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Cell cycle-regulated centers of DNA double-strand break repair

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  • Lisby, Michael
  • Adriana Antúnez de Mayolo
  • Uffe H Mortensen
  • Rodney Rothstein

In eukaryotes, homologous recombination is an important pathway for the repair of DNA double-strand breaks. We have studied this process in living cells in the yeast Saccharomyces cerevisiae using Rad52 as a cell biological marker. In response to DNA damage, Rad52 redistributes itself and forms foci specifically during S phase. We have shown previously that Rad52 foci are centers of DNA repair where multiple DNA double-strand breaks colocalize. Here we report a correlation between the timing of Rad52 focus formation and modification of the Rad52 protein. In addition, we show that the two ends of a double-strand break are held tightly together in the majority of cells. Interestingly, in a small but significant fraction of the S phase cells, the two ends of a break separate suggesting that mechanisms exist to reassociate and align these ends for proper DNA repair.

OriginalsprogEngelsk
TidsskriftCell Cycle
Vol/bind2
Udgave nummer5
Sider (fra-til)479-83
Antal sider5
ISSN1538-4101
StatusUdgivet - 10 sep. 2003

ID: 184396723