Forskning ved Københavns Universitet - Københavns Universitet

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Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Rita Sousa-Nunes
  • Amer Ahmed Rana
  • Ross Kettleborough
  • Brickman, Joshua Mark
  • Melanie Clements
  • Alistair Forrest
  • Sean Grimmond
  • Philip Avner
  • James C Smith
  • Sally L Dunwoodie
  • Rosa S P Beddington

This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.

OriginalsprogEngelsk
TidsskriftGenome Research
Vol/bind13
Udgave nummer12
Sider (fra-til)2609-20
Antal sider12
ISSN1088-9051
DOI
StatusUdgivet - dec. 2003

ID: 129061742