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Chemokine Expression in Murine RPE/Choroid in Response to Systemic Viral Infection and Elevated Levels of Circulating Interferon-γ

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Chemokine Expression in Murine RPE/Choroid in Response to Systemic Viral Infection and Elevated Levels of Circulating Interferon-γ. / Faber, Carsten; Juel, Helene Bæk; Jensen, Benjamin Anderschou Holbech; Christensen, Jan Pravsgaard; Prause, Jan Ulrik; Thomsen, Allan Randrup; Nissen, Mogens Holst.

I: Investigative Ophthalmology & Visual Science, Bind 60, Nr. 1, 2019, s. 192-201.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Faber, C, Juel, HB, Jensen, BAH, Christensen, JP, Prause, JU, Thomsen, AR & Nissen, MH 2019, 'Chemokine Expression in Murine RPE/Choroid in Response to Systemic Viral Infection and Elevated Levels of Circulating Interferon-γ', Investigative Ophthalmology & Visual Science, bind 60, nr. 1, s. 192-201. https://doi.org/10.1167/iovs.18-25721

APA

Faber, C., Juel, H. B., Jensen, B. A. H., Christensen, J. P., Prause, J. U., Thomsen, A. R., & Nissen, M. H. (2019). Chemokine Expression in Murine RPE/Choroid in Response to Systemic Viral Infection and Elevated Levels of Circulating Interferon-γ. Investigative Ophthalmology & Visual Science, 60(1), 192-201. https://doi.org/10.1167/iovs.18-25721

Vancouver

Faber C, Juel HB, Jensen BAH, Christensen JP, Prause JU, Thomsen AR o.a. Chemokine Expression in Murine RPE/Choroid in Response to Systemic Viral Infection and Elevated Levels of Circulating Interferon-γ. Investigative Ophthalmology & Visual Science. 2019;60(1):192-201. https://doi.org/10.1167/iovs.18-25721

Author

Faber, Carsten ; Juel, Helene Bæk ; Jensen, Benjamin Anderschou Holbech ; Christensen, Jan Pravsgaard ; Prause, Jan Ulrik ; Thomsen, Allan Randrup ; Nissen, Mogens Holst. / Chemokine Expression in Murine RPE/Choroid in Response to Systemic Viral Infection and Elevated Levels of Circulating Interferon-γ. I: Investigative Ophthalmology & Visual Science. 2019 ; Bind 60, Nr. 1. s. 192-201.

Bibtex

@article{6a35b9d7a59a4171a83741de2631936b,
title = "Chemokine Expression in Murine RPE/Choroid in Response to Systemic Viral Infection and Elevated Levels of Circulating Interferon-γ",
abstract = "Purpose: To examine how circulating immune mediators in vivo may affect gene and protein expression at the RPE/choroid interface.Methods: Young mice were systemically infected with lymphocytic choriomeningitis virus (LCMV) or continuously infused with IFN-γ. RPE/choroid was isolated and analyzed with whole-transcriptome gene expression microarrays. Selected gene expression findings were validated at the protein level.Results: Both the systemic immune activation from virus infection and the sterile systemically increased level of IFN-γ resulted in increased expression of chemokine ligands, chemokine receptors, and early complement components in isolates of RPE/choroid. These findings were largely absent from LCMV-infected mice deficient in either the interferon α/β receptor or IFN-γ.Conclusions: Together, these findings demonstrate that acute systemic immune activation results in a local response at the RPE/choroid interface that may include chemokine-dependent recruitment of inflammatory cells and engagement of the complement system. This may represent a link between the systemic low-grade inflammation and the retinal pathology observed in several multifactorial entities such as aging, AMD, and diabetes.",
author = "Carsten Faber and Juel, {Helene B{\ae}k} and Jensen, {Benjamin Anderschou Holbech} and Christensen, {Jan Pravsgaard} and Prause, {Jan Ulrik} and Thomsen, {Allan Randrup} and Nissen, {Mogens Holst}",
year = "2019",
doi = "10.1167/iovs.18-25721",
language = "English",
volume = "60",
pages = "192--201",
journal = "Investigative Ophthalmology & Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology",
number = "1",

}

RIS

TY - JOUR

T1 - Chemokine Expression in Murine RPE/Choroid in Response to Systemic Viral Infection and Elevated Levels of Circulating Interferon-γ

AU - Faber, Carsten

AU - Juel, Helene Bæk

AU - Jensen, Benjamin Anderschou Holbech

AU - Christensen, Jan Pravsgaard

AU - Prause, Jan Ulrik

AU - Thomsen, Allan Randrup

AU - Nissen, Mogens Holst

PY - 2019

Y1 - 2019

N2 - Purpose: To examine how circulating immune mediators in vivo may affect gene and protein expression at the RPE/choroid interface.Methods: Young mice were systemically infected with lymphocytic choriomeningitis virus (LCMV) or continuously infused with IFN-γ. RPE/choroid was isolated and analyzed with whole-transcriptome gene expression microarrays. Selected gene expression findings were validated at the protein level.Results: Both the systemic immune activation from virus infection and the sterile systemically increased level of IFN-γ resulted in increased expression of chemokine ligands, chemokine receptors, and early complement components in isolates of RPE/choroid. These findings were largely absent from LCMV-infected mice deficient in either the interferon α/β receptor or IFN-γ.Conclusions: Together, these findings demonstrate that acute systemic immune activation results in a local response at the RPE/choroid interface that may include chemokine-dependent recruitment of inflammatory cells and engagement of the complement system. This may represent a link between the systemic low-grade inflammation and the retinal pathology observed in several multifactorial entities such as aging, AMD, and diabetes.

AB - Purpose: To examine how circulating immune mediators in vivo may affect gene and protein expression at the RPE/choroid interface.Methods: Young mice were systemically infected with lymphocytic choriomeningitis virus (LCMV) or continuously infused with IFN-γ. RPE/choroid was isolated and analyzed with whole-transcriptome gene expression microarrays. Selected gene expression findings were validated at the protein level.Results: Both the systemic immune activation from virus infection and the sterile systemically increased level of IFN-γ resulted in increased expression of chemokine ligands, chemokine receptors, and early complement components in isolates of RPE/choroid. These findings were largely absent from LCMV-infected mice deficient in either the interferon α/β receptor or IFN-γ.Conclusions: Together, these findings demonstrate that acute systemic immune activation results in a local response at the RPE/choroid interface that may include chemokine-dependent recruitment of inflammatory cells and engagement of the complement system. This may represent a link between the systemic low-grade inflammation and the retinal pathology observed in several multifactorial entities such as aging, AMD, and diabetes.

U2 - 10.1167/iovs.18-25721

DO - 10.1167/iovs.18-25721

M3 - Journal article

C2 - 30654385

VL - 60

SP - 192

EP - 201

JO - Investigative Ophthalmology & Visual Science

JF - Investigative Ophthalmology & Visual Science

SN - 0146-0404

IS - 1

ER -

ID: 212166751