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Breaking tolerance in hepatitis B surface antigen (HBsAg) transgenic mice by vaccination with cross-reactive, natural HBsAg variants

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Breaking tolerance in hepatitis B surface antigen (HBsAg) transgenic mice by vaccination with cross-reactive, natural HBsAg variants. / Schirmbeck, Reinhold; Dikopoulos, Nektarios; Kwissa, Marcin; Leithäuser, Frank; Lamberth, Kasper; Buus, Søren; Melber, Karl; Reimann, Jörg.

I: European Journal of Immunology, Bind 33, Nr. 12, 2003, s. 3342-52.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Schirmbeck, R, Dikopoulos, N, Kwissa, M, Leithäuser, F, Lamberth, K, Buus, S, Melber, K & Reimann, J 2003, 'Breaking tolerance in hepatitis B surface antigen (HBsAg) transgenic mice by vaccination with cross-reactive, natural HBsAg variants', European Journal of Immunology, bind 33, nr. 12, s. 3342-52. https://doi.org/10.1002/eji.200324403

APA

Schirmbeck, R., Dikopoulos, N., Kwissa, M., Leithäuser, F., Lamberth, K., Buus, S., ... Reimann, J. (2003). Breaking tolerance in hepatitis B surface antigen (HBsAg) transgenic mice by vaccination with cross-reactive, natural HBsAg variants. European Journal of Immunology, 33(12), 3342-52. https://doi.org/10.1002/eji.200324403

Vancouver

Schirmbeck R, Dikopoulos N, Kwissa M, Leithäuser F, Lamberth K, Buus S o.a. Breaking tolerance in hepatitis B surface antigen (HBsAg) transgenic mice by vaccination with cross-reactive, natural HBsAg variants. European Journal of Immunology. 2003;33(12):3342-52. https://doi.org/10.1002/eji.200324403

Author

Schirmbeck, Reinhold ; Dikopoulos, Nektarios ; Kwissa, Marcin ; Leithäuser, Frank ; Lamberth, Kasper ; Buus, Søren ; Melber, Karl ; Reimann, Jörg. / Breaking tolerance in hepatitis B surface antigen (HBsAg) transgenic mice by vaccination with cross-reactive, natural HBsAg variants. I: European Journal of Immunology. 2003 ; Bind 33, Nr. 12. s. 3342-52.

Bibtex

@article{05566760ebcb11ddbf70000ea68e967b,
title = "Breaking tolerance in hepatitis B surface antigen (HBsAg) transgenic mice by vaccination with cross-reactive, natural HBsAg variants",
abstract = "Processing exogenous hepatitis B surface antigen (HBsAg) of the hepatitis B virus (HBV) generates the K(b)-binding S(208-215) epitope 1; processing endogenous HBsAg generates the K(b)-binding S(190-197) epitope 2. Cross-reactive CD8(+) T cell responses were primed to epitope 1 but not epitope 2 when mice were immunized with natural HBsAg(ayw), or HBsAg(adw2) variants differing within both epitopes by one or two residues. Expression of HBsAg(ayw) from a transgene in the liver renders (HBs-tg) mice tolerant to epitope 1 of HBsAg(ayw). CD8(+) T cells specific for epitope 1 could be primed in HBs-tg mice by HBsAg(adw2); these specific CD8(+) T cells cross-reacted with epitope 1 processed from the transgene-encoded HBsAg(ayw). The liver of vaccinated HBsAg(ayw) transgenic mice showed severe histopathology and contained functional (IFNgamma-producing), cross-reactive CD8(+) T cells, and vaccinated HBs-tg mice showed reduced antigenemia. Hence, vaccination with natural HBsAg variants from different HBV sero/genotypes can prime cross-reactive, specific CD8(+) T cell immunity that breaks tolerance to HBsAg.",
author = "Reinhold Schirmbeck and Nektarios Dikopoulos and Marcin Kwissa and Frank Leith{\"a}user and Kasper Lamberth and S{\o}ren Buus and Karl Melber and J{\"o}rg Reimann",
note = "Keywords: Adoptive Transfer; Amino Acid Sequence; Animals; CD8-Positive T-Lymphocytes; Cell Line; Cross Reactions; Epitopes; Hepatitis B Antibodies; Hepatitis B Surface Antigens; Hepatitis B, Chronic; Immune Tolerance; Liver; Mice; Mice, Inbred C57BL; Mice, Transgenic; Molecular Sequence Data; Spleen; Vaccination",
year = "2003",
doi = "10.1002/eji.200324403",
language = "English",
volume = "33",
pages = "3342--52",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "12",

