Forskning ved Københavns Universitet - Københavns Universitet

Forside

Complexin 3 Increases the Fidelity of Signaling in a Retinal Circuit by Regulating Exocytosis at Ribbon Synapses

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Lena S Mortensen
  • Silvia J H Park
  • Jiang-Bin Ke
  • Benjamin H Cooper
  • Lei Zhang
  • Imig, Cordelia
  • Siegrid Löwel
  • Kerstin Reim
  • Nils Brose
  • Jonathan B Demb
  • Jeong-Seop Rhee
  • Joshua H Singer

Complexin (Cplx) proteins modulate the core SNARE complex to regulate exocytosis. To understand the contributions of Cplx to signaling in a well-characterized neural circuit, we investigated how Cplx3, a retina-specific paralog, shapes transmission at rod bipolar (RB)→AII amacrine cell synapses in the mouse retina. Knockout of Cplx3 strongly attenuated fast, phasic Ca(2+)-dependent transmission, dependent on local [Ca(2+)] nanodomains, but enhanced slower Ca(2+)-dependent transmission, dependent on global intraterminal [Ca(2+)] ([Ca(2+)]I). Surprisingly, coordinated multivesicular release persisted at Cplx3(-/-) synapses, although its onset was slowed. Light-dependent signaling at Cplx3(-/-) RB→AII synapses was sluggish, owing largely to increased asynchronous release at light offset. Consequently, propagation of RB output to retinal ganglion cells was suppressed dramatically. Our study links Cplx3 expression with synapse and circuit function in a specific retinal pathway and reveals a role for asynchronous release in circuit gain control.

OriginalsprogEngelsk
TidsskriftCell Reports
Vol/bind15
Udgave nummer10
Sider (fra-til)2239-2250
Antal sider12
ISSN2211-1247
DOI
StatusUdgivet - 7 jun. 2016
Eksternt udgivetJa

ID: 237697975