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Cancer-specific binary expression system activated in mice by bacteriophage HK022 Integrase

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Amer Elias
  • Itay Spector
  • Ilana Sogolovsky-Bard
  • Natalia Gritsenko
  • Lene Rask
  • Yuli Mainbakh
  • Yael Zilberstein
  • Ezra Yagil
  • Mikhail Kolot

Binary systems based on site-specific recombination have been used for tumor specific transcription targeting of suicide genes in animal models. In these binary systems a site specific recombinase or integrase that is expressed from a tumor specific promoter drives tumor specific expression of a cytotoxic gene. In the present study we developed a new cancer specific binary expression system activated by the Integrase (Int) of the lambdoid phage HK022. We demonstrate the validity of this system by the specific expression of a luciferase (luc) reporter in human embryonic kidney 293T (HEK293T) cells and in a lung cancer mouse model. Due to the absence viral vectors and of cytotoxicity the Int based binary system offers advantages over previously described counterparts and may therefore be developed into a safer cancer cell killing system.

OriginalsprogEngelsk
Artikelnummer24971
TidsskriftScientific Reports
Vol/bind6
Antal sider10
ISSN2045-2322
DOI
StatusUdgivet - 27 apr. 2016

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