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Click chemistry mediated functionalization of vertical nanowires for biological applications

Publikation: Bidrag til tidsskriftLetterForskningfagfællebedømt

Semiconductor nanowires (NWs) are gaining significant importance in various biological applications, such as biosensing and drug delivery. Efficient and controlled immobilization of biomolecules on the NW surface is crucial for many of these applications. Here, we present for the first time the use of the CuI-catalyzed alkyne-azide cycloaddition and its strain-promoted variant for the covalent functionalization of vertical NWs with peptides and proteins. The potential of the approach was demonstrated in two complementary applications of measuring enzyme activity and protein binding, which is of general interest for biological studies. The attachment of a peptide substrate provided NW arrays for the detection of protease activity. In addition, green fluorescent protein was immobilized in a site-specific manner and recognized by antibody binding to demonstrate the proof-of-concept for the use of covalently modified NWs for diagnostic purposes using minute amounts of material.

OriginalsprogEngelsk
TidsskriftChemistry: A European Journal
Vol/bind22
Udgave nummer2
Sider (fra-til)496-500
Antal sider5
ISSN1521-3765
DOI
StatusUdgivet - 2016

ID: 155919243