}

RIS

TY - JOUR

T1 - Breaking tolerance in hepatitis B surface antigen (HBsAg) transgenic mice by vaccination with cross-reactive, natural HBsAg variants

AU - Schirmbeck, Reinhold

AU - Dikopoulos, Nektarios

AU - Kwissa, Marcin

AU - Leithäuser, Frank

AU - Lamberth, Kasper

AU - Buus, Søren

AU - Melber, Karl

AU - Reimann, Jörg

N1 - Keywords: Adoptive Transfer; Amino Acid Sequence; Animals; CD8-Positive T-Lymphocytes; Cell Line; Cross Reactions; Epitopes; Hepatitis B Antibodies; Hepatitis B Surface Antigens; Hepatitis B, Chronic; Immune Tolerance; Liver; Mice; Mice, Inbred C57BL; Mice, Transgenic; Molecular Sequence Data; Spleen; Vaccination

PY - 2003

Y1 - 2003

N2 - Processing exogenous hepatitis B surface antigen (HBsAg) of the hepatitis B virus (HBV) generates the K(b)-binding S(208-215) epitope 1; processing endogenous HBsAg generates the K(b)-binding S(190-197) epitope 2. Cross-reactive CD8(+) T cell responses were primed to epitope 1 but not epitope 2 when mice were immunized with natural HBsAg(ayw), or HBsAg(adw2) variants differing within both epitopes by one or two residues. Expression of HBsAg(ayw) from a transgene in the liver renders (HBs-tg) mice tolerant to epitope 1 of HBsAg(ayw). CD8(+) T cells specific for epitope 1 could be primed in HBs-tg mice by HBsAg(adw2); these specific CD8(+) T cells cross-reacted with epitope 1 processed from the transgene-encoded HBsAg(ayw). The liver of vaccinated HBsAg(ayw) transgenic mice showed severe histopathology and contained functional (IFNgamma-producing), cross-reactive CD8(+) T cells, and vaccinated HBs-tg mice showed reduced antigenemia. Hence, vaccination with natural HBsAg variants from different HBV sero/genotypes can prime cross-reactive, specific CD8(+) T cell immunity that breaks tolerance to HBsAg.

AB - Processing exogenous hepatitis B surface antigen (HBsAg) of the hepatitis B virus (HBV) generates the K(b)-binding S(208-215) epitope 1; processing endogenous HBsAg generates the K(b)-binding S(190-197) epitope 2. Cross-reactive CD8(+) T cell responses were primed to epitope 1 but not epitope 2 when mice were immunized with natural HBsAg(ayw), or HBsAg(adw2) variants differing within both epitopes by one or two residues. Expression of HBsAg(ayw) from a transgene in the liver renders (HBs-tg) mice tolerant to epitope 1 of HBsAg(ayw). CD8(+) T cells specific for epitope 1 could be primed in HBs-tg mice by HBsAg(adw2); these specific CD8(+) T cells cross-reacted with epitope 1 processed from the transgene-encoded HBsAg(ayw). The liver of vaccinated HBsAg(ayw) transgenic mice showed severe histopathology and contained functional (IFNgamma-producing), cross-reactive CD8(+) T cells, and vaccinated HBs-tg mice showed reduced antigenemia. Hence, vaccination with natural HBsAg variants from different HBV sero/genotypes can prime cross-reactive, specific CD8(+) T cell immunity that breaks tolerance to HBsAg.

U2 - 10.1002/eji.200324403

DO - 10.1002/eji.200324403

M3 - Journal article

C2 - 14635042

VL - 33

SP - 3342

EP - 3352

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 12

ER -

ID: 9943580