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Carriage and fecal counts of cefotaxime M-producing Escherichia coli in pigs: a longitudinal study

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Carriage and fecal counts of cefotaxime M-producing Escherichia coli in pigs : a longitudinal study. / Hansen, Katrine Hartung; Damborg, Peter Panduro; Andreasen, Margit; Nielsen, Søren Saxmose; Guardabassi, Luca.

I: Applied and Environmental Microbiology, Bind 79, Nr. 3, 2013, s. 794-798.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Hansen, KH, Damborg, PP, Andreasen, M, Nielsen, SS & Guardabassi, L 2013, 'Carriage and fecal counts of cefotaxime M-producing Escherichia coli in pigs: a longitudinal study', Applied and Environmental Microbiology, bind 79, nr. 3, s. 794-798. https://doi.org/10.1128/AEM.02399-12

APA

Hansen, K. H., Damborg, P. P., Andreasen, M., Nielsen, S. S., & Guardabassi, L. (2013). Carriage and fecal counts of cefotaxime M-producing Escherichia coli in pigs: a longitudinal study. Applied and Environmental Microbiology, 79(3), 794-798. https://doi.org/10.1128/AEM.02399-12

Vancouver

Hansen KH, Damborg PP, Andreasen M, Nielsen SS, Guardabassi L. Carriage and fecal counts of cefotaxime M-producing Escherichia coli in pigs: a longitudinal study. Applied and Environmental Microbiology. 2013;79(3):794-798. https://doi.org/10.1128/AEM.02399-12

Author

Hansen, Katrine Hartung ; Damborg, Peter Panduro ; Andreasen, Margit ; Nielsen, Søren Saxmose ; Guardabassi, Luca. / Carriage and fecal counts of cefotaxime M-producing Escherichia coli in pigs : a longitudinal study. I: Applied and Environmental Microbiology. 2013 ; Bind 79, Nr. 3. s. 794-798.

Bibtex

@article{6310731131ce48c3826de58ca4397d4d,
title = "Carriage and fecal counts of cefotaxime M-producing Escherichia coli in pigs: a longitudinal study",
abstract = "Current knowledge on extended-spectrum beta-lactamases (ESBLs) in animals is based largely on cross-sectional studies and qualitative data. The aim of this longitudinal study was to elucidate carriage proportions and fecal counts of ESBL-producing Escherichia coli in pigs during the production cycle. At each of three ESBL-positive single-sited farrow-to-finisher pig farms (farms A, B, and C) included in the study, individual fecal samples were taken from 17 to 20 sows 1 week before farrowing and from 2 piglets of each sow's litter four times from birth to slaughter (as piglets, weaners, and finishers). Cefotaxime (CTX)-resistant coliforms in feces were counted on MacConkey agar containing 2 µg/ml CTX and characterized for the presence of ESBL-encoding genes by PCR and sequencing. CTX-M-positive pigs were detected in all age groups at farms A (bla(CTX-M-9) group, compatible with bla(CTX-M-14/17)) and B (bla(CTX-M-1) group, compatible with bla(CTX-M-1/61)), whereas only three weaners were positive at farm C (bla(CTX-M-1) group, compatible with bla(CTX-M-1/61)). A significant decrease in carriage was detected during the production cycle, with on average 50{\%} carriage immediately after birth, 58{\%} just before weaning, 29{\%} during weaning, and 12{\%} during finishing. The observed reduction in numbers of CTX-M-positive pigs was accompanied by a significant reduction in mean fecal counts of CTX-resistant coliforms from ~10(7) CFU/g in piglets to ~10(3) CFU/g in finishers (P <0.001). These findings provide novel information about the epidemiology of ESBLs at the farm level and have important implications for assessments of risks of meat contamination during slaughter.",
author = "Hansen, {Katrine Hartung} and Damborg, {Peter Panduro} and Margit Andreasen and Nielsen, {S{\o}ren Saxmose} and Luca Guardabassi",
year = "2013",
doi = "10.1128/AEM.02399-12",
language = "English",
volume = "79",
pages = "794--798",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "3",

