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Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication

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Standard

Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication. / Baum, Oliver; Torchetti, Eleonora; Malik, Corinna; Høier, Birgitte; Walker, Meegan; Walker, Philip J; Odriozola, Adolfo; Graber, Franziska; Tschanz, Stefan A; Bangsbo, Jens; Hoppeler, Hans H; Askew, Christopher D; Hellsten, Ylva.

I: American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, Bind 310, Nr. 10, 2016, s. R943-R951.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Baum, O, Torchetti, E, Malik, C, Høier, B, Walker, M, Walker, PJ, Odriozola, A, Graber, F, Tschanz, SA, Bangsbo, J, Hoppeler, HH, Askew, CD & Hellsten, Y 2016, 'Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication', American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, bind 310, nr. 10, s. R943-R951. https://doi.org/10.1152/ajpregu.00480.2015

APA

Baum, O., Torchetti, E., Malik, C., Høier, B., Walker, M., Walker, P. J., ... Hellsten, Y. (2016). Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, 310(10), R943-R951. https://doi.org/10.1152/ajpregu.00480.2015

Vancouver

Baum O, Torchetti E, Malik C, Høier B, Walker M, Walker PJ o.a. Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology. 2016;310(10):R943-R951. https://doi.org/10.1152/ajpregu.00480.2015

Author

Baum, Oliver ; Torchetti, Eleonora ; Malik, Corinna ; Høier, Birgitte ; Walker, Meegan ; Walker, Philip J ; Odriozola, Adolfo ; Graber, Franziska ; Tschanz, Stefan A ; Bangsbo, Jens ; Hoppeler, Hans H ; Askew, Christopher D ; Hellsten, Ylva. / Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication. I: American Journal of Physiology: Regulatory, Integrative and Comparative Physiology. 2016 ; Bind 310, Nr. 10. s. R943-R951.

Bibtex

@article{e2009e4ed9894e27addd2e8022ef6226,
title = "Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication",
abstract = "Intermittent claudication (IC) is the most commonly reported symptom of peripheral arterial disease (PAD). Impaired limb blood flow is a major casual factor of lower exercise tolerance in PAD, but cannot entirely explain it. We hypothesized that IC is associated with structural changes of the capillary-mitochondria interface that could contribute to the reduction of exercise tolerance in IC-patients. Capillary and mitochondrial morphometry were performed after light and transmission electron microscopy using vastus lateralis muscle biopsies of 14 IC-patients and 10 age-matched controls and peak power output (PPO) was determined for all participants using an incremental single-leg knee-extension protocol. Capillary density was lower (411±90 mm(-2)versus 506±95 mm(-2); P≤0.05) in the biopsies of the IC patients than in those of the controls. The basement membrane (BM) around capillaries was thicker (543±82 nm versus 423±97 nm; P≤0.01) and the volume density of mitochondria was lower (3.51±0.56{\%} versus 4.60±0.74; P≤0.01) in the IC-patients than the controls. In the IC-patients, a higher proportion of capillaries appeared with collapsed slit-like lumen and/or swollen endothelium. PPO was lower (18.5±9.9 W versus 33.5±9.4 W; P≤0.01) in the IC-patients than the controls. We suggest that several structural alterations in skeletal muscle, either collectively or separately, contribute to the reduction of exercise tolerance in IC-patients.",
author = "Oliver Baum and Eleonora Torchetti and Corinna Malik and Birgitte H{\o}ier and Meegan Walker and Walker, {Philip J} and Adolfo Odriozola and Franziska Graber and Tschanz, {Stefan A} and Jens Bangsbo and Hoppeler, {Hans H} and Askew, {Christopher D} and Ylva Hellsten",
note = "CURIS 2016 NEXS 133",
year = "2016",
doi = "10.1152/ajpregu.00480.2015",
language = "English",
volume = "310",
pages = "R943--R951",
journal = "American Journal of Physiology: Regulatory, Integrative and Comparative Physiology",
issn = "0363-6119",
publisher = "American Physiological Society",
number = "10",

