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Comparative Genomic Analyses of Flavobacterium psychrophilum Isolates Reveals New Putative Genetic Determinants of Virulence Traits

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The fish pathogen Flavobacterium psychrophilum is currently one of the main pathogenic bacteria hampering the productivity of salmonid farming worldwide. Although putative virulence determinants have been identified, the genetic basis for variation in virulence of F. psychrophilum is not fully understood. In this study, we analyzed whole-genome sequences of a collection of 25 F. psychrophilum isolates from Baltic Sea countries and compared genomic information with a previous determination of their virulence in juvenile rainbow trout. The results revealed a conserved population of F. psychrophilum that were consistently present across the Baltic Sea countries, with no clear association between genomic repertoire, phylogenomic, or gene distribution and virulence traits. However, analysis of the entire genome of four F. psychrophilum isolates by hybrid assembly pro-vided an unprecedented resolution for discriminating even highly related isolates. The results showed that isolates with different virulence phenotypes harbored genetic variances on a number of consecutive leucine-rich repeat (LRR) proteins, repetitive motifs in gliding motility-associated protein, and the insertion of transposable elements into intergenic and genic regions. Thus, these findings provide novel insights into the genetic variation of these elements and their putative role in the modulation of F. psychrophilum virulence.

OriginalsprogEngelsk
Artikelnummer1658
TidsskriftMicroorganisms
Vol/bind9
Udgave nummer8
Antal sider15
ISSN2076-2607
DOI
StatusUdgivet - 2021

Bibliografisk note

Funding Information:
Funding: BONUS FLAVOPHAGE project supported by BONUS (Art 185), funded jointly by the EU and Innovation Fund Denmark, the Academy of Finland, and the National Centre for Research and Development (NCBiR), Poland. This study was also supported by the Danish Council for Independent Research (Project # DFF-7014–00080).

Funding Information:
BONUS FLAVOPHAGE project supported by BONUS (Art 185), funded jointly by the EU and Innovation Fund Denmark, the Academy of Finland, and the National Centre for Research and Development (NCBiR), Poland. This study was also supported by the Danish Council for Independ-ent Research (Project # DFF-7014?00080).Acknowledgments: We would like to thank Eva Jansson (Sweden), Bettina Schletz and Stephanie Bornstein (Germany), Agnieszka P?kala-Safi?ska (Poland), and Vladimir Voronin (Russia) for providing their isolates, and the fish farmers for allowing us to sample for bacterial isolation.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

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