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Cloning and characterization of a novel L-arabinose isomerase from Bacillus licheniformis

Publikation: Bidrag til tidsskriftTidsskriftartikel

Based on analysis of the genome sequence of Bacillus licheniformis ATCC 14580, an isomerase-encoding gene (araA) was proposed as an L-arabinose isomerase (L-AI). The identified araA gene was cloned from B. licheniformis and overexpressed in Escherichia coli. DNA sequence analysis revealed an open reading frame of 1,422 bp, capable of encoding a polypeptide of 474 amino acid residues with a calculated isoelectric point of pH 4.8 and a molecular mass of 53,500 Da. The gene was overexpressed in E. coli, and the protein was purified as an active soluble form using Ni-NTA chromatography. The molecular mass of the purified enzyme was estimated to be approximately 53 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 113 kDa by gel filtration chromatography, suggesting that the enzyme is a homodimer. The enzyme required a divalent metal ion, either Mn(2+)or Co(2+), for enzymatic activity. The enzyme had an optimal pH and temperature of 7.5 and 50 degrees C, respectively, with a k (cat) of 12,455 min(-1) and a k (cat)/K (m) of 34 min(-1) mM(-1) for L-arabinose, respectively. Although L-AIs have been characterized from several other sources, B. licheniformis L-AI is distinguished from other L-AIs by its wide pH range, high substrate specificity, and catalytic efficiency for L-arabinose, making B. licheniformis L-AI the ideal choice for industrial applications, including enzymatic synthesis of L-ribulose. This work describes one of the most catalytically efficient L-AIs characterized thus far.

OriginalsprogEngelsk
TidsskriftApplied Microbiology and Biotechnology
Vol/bind81
Udgave nummer2
Sider (fra-til)283-290
Antal sider8
ISSN0175-7598
DOI
StatusUdgivet - 2008
Eksternt udgivetJa

ID: 162608628