}

RIS

TY - JOUR

T1 - Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication

AU - Baum, Oliver

AU - Torchetti, Eleonora

AU - Malik, Corinna

AU - Høier, Birgitte

AU - Walker, Meegan

AU - Walker, Philip J

AU - Odriozola, Adolfo

AU - Graber, Franziska

AU - Tschanz, Stefan A

AU - Bangsbo, Jens

AU - Hoppeler, Hans H

AU - Askew, Christopher D

AU - Hellsten, Ylva

N1 - CURIS 2016 NEXS 133

PY - 2016

Y1 - 2016

N2 - Intermittent claudication (IC) is the most commonly reported symptom of peripheral arterial disease (PAD). Impaired limb blood flow is a major casual factor of lower exercise tolerance in PAD, but cannot entirely explain it. We hypothesized that IC is associated with structural changes of the capillary-mitochondria interface that could contribute to the reduction of exercise tolerance in IC-patients. Capillary and mitochondrial morphometry were performed after light and transmission electron microscopy using vastus lateralis muscle biopsies of 14 IC-patients and 10 age-matched controls and peak power output (PPO) was determined for all participants using an incremental single-leg knee-extension protocol. Capillary density was lower (411±90 mm(-2)versus 506±95 mm(-2); P≤0.05) in the biopsies of the IC patients than in those of the controls. The basement membrane (BM) around capillaries was thicker (543±82 nm versus 423±97 nm; P≤0.01) and the volume density of mitochondria was lower (3.51±0.56% versus 4.60±0.74; P≤0.01) in the IC-patients than the controls. In the IC-patients, a higher proportion of capillaries appeared with collapsed slit-like lumen and/or swollen endothelium. PPO was lower (18.5±9.9 W versus 33.5±9.4 W; P≤0.01) in the IC-patients than the controls. We suggest that several structural alterations in skeletal muscle, either collectively or separately, contribute to the reduction of exercise tolerance in IC-patients.

AB - Intermittent claudication (IC) is the most commonly reported symptom of peripheral arterial disease (PAD). Impaired limb blood flow is a major casual factor of lower exercise tolerance in PAD, but cannot entirely explain it. We hypothesized that IC is associated with structural changes of the capillary-mitochondria interface that could contribute to the reduction of exercise tolerance in IC-patients. Capillary and mitochondrial morphometry were performed after light and transmission electron microscopy using vastus lateralis muscle biopsies of 14 IC-patients and 10 age-matched controls and peak power output (PPO) was determined for all participants using an incremental single-leg knee-extension protocol. Capillary density was lower (411±90 mm(-2)versus 506±95 mm(-2); P≤0.05) in the biopsies of the IC patients than in those of the controls. The basement membrane (BM) around capillaries was thicker (543±82 nm versus 423±97 nm; P≤0.01) and the volume density of mitochondria was lower (3.51±0.56% versus 4.60±0.74; P≤0.01) in the IC-patients than the controls. In the IC-patients, a higher proportion of capillaries appeared with collapsed slit-like lumen and/or swollen endothelium. PPO was lower (18.5±9.9 W versus 33.5±9.4 W; P≤0.01) in the IC-patients than the controls. We suggest that several structural alterations in skeletal muscle, either collectively or separately, contribute to the reduction of exercise tolerance in IC-patients.

U2 - 10.1152/ajpregu.00480.2015

DO - 10.1152/ajpregu.00480.2015

M3 - Journal article

C2 - 27009051

VL - 310

SP - R943-R951

JO - American Journal of Physiology: Regulatory, Integrative and Comparative Physiology

JF - American Journal of Physiology: Regulatory, Integrative and Comparative Physiology

SN - 0363-6119

IS - 10

ER -

ID: 160055416