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Combination of LC-ICP-MS, LC-MS and NMR for investigation of the oxidative degradation of selenomethionine

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Selenomethionine (SeMet) was oxidized by heating an acidic solution with hydrogen peroxide. Samples were taken before and during the oxidation process. The oxidation products were separated by cation exchange chromatography followed by ICP-MS detection to identify the selenium containing compounds as well as electrospray ionization MS detection to determine the masses of the degradation products. Furthermore, the samples were analyzed by Se-77-NMR. The first appearing degradation product was selenomethionine selenoxide, which was converted via the deaminated selenoxide to methane seleninic acid and selenite. (C) 2003 Elsevier Science B.V. All rights reserved.
OriginalsprogUdefineret/Ukendt
TidsskriftTalanta
Vol/bind59
Udgave nummer6
Sider (fra-til)1165-1171
Antal sider7
ISSN0039-9140
StatusUdgivet - 2003

    Forskningsområder

  • selenomethionine selenoxide selenium speciation mass-spectrometric detection selenium chromatography acid

ID: 38061314