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Cloning, expression, purification and crystallization of the N-domain from the alpha(2) subunit of the membrane-spanning Na,K-ATPase protein

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The nucleotide-binding domain of the Na,K-ATPase ion pump was expressed with a His tag in Escherichia coli and purified. The soluble 24 kDa derivative consists of 214 amino-acid residues and was crystallized in the presence of NiCl(2). The crystals belong to space group F23, with unit-cell parameters a = b = c = 147.5 A, and diffract to 3.1 A. Complete data sets could be collected from native and thimerosal-treated crystals frozen in 50% sucrose. Five mercury positions were found and initial SIR phases calculated.

OriginalsprogEngelsk
TidsskriftActa crystallographica Section D: Structural biology
Vol/bind59
Udgave nummerPt 7
Sider (fra-til)1259-61
Antal sider3
ISSN0907-4449
StatusUdgivet - jul. 2003

ID: 203907079