorphism genotyping provides a supplement for conventional short tandem repeats-based kits currently used for human identification. GenPlex (Applied Biosystems (AB), Foster City, CA) is an SNP-genotyping kit based on a multiplex of 48 informative, autosomal SNPs from the SNPforID Consortium. Our objective was to setup, implement, and validate a small and affordable automated liquid-handling robot for forensic casework samples (buccal swaps on FTA-paper and Qiagen purified blood). The reaction scheme consisted of numerous steps and was cumbersome to perform consistently manually. Automation was accomplished with a Biomek-3000 (Beckmann Coulter) laboratory-automated workstation using five in-house-developed methods. All methods allowed the user to select the number of subsequent injections to the capillary electrophoresis instrument (ABI 3130xl, AB) enabling processing of both partial and full plates. A total of 286 samples were analyzed in duplicates with the GenPlex reaction using the Biomek-3000. The results were compared with those obtained from the same samples using the SNaPshot(AB) single-base extension system. Full concordance of the results was obtained in all but one sample. The results demonstrate that the Biomek-3000 can perform a series of complex reactions leading to highly consistent forensic genetic SNP-typing results Udgivelsesdato: 2008/10

U2 - doi:10.1016/j.jala.2008.05.006

DO - doi:10.1016/j.jala.2008.05.006

M3 - Journal article

VL - 13

SP - 297

EP - 303

JO - SLAS TECHNOLOGY: Translating Life Sciences Innovation

JF - SLAS TECHNOLOGY: Translating Life Sciences Innovation

SN - 2472-6303

IS - 5

ER -

ID: 14440048