}

RIS

TY - JOUR

T1 - Carriage and fecal counts of cefotaxime M-producing Escherichia coli in pigs

T2 - a longitudinal study

AU - Hansen, Katrine Hartung

AU - Damborg, Peter Panduro

AU - Andreasen, Margit

AU - Nielsen, Søren Saxmose

AU - Guardabassi, Luca

PY - 2013

Y1 - 2013

N2 - Current knowledge on extended-spectrum beta-lactamases (ESBLs) in animals is based largely on cross-sectional studies and qualitative data. The aim of this longitudinal study was to elucidate carriage proportions and fecal counts of ESBL-producing Escherichia coli in pigs during the production cycle. At each of three ESBL-positive single-sited farrow-to-finisher pig farms (farms A, B, and C) included in the study, individual fecal samples were taken from 17 to 20 sows 1 week before farrowing and from 2 piglets of each sow's litter four times from birth to slaughter (as piglets, weaners, and finishers). Cefotaxime (CTX)-resistant coliforms in feces were counted on MacConkey agar containing 2 µg/ml CTX and characterized for the presence of ESBL-encoding genes by PCR and sequencing. CTX-M-positive pigs were detected in all age groups at farms A (bla(CTX-M-9) group, compatible with bla(CTX-M-14/17)) and B (bla(CTX-M-1) group, compatible with bla(CTX-M-1/61)), whereas only three weaners were positive at farm C (bla(CTX-M-1) group, compatible with bla(CTX-M-1/61)). A significant decrease in carriage was detected during the production cycle, with on average 50% carriage immediately after birth, 58% just before weaning, 29% during weaning, and 12% during finishing. The observed reduction in numbers of CTX-M-positive pigs was accompanied by a significant reduction in mean fecal counts of CTX-resistant coliforms from ~10(7) CFU/g in piglets to ~10(3) CFU/g in finishers (P <0.001). These findings provide novel information about the epidemiology of ESBLs at the farm level and have important implications for assessments of risks of meat contamination during slaughter.

AB - Current knowledge on extended-spectrum beta-lactamases (ESBLs) in animals is based largely on cross-sectional studies and qualitative data. The aim of this longitudinal study was to elucidate carriage proportions and fecal counts of ESBL-producing Escherichia coli in pigs during the production cycle. At each of three ESBL-positive single-sited farrow-to-finisher pig farms (farms A, B, and C) included in the study, individual fecal samples were taken from 17 to 20 sows 1 week before farrowing and from 2 piglets of each sow's litter four times from birth to slaughter (as piglets, weaners, and finishers). Cefotaxime (CTX)-resistant coliforms in feces were counted on MacConkey agar containing 2 µg/ml CTX and characterized for the presence of ESBL-encoding genes by PCR and sequencing. CTX-M-positive pigs were detected in all age groups at farms A (bla(CTX-M-9) group, compatible with bla(CTX-M-14/17)) and B (bla(CTX-M-1) group, compatible with bla(CTX-M-1/61)), whereas only three weaners were positive at farm C (bla(CTX-M-1) group, compatible with bla(CTX-M-1/61)). A significant decrease in carriage was detected during the production cycle, with on average 50% carriage immediately after birth, 58% just before weaning, 29% during weaning, and 12% during finishing. The observed reduction in numbers of CTX-M-positive pigs was accompanied by a significant reduction in mean fecal counts of CTX-resistant coliforms from ~10(7) CFU/g in piglets to ~10(3) CFU/g in finishers (P <0.001). These findings provide novel information about the epidemiology of ESBLs at the farm level and have important implications for assessments of risks of meat contamination during slaughter.

U2 - 10.1128/AEM.02399-12

DO - 10.1128/AEM.02399-12

M3 - Journal article

C2 - 23160131

VL - 79

SP - 794

EP - 798

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 3

ER -

ID: 44276987